A5006828330- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Pulmonary Hypertension Research and Treatments
- MicroRNA in disease regulation
- Prostate Cancer Treatment and Research
- RNA modifications and cancer
- RNA Research and Splicing
- Peptidase Inhibition and Analysis
- Single-cell and spatial transcriptomics
- Cancer Genomics and Diagnostics
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Ferroptosis and cancer prognosis
- Blood Coagulation and Thrombosis Mechanisms
- Protease and Inhibitor Mechanisms
- Myasthenia Gravis and Thymoma
- Protein purification and stability
- Pancreatic and Hepatic Oncology Research
- Cancer Cells and Metastasis
- Metabolomics and Mass Spectrometry Studies
- Cancer-related Molecular Pathways
- Molecular Biology Techniques and Applications
- Cardiac Structural Anomalies and Repair
- Genomics and Phylogenetic Studies
- Gut microbiota and health
- Cancer, Lipids, and Metabolism
Chinese Academy of Medical Sciences & Peking Union Medical College
2008-2025
Beijing Hospital
2011-2024
Wenzhou Medical University
2023-2024
First Affiliated Hospital of Wenzhou Medical University
2024
Beijing Anzhen Hospital
2019
Capital Medical University
2019
Beijing Geriatric Hospital
2014-2017
National Clinical Research Center for Digestive Diseases
2015
Circular RNAs (circRNAs) are an abundant class of endogenous non‑coding and associated with numerous diseases, including cancer, cardiovascular type 2 diabetes mellitus (T2DM). However, the association between circRNAs inflammation or inflammatory cytokines in patients T2DM remains to be fully elucidated. The purpose present study was investigate expression profiles peripheral leucocytes their cytokines. Peripheral blood from (n=43) healthy individuals (n=45) were collected for RNA...
Abstract Invasive fungal infections acquired in the hospital have progressively emerged as an important cause of life-threatening infection. In particular, airborne fungi hospitals are considered critical pathogens hospital-associated infections. To identify causative microorganisms, high-volume air samplers were utilized for collection, and species identification was performed using a culture-based method DNA sequencing analysis with Illumina MiSeq HiSeq 2000 systems. Few bacteria grown...
Small extrachromosomal circular DNAs (eccDNAs) have the potential to be cancer biomarkers. However, formation mechanisms and functions of small eccDNAs selected in carcinogenesis are not clear, whether eccDNA profile plasma patients represents that tissues remains elucidated.A novel sequencing workflow based on nanopore platform was used sequence naturally existing full-length collected from 25 (including prostate cancer, hepatocellular carcinoma colorectal cancer), an independent validation...
Classic strategies for circular RNA (circRNA) preparation always introduce large numbers of linear transcripts or extra nucleotides to the circularized product. In this study, we aimed develop an efficient system circRNA based on a self-splicing ribozyme derived from optimized Tetrahymena thermophila group Ⅰ intron. The target sequence was inserted downstream and complementary antisense region added upstream assist cyclization. Then, compared circularization efficiency flanking intronic...
Recombinant human B-type natriuretic peptide (rhBNP) has been extensively proven to be an effective mean of heart failure (HF) therapy, but its clinical application is limited by very short half-life. This study aims combine in vitro transcribed mRNA (IVT mRNA) and fusion protein technology develop a rhBNP-Fc drug with long half-life, high efficiency few side effects treat HF. The IgG4-Fc sequence was produced IVT technology. transfected into HEK293T cells examine the expression vitro....
ABSTRACT The distribution and the function of T lymphocyte subsets in pleural effusion (PE) peripheral blood (PB) tuberculous (TPE) patients remain unclear. In this study, we aimed to explore expression patterns, regulatory mechanisms, functions TPE patients, especially at single cell level. CD3 + cells were isolated from PE PB four for single‐cell RNA sequencing (scRNA‐seq) screen subsets. T‐SNE projection, Gene Set Variation Analysis (GSVA), pseudotime analysis performed analyze...
Lung microbiome ecosystem homeostasis in idiopathic pulmonary fibrosis (IPF) remains uncharacterized. The aims of this study were to identify unique microbial signatures the lung and analyze gene function IPF patients. DNA isolated from BALF samples was obtained for high-throughput sequencing. Microbial metagenomic data used principal component analysis (PCA) analyzed at different taxonomic levels. Shotgun annotated using KEGG database functional metabolic pathways. In study, 17 patients 38...
