A5036369564- Heme Oxygenase-1 and Carbon Monoxide
- Cardiac Arrest and Resuscitation
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Sepsis Diagnosis and Treatment
- Cardiac, Anesthesia and Surgical Outcomes
- Thermal Regulation in Medicine
- Liver Disease and Transplantation
- Traumatic Brain Injury and Neurovascular Disturbances
- Renal function and acid-base balance
- Acute Kidney Injury Research
- Organ Transplantation Techniques and Outcomes
- Hemodynamic Monitoring and Therapy
- Acute Ischemic Stroke Management
- Nitric Oxide and Endothelin Effects
- Drug-Induced Hepatotoxicity and Protection
- Phosphodiesterase function and regulation
- Hemoglobin structure and function
- Neonatal Health and Biochemistry
- Neuroscience of respiration and sleep
- Cardiac Ischemia and Reperfusion
- Eicosanoids and Hypertension Pharmacology
- Bacterial Identification and Susceptibility Testing
- Venous Thromboembolism Diagnosis and Management
- Blood Pressure and Hypertension Studies
- Olfactory and Sensory Function Studies
Robert Bosch Hospital
2018-2024
University of Würzburg
2002-2015
Universitätsklinikum Würzburg
2009-2015
Klinik und Poliklinik für Urologie
2011
Lawson Health Research Institute
2002-2004
Western University
2002-2004
Shock is a life-threatening condition characterized by substantial alterations in the microcirculation. This study tests hypothesis that considering sublingual microcirculatory perfusion variables therapeutic management reduces 30-day mortality patients admitted to intensive care unit (ICU) with shock. randomized, prospective clinical multicenter trial-recruited an arterial lactate value above two mmol/L, requiring vasopressors despite adequate fluid resuscitation, regardless of cause All...
In sepsis and systemic inflammation, increased microvascular permeability consecutive breakdown of microcirculatory flow significantly contribute to organ failure death. Evidence points a critical role cAMP levels in endothelial cells maintain capillary barrier properties acute inflammation. However, approaches verify this observation models are rare. Therefore we tested here whether application the phosphodiesterase-4-inhibitors (PD-4-Is) rolipram or roflumilast increase was effective...
To establish the molecular background for glucocorticoid insensitivity, that is, failure to reduce edema formation and protect blood-brain barrier integrity after acute traumatic brain injury.Controlled animal study.University research laboratory.Male C57Bl/6N mice.Mechanical lesion by controlled cortical impact.Our study demonstrates 1) proteasomal receptor degradation is established in endothelial cells injury as a form of posttranslational modification; 2) inhibition pathway with...
The concomitant occurrence of the symptoms intravascular hypovolemia, peripheral edema and hemodynamic instability is typically named Capillary Leak Syndrome (CLS) often occurs in surgical critical ill patients. However, neither a unitary definition nor standardized diagnostic criteria exist so far. We aimed to investigate common characteristics this phenomenon with subsequent scoring system, determining whether CLS contributes mortality.We conducted single-center, observational,...
Breakdown of microvascular endothelial barrier functions contributes to disturbed microcirculation, organ failure, and death in sepsis. Increased cAMP levels by systemic application phosphodiesterase 4 inhibitors (PD-4-I) have previously been demonstrated protect properties a model inflammation (systemic inflammatory response syndrome) suggesting novel therapeutic option overcome this problem. However, clinically relevant polymicrobial sepsis long-term effects, immunomodulatory effects...
The role of haem oxygenase (HO) in the hepatic accumulation leukocytes mice during initiation remote organ injury following normotensive limb ischaemia-reperfusion (I-R) was investigated. Remote initiated by 1 h bilateral hindlimb ischaemia followed either or 1.5 reperfusion male C57BL/6 mice. Mice were randomly assigned to sham (no I-R, n = 4), I-R (n 4 for both time points), plus chromium mesoporphyrin (CrMP, 4) inhibit HO haemin increase HO. Leukocyte and leukocyte-endothelial interaction...
Reactive oxygen species (ROS) are important in the hepatocellular injury process during a systemic inflammation. We examined role of carbon monoxide (CO) on hepatic generation ROS with in-vivo and in-vitro models inflammation.Using murine model bilateral hindlimb ischemia-reperfusion (I/R) we effect CO treatment formation, oxidative status, cell injury. Cultured HUVEC were used to investigate intracellular pathways.CO reduced lipid peroxidation, re-established total glutathione disulfide...
