Abstract Background N 6‐methyladenosine (m6A) modification may participate in the regulation of occurrence and development tumors. However, m6A level potential regulatory mechanism gastric cancer (GC) remain uncertain. Methods RNA quantification assay was conducted to detect GC tissues cell lines. Methyltransferase‐like 14 (METTL14) expression explored by bioinformatics immunohistochemistry. Then, function METTL14 cells examined CCK‐8, colony formation assay, wound healing Transwell assay....

The objective of this study was to investigate the expression, proliferation, and apoptosis function long-chain non-coding RNA maternally expressed gene 3 (MEG3) antisense at INK4 locus (ANRIL) in gallbladder cancer (GBC) tissues. GBC tissues adjacent normal samples were collected from 84 patients January 2008 June 2010. Empty vector, pcDNA-MEG3, pcDNA-ANRIL vectors transfected into GBC-SD QBC939 cells. An MTT assay, real-time quantitative polymerase chain reaction (RT-qPCR), flow cytometry,...

This study aims to elucidate the therapeutic efficacy and safety of a taxane-based chemotherapy in combination with immune checkpoint inhibitors regimen patients diagnosed recurrent or metastatic head neck squamous cell carcinoma (HNSCC). We retrospectively collected clinical data from 154 who received at least two cycles PD-1 as first-line treatment seven hospitals Hunan Province, between December 2018 2023. These were subjected long-term follow-up. The included eligible patients, median...

Abstract Background Gastric cancer (GC) is a common cause of cancer-related mortality worldwide, and microRNAs (miRNAs) have been shown to play an important role in GC development. This study aims explore the effect microRNA-93-5p (miR-93-5p) on epithelial-mesenchymal transition (EMT) GC, via AHNAK Wnt signaling pathway. Methods Microarray-based gene expression analysis was performed identify GC-related differentially expressed miRNAs genes. Then miR-93-5p examined tissues cell lines. The...

Autophagy, a highly conserved process of protein and organelle degradation, has emerged as critical regulator in various diseases, including cancer progression. In the context liver cancer, predictive value autophagy-related genes remains ambiguous. Leveraging chip datasets from TCGA GTEx databases, we identified 23 differentially expressed cancer. Notably, five key autophagy genes, PRKAA2, BIRC5, MAPT, IGF1, SPNS1, were highlighted potential prognostic markers, with MAPT showing significant...

Abstract Background: Esophageal cancer (EC) is one of the most common malignant tumors digestive system. MiR-25-3p was proved to be a biomarker for diagnosis and treatment many cancers. found high expressed in blood EC patients. The aim present study explore effect miR-25-3p its target gene on EC. Methods: expression patients cells detected by RT-qPCR. identified bioinformatics luciferase reporter assay. After transfection, cell viability, apoptosis, migration, invasion were MTT, flow...

The clear cell renal carcinoma (ccRCC) is the main pathological subtype of carcinoma. Immune system evasion, one hallmark cancer, contributes to cancer cells in escaping from attack immune cells. In order identify potential prognostic biomarkers ccRCC patients and fraction, we collected downloaded profiles Cancer Genome Atlas (TCGA) database Gene Expression Omnibus (GEO) database. We obtained 2 modules significantly associated with tumor stage cells; functional enrichment analysis showed...

Background The association between sex and the survival of patients with esophageal cancer (EC) remains controversial. We sought to systematically investigate sex-based disparities in EC using Surveillance, Epidemiology, End Results (SEER) registry data from United States. Methods Patients diagnosed 2004 2015 registered SEER database were selected. cancer-specific (CSS) was evaluated analysis. Inverse Probability Weighting (IPW) approach applied reduce observed bias males females. Subgroup...

Growing research has suggested an association between chronic inflammation and Intervertebral disc degeneration (IVDD), but whether there is a causal effect remains unknown. This study adopted two-sample Mendelian randomization (MR) approach to explore the etiological role of in IVDD risk. Here, summary statistics for C-reactive protein (CRP), interleukin (IL)-1

Abstract RIG‐I is associated with the occurrence and development of many tumors. However, role in radiotherapy chemotherapy NPC has not been reported to date. In our study, expression was significantly reduced chemoradiotherapy‐resistant tissues cells compared that therapy‐sensitive cells. increased nonresistant cells, including CNE1 CNE2, a dose‐dependent manner increasing drug concentration or dose. overexpression promoted sensitivity leading cellular apoptosis proapoptotic factors BAX...

