A5080362489- Hematopoietic Stem Cell Transplantation
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Renal Transplantation Outcomes and Treatments
- Hemoglobinopathies and Related Disorders
- Acute Myeloid Leukemia Research
- Acute Lymphoblastic Leukemia research
- Immunotherapy and Immune Responses
- Transplantation: Methods and Outcomes
- Prenatal Screening and Diagnostics
- Diabetes and associated disorders
- Cytomegalovirus and herpesvirus research
- Immunodeficiency and Autoimmune Disorders
- Blood groups and transfusion
- Polyomavirus and related diseases
- Monoclonal and Polyclonal Antibodies Research
- Organ Transplantation Techniques and Outcomes
- Organ Donation and Transplantation
- vaccines and immunoinformatics approaches
- Aquaculture Nutrition and Growth
- Mesenchymal stem cell research
- Lymphoma Diagnosis and Treatment
- Childhood Cancer Survivors' Quality of Life
- Physiological and biochemical adaptations
- Immune Response and Inflammation
Children's Hospital of Los Angeles
2015-2024
University of Southern California
2016-2024
University of California, San Francisco
2005-2014
University of California San Francisco Medical Center
2013
Columbia University
2011
University of Vermont
2009
Immungenetics (Germany)
2006
Harvard University
2005
Berkeley Public Health Division
2005
University of South Carolina
1996-1998
We describe a four-year experience with bone marrow transplantation involving closely HLA-matched unrelated donors and 55 consecutive patients hematologic disease who were seven months to 48.6 years old (median, 18 years). An intensive pretransplantation conditioning regimen graft-versus-host (GVHD) prophylaxis CD3-directed T-cell depletion cyclosporine employed.
An association has previously been shown between antibiotic-refractory Lyme arthritis, the human histocompatibility leukocyte antigen (HLA)-DR4 molecule, and T cell recognition of an epitope Borrelia burgdorferi outer-surface protein A (OspA163-175). We studied frequencies HLA-DRB1-DQA1-DQB1 haplotypes in 121 patients with or antibiotic-responsive arthritis correlated these vitro binding OspA163-175 peptide to 14 DRB molecules. Among patients, were similar those control subjects. However,...
Killer immunoglobulin-like receptor (KIR)-ligand mismatched natural killer (NK) cells play a key role in achieving durable remission after haplo-identical transplantation for acute myeloid leukaemia. We investigated the feasibility of transfusing haplo-identical, T-cell depleted, KIR-ligand NK cells, conditioning therapy with melphalan and fludarabine, to patients advanced multiple myeloma (MM) followed by delayed rescue autologous stem cells. No graft-versus-host disease or failure engraft...
Abstract The role of haploidentical hematopoietic cell transplantation (HCT) using posttransplant cyclophosphamide (PTCy) for acute lymphoblastic leukemia (ALL) is being defined. We performed a retrospective, multivariable analysis comparing outcomes HCT approaches by donor adults with ALL in remission. primary objective was to compare overall survival (OS) among HCTs PTCy and HLA-matched sibling (MSD), 8/8 unrelated (MUD), 7 /8 HLA-MUD, or umbilical cord blood (UCB) HCT. Comparing MSD HCT,...
A collaborative study involving a large sample of European Americans was typed for the histocompatibility loci HLA DR-DQ region and subjected to intensive typing validation measures in order accurately determine haplotype composition frequency. The resulting tables have immediate application allogeneic transplantation. within are especially valuable such an undertaking because their tight linkage high disequilibrium. 3798 haplotypes, derived from 1899 unrelated individuals, had total 75...
Transplantation-related mortality (TRM) is high after HLA-mismatched umbilical cord blood (UCB) transplantation (UCBT). In utero, exposure to noninherited maternal antigen (NIMA) recognized by the fetus, which induces T regulator cells that haplotype. It plausible UCBTs in recipients are matched donor NIMAs may alleviate some of excess associated with this treatment. To explore concept, we used marginal matched-pair Cox regression analysis compare outcomes 48 NIMA-matched (ie, NIMA UCB unit...
Allogeneic hematopoietic stem cell transplantation (allo-HSCT) with myeloablative conditioning is associated a 10%-40% risk of day +100 transplantation-related mortality (TRM). We evaluated the feasibility and safety reduced-toxicity allo-HSCT in 100 consecutive children adolescent recipients (mean age, 9.2 ± 6.8 years). The mean duration follow-up was 1278 1042 days. Fifty patients had malignant disease. median time to neutrophil recovery 18 days, platelet 43 Median donor chimerism...
Multi-center kidney paired donation (KPD) is an exciting new transplant option that has not yet approached its full potential. One barrier to progress accurate virtual crossmatching for KPD waitlists with many highly sensitized patients. Virtual crossmatch results from a large multi-center consortium, the National Kidney Registry (NKR), were analyzed determine effectiveness of flexible center-specific criteria crossmatching. Approximately two-thirds patients on NKR waitlist are (>80% CPRA)....
Abstract Objective To assess the binding of outer surface protein A (OspA) and human lymphocyte function–associated antigen 1 (hLFA‐1) peptides to 5 major histocompatibility complex (MHC) molecules. Methods Peptide MHC molecules was determined by in vitro assays, correlated with frequencies patients treatment‐resistant Lyme arthritis. Results The HLA–DRB1*0401 molecule bound both OspA 163–175 hLFA‐1α L330–342 well. Although magnitude less, DRB1*0404 also showed peptides. DRB1*0101 well, but...
Alternative donor bone marrow transplantation (BMT) to treat severe aplastic anaemia (SAA) in children and young adults has been complicated by high rates of graft rejection graft‐versus‐host disease (GVHD). We hypothesized that increased immunosuppression combined with T‐cell depletion the would enable successful use unrelated BMT this disease. Preconditioning consisted cytosine arabinoside, cyclophosphamide, total body irradiation (TBI). was anti‐CD3 antibody T10B9. GVHD prophylaxis...
While kidney paired donation (KPD) enables the utilization of living donor kidneys from healthy and willing donors incompatible with their intended recipients, strategy poses complex challenges that have limited its adoption in United States Canada. A consensus conference was convened March 29-30, 2012 to address dynamic complexities KPD inhibit optimal implementation. Stakeholders considered evaluation care, histocompatibility testing, allocation algorithms, financing, geographic...
Summary Immune reconstitution appears to be delayed following myeloablative conditioning (MAC) and umbilical cord blood transplantation (UCBT) in paediatric recipients. Although reduced toxicity (RTC) versus MAC prior allogeneic stem cell is associated with decreased transplant‐related mortality, the effects of RTC UCBT on immune risk graft‐versus‐host disease (GVHD) are unknown. In 88 consecutive recipients UCBT, we assessed recovery immunoglobulin at days +100, 180 365 analysed factors...
For children receiving allogeneic hematopoietic stem cell transplants (HSCTs), the toxicity of conditioning regimen and graft failure remain challenges. We previously reported that targeted i.v. busulfan, fludarabine, rabbit anti-thymocyte globulin (rATG) decreased but had a rate 21%. To improve engraftment rate, we replaced ATG with alemtuzumab, monoclonal Ab targeting CD52. Thirty-five malignant nonmalignant diseases were enrolled in this phase II prospective study. Twelve HLA-matched...
Na,K‐ATPase; Molecular cloning; DNA, complementary; Amino acid sequence; (Artemia)