A5102733693- Advanced Proteomics Techniques and Applications
- Pancreatic and Hepatic Oncology Research
- Caveolin-1 and cellular processes
- Extracellular vesicles in disease
- RNA modifications and cancer
- MicroRNA in disease regulation
- Cell Adhesion Molecules Research
- Ferroptosis and cancer prognosis
- Peptidase Inhibition and Analysis
- Cancer Cells and Metastasis
- Cancer-related molecular mechanisms research
- Cancer, Lipids, and Metabolism
- Cancer Research and Treatments
- RNA Research and Splicing
- Cancer Mechanisms and Therapy
- Immune Cell Function and Interaction
- Impact of Technology on Adolescents
- Nanoplatforms for cancer theranostics
- 3D Printing in Biomedical Research
- Galectins and Cancer Biology
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cancer Genomics and Diagnostics
- Glycosylation and Glycoproteins Research
- Neuroscience and Neural Engineering
China Medical University
2016-2025
First Affiliated Hospital of Guangzhou Medical University
2025
State Key Laboratory of Respiratory Disease
2025
Guangzhou Medical University
2025
Liaoning Cancer Hospital & Institute
2018-2025
Sheng Jing Hospital
2018-2021
Pancreatic cancer is one of the deadliest cancers with rapid disease progression. Further elucidation its underlying molecular mechanisms and novel biomarkers for early detection necessary. Exosomes are small extracellular vesicles that released by multiple cell types acting as message carriers during intercellular communication promising biomarker candidates. However, role pancreatic cell-derived exosomes in progression application these diagnostic have not been fully studied. In this...
Pancreatic cancer is one of the most malignant tumors. Invasion and metastasis can occur in early stage pancreatic cancer, contributing to poor prognosis. Accordingly, this study, we evaluated molecular mechanisms underlying invasion metastasis. Using mass spectrometry, found that Integrin alpha 6 (ITGA6) was more highly expressed a invasive cell line (PC-1.0) than less (PC-1). Through vitro vivo experiments, observed significant decreases cells after inhibiting ITGA6. Based on data TCGA,...
Recent studies have reported direct antitumor effects of mannose, a natural six-carbon monosaccharide, in the treatment cancer. Herein, we utilized cancer cell lines, animal models, organoids and experimental techniques such as multi-omics cellular experiments to investigate regulatory mannose on NSCLC growth inflammatory microenvironment. We demonstrated that can inhibit growth, infiltration cytokine expression tissue, enhance efficacy immune checkpoint inhibitor both vitro vivo. Orally...
Pancreatic cancer is a highly metastatic and chemo-resistant disease. Secreted proteins involved in cell-cell interactions play an important role changing the tumor microenvironment. Previous studies generally focus on secretome of cell line from serum-free media, due to serious interference fetal bovine serum (FBS). However, serum-starvation may alter expression patterns secreted proteins. Hence, efforts decrease proteomic analysis serum-containing media have been hampered quantitatively...
Pancreatic cancer is the fourth leading cause of death, with a 5-year survival rate only 1–4%. Integrin-mediated cell adhesion critical for initiation, progression, and metastasis cancer. In this study we investigated role integrin b4 (ITGB4) its phosphorylation at tyrosine Y1510 (p-ITGB4-Y1510) in tumorigenesis pancreatic We analyzed expression ITGB4 p-ITGB4-Y1510 tissue lines using immunohistochemistry, Western blot, or semi-quantitative reverse transcription PCR. were highly expressed (n...
Pancreatic cancer is characterized by its unsatisfying early detection rate, rapid disease progression and poor prognosis. Further studies on molecular mechanism novel predictive biomarkers for pancreatic based a large sample volume are required. Multiple bioinformatic analysis tools were utilized identification characterization of differentially expressed genes (DEGs) from merged microarray data (100 samples 62 normal samples). Data the GEO TCGA database was to validate diagnostic...
Pancreatic cancer is a malignancy with very poor prognosis. The emergence of liquid biopsy expected to achieve accurate early diagnosis through detection tumor-derived secreted proteins in the blood. Early and treatment pancreatic could help improve prognosis.The pretreatment approach samples can have major effect on downstream analysis. In this study, we used pair homologous cell supernatants different capacities for invasion metastasis examine conditioned media without removal fetal bovine...
Abstract Backgroud Pancreatic cancer is a highly malignant tumor of the digestive system. This secretome pancreatic key to its progression and metastasis. But different methods protein extraction affect final results. In other words, real secretion proteins in cells has been changed. Based on mass spectrometry, we analyze from serum-containing serum-free medium, using pretreatment methods. study aims identify dissociation factors cancer. Methods this study, were cultured or corresponding...
Background: Disrupted gene levels are intimately correlated with the occurrence and prognosis of gastric cancer (GC).As genes do not function in isolation, we set out to investigate possible relationship among mRNA non-coding RNAs (ncRNAs).Materials methods: RNA sequencing from 406 cases GC was acquired through TCGA database.R packages were utilized assess differential expression.The competing endogenous (ceRNA) network predicted using miRcode, miRDB, mirTarBase, Target Scan constructed by...
AIMTo detect significant clusters of co-expressed genes associated with tumorigenesis that might help to predict stomach adenocarcinoma (SA) prognosis.
Pancreatic cancer is characterized by its rapid progression and early metastasis. This requires further elucidation of the key promoters for In this study, we identified REST as hub gene a module which closely associated with stage weighted correlation network analysis. Validation TCGA database, western blot analysis human pancreatic cell lines (AsPC-1, Capan-2, SW-1990, PANC-1) immunohistochemical paraffin-embedded tissue sections showed that was enriched in samples advanced metastatic...
Abstract Pancreatic cancer is a highly malignant tumor of the digestive system. This secretome pancreatic key to its progression and metastasis. In this study, different protein pretreatment methods were used analyze secretome, furthermore identify dissociation factors in cancer. cells cultured serum-containing or serum-free medium, corresponding supernatants extracted as samples. Subsequently, above samples separated by size exclusion chromatography (SEC), peptide segments identified...
Abstract Pancreatic cancer is a highly malignant tumor of the digestive system. This secretome pancreatic key to its progression and metastasis. In this study, different protein pretreatment methods were used analyze secretome, furthermore identify dissociation factors in cancer. cells cultured serum-containing or serum-free medium, corresponding supernatants extracted as samples. Subsequently, above samples separated by size exclusion chromatography (SEC), peptide segments identified...