Chemoradiotherapy is the standard treatment for locoregionally advanced nasopharyngeal carcinoma (NPC). We aimed to reveal factors associated with chemotherapy use and evaluate chemotherapy's benefit in patients stage III NPC stratified by lymph node status.Overall, 1452 who underwent radiotherapy (n = 1361) or without 91) were identified SEER database. examined predictors using logistic regression analysis. compared all-cause mortality (ACM) cancer-specific (CSM) Kaplan-Meier method. Cox...

Esophageal cancer (EC) is one of the most common aggressive malignant diseases worldwide. miR-28-5p plays important regulatory roles in many cancers including human EC. However, molecular mechanism and potential role EC remain uncertain. In this study, qRT-PCR western blot analysis revealed that expression was up-regulated metastasis suppressor-1 (MTSS1) down-regulated tissues relative to matched para-cancer tissues. Cell counting kit-8 (CCK-8) assay demonstrated mimics increased cell...

Anlotinib may boost the efficacy of pancreatic cancer (PC) treatment if timely added to GS regimen (Gemcitabine, Tegafur-gimeracil-oteracil potassium); however, no data has been published. This study evaluated safety and anlotinib in combination with regimen(hereafter referred as A+GS regimen) first-line patients unresectable or metastatic PC.Patients PC treated at Yueyang Central Hospital People's between October 2018 June 2022 were enrolled this retrospective real-world investigation....

Abstract Radiation therapy is the primary treatment for nasopharyngeal carcinoma (NPC). The aim of this study to identify effect Numb/Notch signaling pathway on radiation sensitivity in human NPC cells. tissues and normal were collected. To evaluate regulatory effects pathway, cells subjected radiotherapy various doses inhibitor gamma secretase (GSI). Next, expression Notch Numb proteins was determined tissues, correlation protein with clinicopathological features analyzed. Then, cell...

The aim of this study was to investigate the association between miR-1207-5p expression in peripheral blood and chemosensitivity primary gallbladder carcinoma (PGBC).A total 85 patients with PGBC undergoing preoperative chemotherapy were divided into effective (n=18) ineffective (n=67) groups. Another 70 healthy individuals selected as control group. An mimic (mimic group), an inhibitor (inhibitor a negative (NC group) sequence transfected human GBC-SD cells. Real-time quantitative...

Abstract Introduction: The treatment strategy for low-grade gliomas (LGGs) is still controversial, and there are no standardized criteria to predict the prognosis of patients with LGGs. Magnetic resonance imaging (MRI) a routine test preoperative diagnosis LGG can reflect destructive features tumor. In present study, we aimed explore relationship between MRI in LGG. Methods: Clinical data 80 pathologically proved LGGs January 2010 December 2016 were analyzed retrospectively. classified as...

<title>Abstract</title> Growing research has suggested an association between chronic inflammation and Intervertebral disc degeneration (IVDD),but whether there is a causal effect remains unknown. This study adopted two-sample Mendelian randomization (MR) approachto explore the etiological role of in IVDD risk. Here, summary statistics for C-reactive protein (CRP),interleukin (IL)-1α, IL-1β, IL-6 expression were obtained from genome-wide studies (GWAS) Europeanancestry. MR analyses conducted...

Subtrochanteric femur fracture (SFF) is one of the more common diseases in traumatological orthopaedics, with two types prevalent populations: young people high-energy injuries and elderly low-energy injuries. Due to special anatomical location subtalar femoral region, perioperative complications, complex underlying patients, SFF has become very challenging clinical treatment. With gradual attention many scholars this disease continuous improvement surgical techniques, there are methods for...

Background Few real-world studies exist regarding the clinical value of local consolidative therapy (LCT) for oligo-residual disease (ORD) in NSCLC patients treated with immune checkpoint inhibitors. Therefore, we retrospectively evaluated whether LCT could improve prognosis NSCL ORD after effective first-line PD-1/PD-L1 inhibitors treatment. Methods A total 132 metastatic who had received PD-1/PD-L1inhibitors-based systemic treatment and developed (defined as residual tumors limited to...

Biliary tract cancer (BTC) is the second common in liver cancer. Chemotherapy mainstay of treatments for patients with advanced or metastatic disease, while fluorouracil (FU)-based and gemcitabine (GEM)-based are most widely applied. This NMA aimed to figure out whether addition platinum (PLA) target agents (TAR) can influence efficacy safety standard chemotherapy. Network meta-analysis (NMA) was conducted based on records from PubMed, Embase Cochrane. Eligible data extracted available...