A5010455932- Traumatic Brain Injury and Neurovascular Disturbances
- S100 Proteins and Annexins
- Anesthesia and Neurotoxicity Research
- Intracranial Aneurysms: Treatment and Complications
- Cardiac Arrest and Resuscitation
- Neuroinflammation and Neurodegeneration Mechanisms
- Barrier Structure and Function Studies
- Intensive Care Unit Cognitive Disorders
- Immune Response and Inflammation
- Respiratory Support and Mechanisms
- Acute Ischemic Stroke Management
- Neurosurgical Procedures and Complications
- Neuroscience and Neuropharmacology Research
- Cardiac, Anesthesia and Surgical Outcomes
- Cancer-related molecular mechanisms research
- Traumatic Brain Injury Research
- Anesthesia and Sedative Agents
- Neurological Disease Mechanisms and Treatments
- Thermal Regulation in Medicine
- Intracerebral and Subarachnoid Hemorrhage Research
- Cerebrospinal fluid and hydrocephalus
- Mechanical Circulatory Support Devices
- Obstructive Sleep Apnea Research
- Molecular Biology Techniques and Applications
- Trauma and Emergency Care Studies
Helios Universitätsklinikum Wuppertal
2020-2025
Witten/Herdecke University
2021-2025
University Medical Center of the Johannes Gutenberg University Mainz
2015-2024
Johannes Gutenberg University Mainz
2015-2024
University of Southern Denmark
2021
Medical University of Vienna
2015
Klinik und Poliklinik für Psychosomatische Medizin und Psychotherapie
2015
Focus (Germany)
2012
Ludwig-Maximilians-Universität München
2004-2011
Leipzig University
2011
Rationale: Stroke is the third most common cause of death in industrialized countries. The main therapeutic target ischemic penumbra, potentially salvageable brain tissue that dies within first few hours after blood flow cessation. Hence, strategies to keep penumbra alive until reperfusion occurs are needed. Objective: To study effect inhaled nitric oxide on cerebral vessels and perfusion under physiological conditions different models ischemia. Methods Results: This experimental...
With a novel antibody against the rat Na(+)-D-glucose cotransporter SGLT2 (rSGLT2-Ab), which does not cross-react with rSGLT1 or rSGLT3, ∼75-kDa rSGLT2 protein was localized to brush-border membrane (BBM) of renal proximal tubule S1 and S2 segments (S1 > S2) female-dominant expression in adult rats, whereas rSglt2 mRNA similar both sexes. Castration males increased abundance protein; this increase further enhanced by estradiol prevented testosterone treatment. In BBM vesicles,...
Blood-brain barrier (BBB) integrity is one of the important elements central nervous system (CNS) homeostasis. MicroRNAs (miRs) have been demonstrated to play a role in many CNS disorders such as stroke and traumatic brain injury. MiR-212/132 are highly expressed but their at BBB has not characterized yet. Thus, we analyzed expression miR-212/132 hypoxic mouse human microvascular endothelial cells (BMEC) well posttraumatic tissue serum exosomes. was detected capillaries by situ hybridization...
Traumatic brain injury (TBI) is a major cause of death and disability. T cells were shown to infiltrate the during first days after exacerbate tissue damage. The objective this study was investigate hitherto unresolved role immunosuppressive, regulatory (Tregs) in experimental TBI."Depletion cell" (DEREG) wild type (WT) C57Bl/6 mice, treated with diphtheria toxin (DTx) deplete Tregs or serve as control, subjected controlled cortical impact (CCI) model TBI. Neurological motor deficits...
After traumatic brain injury (TBI) elderly patients suffer from higher mortality rate and worse functional outcome compared to young patients. However, experimental TBI research is primarily performed in animals. Aim of the present study was clarify whether age affects outcome, neuroinflammation secondary damage after trauma mice. Young (2 months) old (21 male C57Bl6N mice were anesthetized subjected a controlled cortical impact (CCI) on right parietal cortex. Animals both ages randomly...
Disruption of the blood-brain barrier (BBB) results in cerebral edema formation, which is a major cause for high mortality after traumatic brain injury (TBI). As anesthetic care mandatory patients suffering from severe TBI it may be important to elucidate effect different anesthetics on formation. Tight junction proteins (TJ) such as zonula occludens-1 (ZO-1) and claudin-5 (cl5) play central role BBB stability. First, influence volatile sevoflurane isoflurane in-vitro integrity was...