Abstract Background Septic acute liver and intestinal failure is associated with a high mortality. We therefore investigated the influence of volume resuscitation different crystalloid or colloid solutions on intestine injury microcirculation in septic rodents. Methods Sepsis was induced by cecal ligation puncture (CLP) 77 male rats. Animals were treated crystalloids (NaCl 0.9% (NaCl), Ringer’s acetate (RA)) colloids (Gelafundin 4% (Gel), 6% HES 130/0.4 (HES)). After 24 h animals...
Surfactant depletion is most often used to study acute respiratory failure in animal models. Because model stability criticized, the authors tested following hypotheses: Repeated pulmonary lavage with normal saline provides stable experimental conditions for 24 hours a PaO2/FiO2 ratio < 300 mm Hg. Lung injury was induced by bilateral lavages 8 female pigs (51.5 +/- 4.8 kg). The animals were ventilated (PEEP: 5 cm H2O; tidal volume: 6 mL/kg; rate: 30/min). After euthanized. For histopathology...
Ischemic stroke is one of the leading causes death worldwide. It damages neurons and other supporting cellular elements in brain. However, impairment not only confined to region assault but surrounding area as well. Besides, it also brings about damage blood-brain barrier (BBB) which turn leads microvascular failure edema. Hence, this necessitates an on-going, continuous search for intervention strategies effective treatment. Of late, natural sweetener stevioside proved exhibit...
Volume therapy is a standard procedure in daily perioperative care, and there an ongoing discussion about the benefits of colloid resuscitation with hydroxyethylstarch (HES). In sepsis HES should be avoided due to higher risk for acute kidney injury (AKI). Results usage patients without are controversial. Therefore we conducted animal study evaluate impact 6% 130/0.4 on integrity or under healthy conditions Sepsis was induced by standardized Colon Ascendens Stent Peritonitis (sCASP)....
Oxidative stress occurs in remote liver injury, but the origin of oxidant generation has yet to be thoroughly delineated. Some reports suggest that source distant oxidative originates from site initial insult [i.e., xanthine oxidase (XO)]; however, it could also derived sources such as phagocytic and/or vascular NAD(P)H (Nox) enzymes. With a murine model bilateral hindlimb ischemia-reperfusion, we describe here mechanism for Nox-dependent production contributes, at least part, hepatic...
Up to 50% of septic patients develop acute kidney injury (AKI). The pathomechanism AKI is poorly understood. Therefore, we established an innovative rodent model characterize sepsis-induced by standardized colon ascendens stent peritonitis (sCASP). has a focus infection, intensive care set up with monitoring haemodynamics and oxygenation resulting in predictable impairment renal function, parameters as well histopathology scoring.Anaesthetized rats underwent the sCASP procedure, whereas sham...
Heart disease of different aetiology remains the leading cause cardiac arrest (CA). Despite efforts to improve quality cardiopulmonary resuscitation (CPR), subsequent myocardial and systemic damage after CA still present a major long-term burden. Low-dose carbon monoxide (CO) is known exert protective effects in cardiovascular pathophysiology but clinical applications are challenged by unfavourable delivery modes. We tested hypothesis that extracorporeal (E-CPR) combination with controlled...
Deleterious consequences like acute kidney injury frequently occur upon successful resuscitation from cardiac arrest. Extracorporeal life support is increasingly used to overcome high arrest mortality. Carbon monoxide (CO) an endogenous gasotransmitter, capable of reducing renal injury. In our study, we hypothesized that addition CO extracorporeal hampers severity in a porcine model Hypoxic was induced pigs. Animals were resuscitated using conventional [cardiopulmonary (CPR)], (E-CPR), or...
To investigate the impact of phophodiesterase-4 inhibition (PD-4-I) with rolipram on hepatic integrity in lipopolysaccharide (LPS) induced hyperinflammation.Liver microcirculation rats was obtained using intravital microscopy. Macrohemodynamic parameters, blood assays, and organs were harvested to determine organ function injury. Hyperinflammation by LPS PD-4-I administered intravenously one hour after application. Cell viability HepG2 cells measured EZ4U-kit based dye XTT. Experiments...