Vascular endothelial growth factor (VEGF), an important angiogenic that is able to stimulate the proliferation and migration of cells, best-studied hallmark angiogenesis. Neovascularization a major cause age-related macular degeneration (AMD) which leading blindness in elderly population. Specific molecular inhibitors VEGF have been proved be useful treatment AMD. Ranibizumab Bevacizumab are structurally similar anti-VEGF drugs Many studies indicated roughly equal short-term efficacy safety,...
Significance Inhibitory Smads (I-Smads) play important roles to negatively regulate TGF-β/BMP (bone morphogenetic protein) signaling, thus controlling numerous cellular and developmental processes. Recent studies have suggested that Smad7, a member of I-Smads, is overexpressed in cancer types its abundance positively correlated the malignancy. However, molecular mechanism underlying action I-Smads cells remains poorly understood. Here we show Drosophila I-Smad, Dad, accesses membrane via...
BACKGROUND The aim of this study was to evaluate the role urinary sarcosine/creatinine ratio in diagnosis and prognosis prostate cancer, using a sarcosine oxidase assay. MATERIAL AND METHODS Urine samples were obtained from 203 patients with benign hyperplasia (BPH) 209 cancer. Levels measured method. ratios compared between group BPH In two groups, measurement serum prostate-specific antigen (PSA) free/total (F/T) PSA ratio. Correlations Gleason grade stage cancer analyzed. RESULTS There...
BACKGROUND Chronic heart failure (CHF) is a leading cause of death worldwide. A long noncoding RNA (lncRNA) named urothelial carcinoma associated 1 (UCA1) important in multiple diseases. However, the role UCA1 CHF still unknown. Our study investigated whether could be applied as an ideal marker to diagnose and evaluate prognosis CHF. MATERIAL AND METHODS Total plasma was extracted from 67 patients controls. Quantitative real-time polymerase chain reaction used determine level UCA1....
Abstract Background Due to the diversity of immune repertoire (IR), reconstructing full-length IR using traditional short-read sequencing has proven challenging. Methods A (FLIRseq) work flow was developed with linear rolling circle amplification and nanopore sequencing. Its accuracy quantification ability were verified by plasmid mixtures commercial B-cell receptor/T-cell receptor (BCR/TCR-seq) based on short reads. IRs in tissues peripheral blood from 8 patients acute lymphoblastic...
Abstract Background Abiraterone and MDV3100 are two effective anticancer agents for prostate cancer, however, the mechanism of their downstream action remains undefined. Methods A dual fluorescent biosensor plasmid was transfected in LNCaP cells to measure mitophagy. The DNA extracted performed with quantitative real-time PCR detect mitochondrial copy number. JC-1 staining utilized membrane potential electron microscope analyze morphology. Moreover, protein levels markers apoptotic were...
Abstract Doxorubicin is a common chemotherapeutic agent in clinic, but myocardial toxicity limits its use. Fibroblast growth factor (FGF) 10, multifunctional paracrine factor, plays diverse roles embryonic and postnatal heart development as well cardiac regeneration repair. In this study we investigated the role of FGF10 potential modulator doxorubicin-induced cytotoxicity underlying molecular mechanisms. Fgf10 +/− mice an inducible dominant negative FGFR2b transgenic mouse model ( Rosa26...
Abstract Background Thymomas and thymic carcinomas are the most common tumor types among anterior mediastinal lesions. However, relationship between molecular aberrations thymoma patients poorly understood, especially abnormal changes in expression profiles of circRNAs. The purpose present study was to investigate circRNAs their possible roles pathogenesis thymoma. Methods Diseased tissues surrounding normal two were collected for circRNA sequencing. top four upregulated selected as...
The surge of genome sequencing data has underlined substantial genetic variants uncertain significance (VUS). decryption VUS discovered by poses a major challenge in the post-sequencing era. Although experimental assays have progressed classifying VUS, only tiny fraction human genes been explored experimentally. Thus, it is urgently needed to generate state-of-the-art functional predictors silico. Artificial intelligence (AI) an invaluable tool assist identification with high efficiency and...