A5013766857- Cardiac electrophysiology and arrhythmias
- Ion channel regulation and function
- Receptor Mechanisms and Signaling
- Pancreatic function and diabetes
- Cardiac Arrest and Resuscitation
- Diabetes Treatment and Management
- Cardiac Ischemia and Reperfusion
- Heart Rate Variability and Autonomic Control
- Adenosine and Purinergic Signaling
- ECG Monitoring and Analysis
- Cardiovascular Function and Risk Factors
- Nitric Oxide and Endothelin Effects
- Cardiac Arrhythmias and Treatments
- Gut microbiota and health
- Oral microbiology and periodontitis research
- Electrolyte and hormonal disorders
- Acute Myocardial Infarction Research
- Cardiac pacing and defibrillation studies
- Heart rate and cardiovascular health
- Cardiovascular Disease and Adiposity
- Antibiotics Pharmacokinetics and Efficacy
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Diet, Metabolism, and Disease
- Metabolism, Diabetes, and Cancer
- Neutropenia and Cancer Infections
University of Copenhagen
2016-2025
Novo Nordisk Foundation
2017
Capital Region of Denmark
2017
Novo Nordisk Foundation Center for Basic Metabolic Research
2017
University of Southern Denmark
2017
Danish Diabetes Academy
2017
Rigshospitalet
2017
Statens Serum Institut
2017
Steno Diabetes Centers
2017
Abstract Aims Glucagon-like peptide-1 receptor agonists (GLP-1 RAs) are increasingly used to treat type 2 diabetes and obesity. Albeit cardiovascular outcomes generally improve, treatment with GLP-1 RAs is associated increased heart rate, the mechanism of which unclear. Methods results We employed a large animal model, female landrace pig, multiple in vivo ex approaches including pharmacological challenges, electrophysiology, high-resolution mass spectrometry explore how elicits an increase...
Abstract Background Saliva is a protein-rich body fluid for noninvasive discovery of biomolecules, containing both human and microbial components, associated with various chronic diseases. Type-2 diabetes (T2D) imposes significant health socio-economic burden. Prior research on T2D salivary microbiome utilized methods such as metagenomics, metatranscriptomics, 16S rRNA sequencing, low-throughput proteomics. Results We conducted ultrafast, in-depth MS-based proteomic metaproteomic profiling...
Loss-of-function mutations in hERG (encoding the Kv11.1 voltage-gated potassium channel) cause long-QT syndrome type 2 (LQT2) because of prolonged cardiac repolarization. However, is also present pancreatic α and β cells intestinal L K cells, secreting glucagon, insulin, incretins glucagon-like peptide-1 (GLP-1) GIP (glucose-dependent insulinotropic polypeptide), respectively. These hormones are crucial for glucose regulation, may disturbed regulation. We measured secretion these...
Ventricular fibrillation (VF) during acute myocardial infarction (AMI) is an important contributor to sudden cardiac death. Large animal models are widely used study AMI-induced arrhythmia, but the mode of AMI induction ranges from thoracotomy and surgical ligation a coronary vessel (open chest) minimally invasive techniques, including balloon occlusion (closed chest). How choice affects arrhythmia development unclear. The aim this was compare open-chest closed-chest model with regard...
Acute myocardial infarction (AMI) is associated with intracellular Ca2+ build-up. In healthy ventricles, small conductance Ca2+-activated K+ (SK) channels are present but do not participate in repolarization. However, SK current increased chronic and heart failure, recently, channel inhibition was demonstrated to reduce arrhythmias AMI rats. Hence, we hypothesized that inhibitors (NS8593 AP14145) could arrhythmia development during a porcine model.Twenty-seven pigs were randomized 1:1:1...
Potassium Channel Interacting Protein 2 (KChIP2) is suggested to be responsible for the circadian rhythm in repolarization duration, ventricular arrhythmias, and sudden cardiac death. We investigated hypothesis that there no QT interval absence of KChIP2. Implanted telemetric devices recorded electrocardiogram continuously 5 days conscious wild-type mice (WT, n = 9) KChIP2−/− (n light:dark periods complete darkness. intervals were determined from all RR corrected heart rate (QT100...
Abstract The mechanisms behind renal vasodilatation elicited by stimulation of β‐adrenergic receptors are not clarified. As several classes K channels potentially activated, we tested the hypothesis that KV7 and BKCa contribute to decreased vascular tone in vivo vitro. Changes blood flow (RBF) during were measured anaesthetized rats using an ultrasonic probe. isometric tension segmental arteries from normo‐ hypertensive wild‐type mice lacking functional V 7.1 was examined a wire‐myograph....