Ischemia, especially pericontusional ischemia, is one of the leading causes secondary brain damage after traumatic injury (TBI). So far efforts to improve cerebral blood flow (CBF) TBI were not successful because various reasons. We previously showed that nitric oxide (NO) applied by inhalation experimental ischemic stroke transported and induces vasodilatation in hypoxic regions, thus improving regional thereby neurological outcome. As ischemia penumbra a key mechanism determining...
Accumulating evidence suggests a pivotal role of PDGFRß positive cells, specific marker for central nervous system (CNS) pericytes, in tissue scarring. Identification cells that contribute to reorganization the CNS upon injury is crucial step develop novel treatment strategies regenerative medicine. It has been shown pericytes scar formation spinal cord. further known ischemia initially triggers pericyte loss vivo, whilst brain trauma capable inducing detachment from cerebral vessels. These...
Subarachnoid hemorrhage is a stroke subtype with particularly bad outcome. Recent findings suggest that constrictions of pial arterioles occurring early after may be responsible for cerebral ischemia and – subsequently unfavorable outcome subarachnoid hemorrhage. Since we recently hypothesized the lack nitric oxide cause post-hemorrhagic microvasospasms, our aim was to investigate whether inhaled oxide, treatment paradigm selectively delivering ischemic microvessels, able dilate...
To determine the neuroprotective efficacy of inert gas xenon following traumatic brain injury and to whether application has a clinically relevant therapeutic time window.Controlled animal study.University research laboratory.Male C57BL/6N mice (n = 196).Seventy-five percent xenon, 50% or 30% with 25% oxygen (balance nitrogen) treatment mechanical lesion by controlled cortical impact.Outcome trauma was measured using 1) functional neurologic outcome score, 2) histological measurement...
Although excitotoxic overactivation of glutamate receptors has been identified as a major mechanism ischemic brain damage, receptor antagonists failed in stroke trials, most cases because limited therapeutic windows or severe adverse effects. Therefore, we chose memantine and clenbuterol, both approved safe efficient their respective therapeutical categories, examined combinations these neuroprotectants for possible interactions stroke.Combinations the N-methyl-D-aspartate (NMDA) antagonist...
Quantitative measurements of gene expression require correction for tissue sample size, RNA quantity, and reverse transcription efficiency. This can be achieved by normalization with control genes. The study was designed to identify candidates not altered after brain trauma. Male C57Bl/6 mice were anesthetized isoflurane, a pneumatic trauma induced controlled cortical impact (CCI) on the right parietal cortex. Brains removed at 15 min, 3, 6, 12 24 h CCI from naive animals (n = 6 each)....
Inflammatory and ischemic processes contribute to the development of secondary brain damage after mechanical injury. Recent data suggest that thiazolidinediones (TZDs), a class drugs approved for treatment non-insulin-dependent diabetes mellitus, effectively reduces inflammation lesion by stimulation peroxisome proliferator-activated receptor-γ (PPAR-γ). The present study investigates influence TZD pioglitazone rosiglitazone on experimental traumatic injury (TBI). A controlled cortical...
<i>Background:</i> Cerebral edema is an important risk factor for death and poor outcome following subarachnoid hemorrhage (SAH). However, underlying mechanisms are still poorly understood. Matrix metalloproteinase (MMP)-9 held responsible the degradation of microvascular basal lamina proteins leading to blood-brain barrier dysfunction and, thus, formation vasogenic cerebral edema. The current study was conducted clarify role MMP-9 development functional after SAH....
HIF-1α is pivotal for cellular homeostasis in response to cerebral ischemia. Pharmacological inhibition of may reduce secondary brain damage by targeting post-translational mechanisms associated with its proteasomal degradation and nuclear translocation. This study examined the neuroprotective effects 2-methoxyestradiol (2ME2), involved HIF-1α-dependent response, alternative splicing exon 14 (HIF-1α∆Ex14) after traumatic injury (TBI) mice. Intraperitoneal 2ME2 administration 30 min TBI...
Tissue sampling for gene expression analysis is usually performed under general anesthesia. Anesthetics are known to modulate hemodynamics, receptor-mediated signaling cascades, and outcome parameters. The present study determined the influence of anesthetic paradigms typically used euthanization tissue on cerebral mRNA in mice. Naïve mice animals with acute traumatic brain injury induced by controlled cortical impact (CCI) were randomized following euthanasia protocols (n=10-11/group): no...