A5040093048- Clostridium difficile and Clostridium perfringens research
- Gut microbiota and health
- Probiotics and Fermented Foods
- Pluripotent Stem Cells Research
- Erythrocyte Function and Pathophysiology
- Zebrafish Biomedical Research Applications
- MicroRNA in disease regulation
- Pancreatic function and diabetes
- RNA Interference and Gene Delivery
- Extracellular vesicles in disease
- Platelet Disorders and Treatments
- Molecular Biology Techniques and Applications
- Cancer-related molecular mechanisms research
- Urinary Tract Infections Management
- Mesenchymal stem cell research
- Hematopoietic Stem Cell Transplantation
- Phagocytosis and Immune Regulation
- CRISPR and Genetic Engineering
- Protein Degradation and Inhibitors
- CAR-T cell therapy research
- Cytokine Signaling Pathways and Interactions
- Signaling Pathways in Disease
- Virus-based gene therapy research
- RNA modifications and cancer
- Angiogenesis and VEGF in Cancer
Beijing Radiation Center
2022-2025
Center for High Pressure Science & Technology Advanced Research
2021
United States Food and Drug Administration
2018
Abstract Thrombosis leads to platelet activation and subsequent degradation; therefore, replenishment of platelets from hematopoietic stem/progenitor cells (HSPCs) is needed maintain the physiological level circulating platelets. Platelet-derived microparticles (PMPs) are protein- RNA-containing vesicles released activated We hypothesized that factors carried by PMPs might influence production HSPCs, in a positive feedback fashion. Here we show that, during mouse acute liver injury, density...
RHOB, a member of the RHO GTPase family, is target miR-1915-3p. miR-1915-3p was proven to promote differentiation megakaryocytes while inhibiting that erythroid lineage. Although RHOB has been shown be involved in proplatelet production mice, its role early haematopoiesis, especially megakaryocytic and humans, not yet elucidated. In this study, we demonstrated function by knocking down gene progenitor cells inducing these differentiate into or cells. The silencing significantly decreased...
Blood supply shortages, especially the shortage of rare blood types, threaten current medical system. Research on stem cells has shed light in vitro cell manufacturing. The production universal red (RBCs) from induced pluripotent (iPSCs) become focus transfusion medicine. To obtain O-type Rh D-negative blood, we developed human (h)iPSCs using homology-directed repair (HDR)-based CRISPR/Cas9. HuAiPSCs derived umbilical arterial endothelial and showing haematopoietic differentiation...
Human pluripotent stem cell (hPSC)-derived red blood cells (RBCs) possess great potential for compensating shortages in transfusion medicine. For better RBC generation from hPSCs, we compared the seeding density embryoid body formation-based hPSC induction protocol. In selection of low- and high-density inoculation conditions, found that low-density culture performed final product with more output increased average cellular hemoglobin content. An elaborate study using flow cytometry...
Proper control of the lineage bias megakaryocytic and erythroid progenitor cells (MEPs) is significant importance, disorder which will lead to abnormalities in number function platelets erythrocytes. Unfortunately, signaling pathways regulating MEP differentiation largely remain be elucidated. This study aimed analyze role underlying molecular mechanism miR-1915-3p differentiation.
Abstract Background Although cord blood (CB) offers promise for treatment of patients with high-risk hematological malignancies and immune disorders, the limited numbers hematopoietic stem cell (HSC)/progenitor in a CB unit straitened circumstances expanding ex vivo make it quite challenging to develop successful therapies. Methods In this study, novel strategy has been developed support expansion progenitor cells (HSPCs) by coculture engineered human umbilical arterial endothelial...
The major obstacle for applications of human induced pluripotent stem cells (hiPSCs) is efficient and controlled lineage-specific differentiation. Hence, a deeper understanding the initial populations hiPSCs required to instruct proficient lineage commitment.hiPSCs were generated from somatic by transduction 4 transcription factors (OCT4, SOX2, KLF4, C-MYC) using Sendai virus vectors. Genome-wide DNA methylation analysis transcriptional performed evaluate capacity memory state hiPSCs. Flow...
In vitro generation of red blood cells has the potential to circumvent shortfalls in global demand for transfusion applications. However, cell differentiation and proliferation are often regulated by precise changes gene expression, but underlying mechanisms molecular remain unclear. Quantitative reverse transcription–polymerase chain reaction (qRT-PCR) can be used evaluate multiple target genes. To make results more reliable, suitable reference genes should calibrate error associated with...
Abstract Erythroblast enucleation is a precisely regulated but not clearly understood process. Polycythemia shows pathological erythroblast enucleation, and we discovered low miR-125b-5p level in terminal erythroblasts of patients with polycythemia vera (PV) compared to those healthy controls. Exogenous upregulation levels restored the rate normal levels. Direct downregulation mouse simulated issue found PV, accumulation was enucleating erythroblasts, collectively suggesting importance for...
Human pluripotent stem cell (hPSC)-derived red blood cells (RBCs) possess great potential for compensating shortages in transfusion medicine. For better RBC generation from hPSCs, we optimized the seeding density embryoid body formation-based hPSC induction protocol. In comparison of low- and high-density inoculation conditions, found that low-density culture performed final product with more output increased average cellular hemoglobin content. An elaborate study using flow cytometry...
There are long standing concerns about the use of oral antibiotics and their impact on normal gut microbiota/microbiome emergence antibiotic resistance. More recently, drug‐microbiota/microbiome interactions have been identified that impacted drug efficacy and/or safety through altered metabolism re‐enforcing these concerns. This project characterizes effect different treatment composition microbiota / microbiome prevalence antibacterial resistance genes in mice treated for UTI. knowledge...