Ventricular fibrillation (VF) occurring in the first minutes to hours of acute myocardial infarction (AMI) is a frequent cause death and treatment options are limited. The aim was test whether early infusion amiodarone 10 min after onset AMI reduced incidence VF porcine model. Eighteen female Danish landrace pigs were randomized control an group. induced by ligation mid-left anterior descending artery for 120 followed 60 reperfusion. occurred 0/8 treated with compared 7/10 controls (P <...
Background Adenosine leads to atrial action potential (AP) shortening through activation of adenosine 1 receptors (A1-R) and subsequent opening G protein-coupled inwardly rectifying K+ channels. Extracellular production is drastically increased during stress ischemia. Objective The aim this study was address whether the pharmacological blockade endogenous its signaling prevents fibrillation (AF) Methods role A1-R on duration, refractoriness AF vulnerability investigated in rat isolated...
Abstract Loss-of-function (LoF) mutations in KCNQ1 , encoding the voltage-gated K + channel v 7.1, lead to long QT syndrome 1 (LQT1). LQT1 patients also present with post-prandial hyperinsulinemia and hypoglycaemia. In contrast, polymorphisms are associated diabetes, LQTS have a higher prevalence of diabetes. We developed mouse model LoF Kcnq1 mutation using CRISPR-Cas9 hypothesized that this would display prolongation, increased glucose-stimulated insulin secretion allow for interrogation...
Cardiovascular complications, including cardiac arrhythmias, result in high morbidity and mortality patients with type-2 diabetes mellitus (T2DM). Clinical experimental data suggest electrophysiological impairment of the natural pacemaker diabetic heart. The present study examined sinoatrial node (SAN) arrhythmias a mouse model T2DM physiologically probed their underlying cause.Electrocardiograms were obtained from conscious db/db lean control db/+ mice. In vivo SAN function was through...
The voltage-gated potassium channel Kv 11.1 is important for repolarizing the membrane potential in excitable cells such as myocytes, pancreatic α- and β-cells. Moxifloxacin blocks increases risk of hypoglycaemia patients with diabetes. We investigated glucose regulation secretion glucoregulatory hormones young people without moxifloxacin, a drug known to block channel.The effect moxifloxacin (800 mg/day 4 days) or placebo on was assessed randomized, double-blind, crossover study men women...
Adrenaline (epinephrine) is one of the prime messengers fight-or-flight response, favoring activation β-adrenergic receptors. Although general vasoconstriction to nonessential tissues imperative, vasodilatory effect receptor contends with this. We aimed determine dose-dependent effects adrenaline on hemodynamics and test whether could lower blood pressure (BP) through a β2-adrenergic pathway. Nineteen Danish landrace pigs were used pharmacologically probe hemodynamic adrenaline. Pigs...
Patients receiving psychiatric medication, like the antipsychotic drug haloperidol, are at an increased risk of sudden cardiac death (SCD). Haloperidol blocks rapidly-activating delayed rectifier potassium current, thereby increasing electrical dispersion repolarization which can potentially lead to arrhythmias. Whether these patients also a higher develop SCD during acute myocardial infarction (AMI) is unknown. AMI locally shortens action potential duration, might further increase and...
Aim: The underlying mechanisms behind β-adrenergic renal vasodilation is not clarified. Several classes of K + channels are potentially activated, so we tested the hypothesis that voltage-sensitive V 7 and Ca 2+ -activated BK contribute to decrease in vascular tone vivo vitro. Methods: Changes blood flow (RBF) during stimulation was measured isoflurane-anesthetized rats using an ultrasonic probe. isometric tension segmental arteries from normo- hypertensive wild-type mice lacking functional...
Increased left ventricular mass (LVM) is one of the most powerful predictors adverse cardiovascular events. Clinical evaluation requires reliable, accurate and reproducible echocardiographic LVM-quantification to manage patients. For this purpose, we have developed a novel two-dimensional (2D) method based on adding mean wall thickness volume acquired by biplane disks, which has recently been validated in humans using cardiac magnetic resonance as reference value. We assessed hypothesis that...
P<0.05) as well severity of arrhythmic episodes (AS 36.5 6 27.0 vs 8.3 12.3 in RVA 60, P<0.05).Conclusion: Prevention acute bradycardia decreases the probability arrhythmia development induced events.P1022