Pancreatic cancer is one of the deadliest cancers with rapid disease progression. Further elucidation its underlying molecular mechanisms and novel biomarkers for early detection necessary. Exosomes are small extracellular vesicles that released by multiple cell types acting as message carriers during intercellular communication promising biomarker candidates. However, role pancreatic cell-derived exosomes in progression application these diagnostic have not been fully studied. In this...

// Peng Liu 1 , Zhipeng Wang Sarah Brown Vinodh Kannappan Patricia Erebi Tawari Wenguo Jiang 2 Juan M. Irache 3 James Z. Tang Stephen Britland Angel L. Armesilla John Darling Xing 4 and Weiguang Research Institute in Healthcare Science, Faculty of Science & Engineering, University Wolverhampton, UK Cardiff University-Peking Cancer Institute, School Medicine, Henry Wellcome Building, Heath Park, Cardiff, Pharmacy, Navarra, Pamplona, Spain Shenyang Pharmaceutical University, Shenyang, China...

Breast cancer is a major public health problem all over the world, and current treatment strategies are not potent enough for some patients, especially those with triple-negative breast (TNBC). Recent studies have demonstrated that microRNAs (miRNA) play vital roles in development of TNBC. In this study, we found miR-185 was strongly downregulated TNBC tissues cell lines its expression levels were associated lymph node metastasis, clinical stage, overall survival, relapse-free survival We...

Background: Next generation sequencing (NGS)-based multi-gene panel tests have been performed to predict the treatment response and prognosis in patients with colorectal cancer (CRC). Whether mutation results of formalin-fixed paraffin-embedded (FFPE) tissues are identical those fresh frozen remains unknown. Methods: A 22-gene 103 hotspots was used detect mutations paired tissue FFPE from 118 CRC. Results: In our study, 117 (99.2%) had one or more variants, 226 variants 221 tissue. Of 129...

Human amniotic fluid stem cell-derived exosome (HuAFSC-exosome) transplantation is considered a promising treatment for premature ovarian failure (POF). However, its mechanism remains unclear. In this study, exosomes were isolated and enriched from HuAFSC subsets of CD44+/CD105+, the transplanted into POF model in vitro vivo. Our results confirmed that produced by CD44+/CD105+ HuAFSCs could achieve therapeutic effects mouse model. research also showed HuAFSC-exosomes carrying miR-369-3p...

During osteoporosis bone formation by osteoblasts is reduced and/or resorption osteoclasts enhanced. Currently, only a few factors have been identified in the regulation of integrity osteoblast-derived osteocytes. In this study, we show that specific disruption menin, encoded multiple endocrine neoplasia type 1 (Men1), and osteocytes caused despite preservation osteoblast differentiation rate. Instead, an increase osteoclast numbers was detected persisted even when deletion Men1 restricted...

Pancreatic cancer is one of the most malignant tumors. Invasion and metastasis can occur in early stage pancreatic cancer, contributing to poor prognosis. Accordingly, this study, we evaluated molecular mechanisms underlying invasion metastasis. Using mass spectrometry, found that Integrin alpha 6 (ITGA6) was more highly expressed a invasive cell line (PC-1.0) than less (PC-1). Through vitro vivo experiments, observed significant decreases cells after inhibiting ITGA6. Based on data TCGA,...

Background: Perineural invasion (PNI) and lymphovascular (LVI) are associated with poor prognosis in colorectal cancer, but their clinical significance is still controversial for patients locally advanced rectal cancer (LARC) who had received neoadjuvant chemoradiotherapy (nCRT) surgical resection. The aim of this study was to confirm the correlation between PNI and/or LVI further whether can be used as potential prognostic indicators adjuvant chemotherapy after nCRT surgery LARC. Methods:...

The restoration of nerve dysfunction after traumatic brain injury (TBI) faces huge challenges due to the limited self-regenerative abilities tissues. In situ inductive recovery can be achieved utilizing biological scaffolds combined with endogenous human umbilical cord mesenchymal stem cells (HUCMSCs)-derived exosomes (MExos). this study, brain-derived neurotrophic factor-stimulated HUCMSCs-derived (BMExos) were composited collagen/chitosan by 3D printing technology. 3D-printed...

This study aimed to explore the effects of exosomes derived from human gingival mesenchymal stem cells (GMSC-Exo) on inflammatory response periodontal ligament (PDLSCs) in an microenvironment order restore regenerative potential PDLSCs, which promotes tissue regeneration patients with periodontitis. Periodontitis is a chronic infectious disease characterized by inflammation and alveolar bone destruction. PDLSCs are regarded as promising seed for restoring defects because their ability...

