A5103180520- Neuroscience and Neuropharmacology Research
- Photoreceptor and optogenetics research
- Neurotransmitter Receptor Influence on Behavior
- Receptor Mechanisms and Signaling
- Neuroscience and Neural Engineering
- Conducting polymers and applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Photochromic and Fluorescence Chemistry
- Neurogenesis and neuroplasticity mechanisms
- Photoacoustic and Ultrasonic Imaging
- Advanced Fluorescence Microscopy Techniques
- Neural dynamics and brain function
- Tryptophan and brain disorders
Columbia University
2013-2020
Columbia University Irving Medical Center
2017
To address existing limitations in live neuron imaging, we have developed NeuO, a novel cell-permeable fluorescent probe with an unprecedented ability to label and image neurons selectively over other cells the brain. NeuO enables robust imaging isolation vivo vitro across species; its versatility ease of use sets basis for development myriad neuronal targeting applications.
Abstract Norepinephrine is a monoamine neurotransmitter with wide repertoire of physiological roles in the peripheral and central nervous systems. There are, however, no experimental means to study functional properties individual noradrenergic synapses brain. Development new approaches for imaging synaptic neurotransmission fundamental importance specific changes that occur during learning, behavior, pathological processes. Here, we introduce fluorescent false 270 (FFN270), tracer...
Ongoing efforts in our laboratories focus on design of optical reporters known as fluorescent false neurotransmitters (FFNs) that enable the visualization uptake into, packaging within, and release from individual monoaminergic neurons presynaptic sites brain. Here, we introduce molecular probe FFN246 an expansion FFN platform to serotonergic system. Combining acridone fluorophore with ethylamine recognition element serotonin, identified FFN54 substrates for both serotonin transporter...
Voltage sensitive fluorescent dyes (VSDs) are important tools for probing signal transduction in neurons and other excitable cells. The impact of these highly lipophilic sensors has, however, been limited due to the lack cell-specific targeting methods brain tissue or living animals. We address this key challenge by introducing a nongenetic molecular platform cell- molecule-specific synthetic VSDs brain. employ dextran polymer particle overcome inherent lipophilicity dynamic encapsulation...
To advance understanding of the brain, ability to measure both nerve cell electrical spiking and chemical neurotransmission with high spatial resolution is required. In comparison development voltage sensors Ca(2+) indicator dyes over past several decades, imaging neurotransmitter (NT) release at single synapses has not been possible. this Viewpoint, we discuss two recent developments toward goal, namely, design fluorescent false neurotransmitters (FFNs) optical sensors.
We have previously introduced fluorescent false neurotransmitters (FFNs) as optical reporters that enable visualization of individual dopaminergic presynaptic terminals and their activity in the brain. In this context, we examined pyridinium dye 4-(4-dimethylamino)phenyl-1-methylpyridinium (APP+), a analogue neurotoxin MPP+, acute mouse brain tissue. APP+ is substrate for dopamine transporter (DAT), norepinephrine (NET), serotonin (SERT), such represented candidate development new FFN...
Abstract To address existing limitations in live neuron imaging, we have developed NeuO , a novel cell‐permeable fluorescent probe with an unprecedented ability to label and image neurons selectively over other cells the brain. enables robust imaging isolation vivo vitro across species; its versatility ease of use sets basis for development myriad neuronal targeting applications.
Few tools are available for noninvasive imaging of synapses in the living mammalian brain. Current paradigms require use genetically modified mice or viral delivery genetic material to To develop an alternative chemical approach, utilizing recognition synaptic components by organic small molecules, we designed imaging-based, high-content screen cultured cortical neurons identify molecules based on their colocalization with fluorescently tagged proteins. We used this approach a library ∼7000...
Voltage sensitive fluorescent dyes (VSDs) are important tools for probing signal transduction in neurons and other excitable cells. These sensors, rendered highly lipophilic to anchor the conjugated p-wire molecular framework membrane, offer several favorable functional parameters including fast response kinetics high sensitivity membrane potential changes. The impact of VSDs has however been limited due lack cell-specific targeting methods brain tissue or living animals. We address this key...