Degenerative orthopaedic diseases pose a notable worldwide public health issue attributable to the global aging population. Conventional medical approaches, encompassing physical therapy, pharmaceutical interventions, and surgical methods, face obstacles in halting or reversing degenerative process. In recent times, exosome-based therapy has gained widespread acceptance popularity as an effective treatment for diseases. This therapeutic approach holds potential "cell-free" tissue...

Although research has established individual difference antecedents of voice behavior, little is known about the role differences in psychological needs that affect voice. We draw on theory to propose a needs-driven approach studying behavior. Specifically, we hypothesize individual’s need for affiliation differential effects promotive and prohibitive voice, leader–member exchange (LMX) transmits personal two types group cohesion weakens indirect behavior through LMX. test these hypotheses...

The metastasis of colorectal cancer frequently tends to liver, which is one the three leading causes cancer-related deaths worldwide. Growing evidence showed that aspirin could effectively inhibit liver cancer. However, potential mechanism has not been fully understood. Mouse splenic vein assay was used examine metastatic ability colon cells in vivo. And wound healing and transwell were applied detect C26 HCT116 cell lines vitro. RT-PCR western blotting explore Toll-like receptor 4 (TLR4)...

Berberine has been reported to inhibit cancer cell growth by apoptosis induction and exhibits a protective role against progression. The current study aims investigate the effects of berberine on acute lymphoblastic leukemia (ALL) mechanism beyond apoptosis.Cell viability was determined in ALL lines EU-6 SKW-3 using trypan blue staining. Cell autophagy immunofluorescence Western blot. xenograft mice were established anti-tumor BBR. molecular explored siRNA signaling inhibitors.Herein, we...

Abstract Aim To study the role of exosomes in protective effect cerebral ischemic preconditioning (cerebral‐IPC) against I/R injury. Method Mouse models cerebral‐IPC and MCAO/R were established as described previously, their behavioral, pathological, proteomic changes analyzed. Neuro‐2a subjected to OGD/R treated with isolated from plasma sham‐operated mice. The differentially expressed miRNAs between derived (S‐exosomes) preconditioned (IPC‐exosomes) mice identified through miRNA array,...

Pancreatic cancer is known to have a high mortality rate, and its early diagnosis remains challenging due the occult location of pancreas. Exosomes derived from pancreatic cells specifically express glypican-1, which may provide liquid biopsy opportunity for cancer. Herein, an inductively coupled plasma mass spectrometry (ICP-MS) photothermal dual-readout platform was proposed ultrasensitive point-of-care analysis exosomes. In our design, exosomes were captured by sandwich immunoassay,...

Abstract Atherosclerosis is a complex pathological process involving macrophages, endothelial cells and vascular smooth muscle that can lead to ischemic heart disease; however, the mechanisms underlying cell‐to‐cell communication in atherosclerosis are poorly understood. In this study, we focused on role of exosomal miRNAs crosstalk between macrophages explored rarely studied molecular involved. Our vitro result showed macrophage‐derived miR‐4532 significantly disrupted human umbilical vein...

Primary Immune Thrombocytopenia (ITP) is characterized by reduced immunosuppressive function of regulatory T cells (Tregs), contributing to immune imbalance and decreased platelet counts. However, the mechanisms behind this efficacy Tregs remain unclear. Our study used a variety methods, including Treg assays, cytokine analysis, single-cell sequencing, explore these mechanisms. We found that exosomes from ITP patients inhibited TBX21 expression in Tregs, impaired their ability suppress Th1...

Hepatocellular carcinoma (HCC) is one of the malignancies with increasing incidence globally, and microvascular invasion (MVI) a crucial determinant prognosis in patients. This study aimed to investigate platelet distribution width (PDW) diabetes mellitus as indicators for predicting preoperative MVI HCC, providing more accurate predictive tools clinicians guide treatment strategies improve patient survival quality life. A retrospective was conducted, including 1357 patients who underwent...

Protein O-linked β-N-acetylglucosamine glycosylation (O-GlcNAcylation) regulates many biological processes. Studies have shown that O-GlcNAc modification levels can increase during acute stress and suggested this may contribute to the survival of cell. This study investigated possible effects O-GlcNAcylation regulate glucose metabolism, apoptosis, autophagy in liver after cold stress. Male C57BL/6 mice were exposed conditions (4 °C) for 0, 2, 4, 6 h, then their livers extracted expression...