A5002075588- Pharmacogenetics and Drug Metabolism
- Drug Transport and Resistance Mechanisms
- Drug-Induced Adverse Reactions
- Estrogen and related hormone effects
- Hormonal Regulation and Hypertension
- Contact Dermatitis and Allergies
- Autoimmune Bullous Skin Diseases
- Identification and Quantification in Food
- Computational Drug Discovery Methods
- Urticaria and Related Conditions
- Hormonal and reproductive studies
- Ovarian cancer diagnosis and treatment
- Metabolism and Genetic Disorders
- Glycosylation and Glycoproteins Research
- Eicosanoids and Hypertension Pharmacology
- Neonatal Health and Biochemistry
- Epigenetics and DNA Methylation
- Trace Elements in Health
- Pharmacological Effects and Toxicity Studies
- Pneumocystis jirovecii pneumonia detection and treatment
- Endometrial and Cervical Cancer Treatments
- Monoclonal and Polyclonal Antibodies Research
- Genomics, phytochemicals, and oxidative stress
- Genetic factors in colorectal cancer
- Biosensors and Analytical Detection
Tokyo University of Marine Science and Technology
2014-2024
National Institute of Health Sciences
2007-2016
Nagoya City University
2015
Mitsubishi Tanabe Pharma Corporation
2015
Nippon Medical School
2001-2011
PharmacoGenetics (China)
2002-2009
Chiba Hokusou Hospital
2006
University of Manitoba
2001
Cancer Genetics (United States)
2001
Health Sciences Centre
2001
Introduction: Stevens–Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN) are rare but life-threatening severe cutaneous adverse reactions. Recently, strong associations of HLA-B*1502 HLA-B*5801 with carbamazepine- allopurinol-induced reactions were found in Han Chinese patients, respectively, ethnic differences the have been reported. The objective this study is to clarify involvement Japanese SJS/TEN patients. Methods: HLA-B genotyping was performed on 58 patients between July 2006...
Summary Stevens‐Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN) are rare but life‐threatening severe cutaneous adverse reactions. Recently, strong associations of HLA‐B*1502 with carbamazepine‐induced SJS/TEN have been found in Han Chinese patients. These confirmed several Asian populations, excluding Japanese. SJS patients carrying HLA‐B*1508, HLA‐B*1511, or HLA‐B*1521, which members the HLA‐B75 type along HLA‐B*1502, were detected studies India Thailand. In current study, we...
Although numerous studies have reported that high frequencies of loss heterozygosity (LOH) at various chromosomal arms been identified in breast cancer, differential LOH the neoplastic epithelial and surrounding stromal compartments has not well examined. Using laser capture microdissection, which enables separation epithelium from stroma, we microdissected each compartment 41 sporadic invasive adenocarcinomas breast. Frequent was both and/or compartments, ranging 25 to 69% cells, 17 61%...
In order to identify single nucleotide polymorphisms (SNPs) and haplotype frequencies of CYP3A4 in a Japanese population, the distal enhancer proximal promoter regions, all exons, surrounding introns were sequenced from genomic DNA 416 subjects. We found 24 SNPs, including 17 novel ones: two enhancer, four promoter, one 5′-untranslated region (UTR), seven introns, three 3′-UTR. The most common SNP was c.1026+12G>A (IVS10+12G>A), with 0.249 frequency. Four non-synonymous c.554C>G (p.T185S,...
Ethnic differences in genetic polymorphisms UDP-glucuronosyltransferase 1A1 (<i>UGT1A1</i>) were investigated among African-Americans, Caucasians, and Japanese using samples obtained from 150 individuals for each population. Genotyping of –3279T>G the phenobarbital-responsive enhancer module, TA repeats TATA box, 211G>A (G71R) 686C>A (P229Q) exon 1, three single nucleotide (SNPs) (1813C> T, 1941C>G, 2042C>G) 3′-untranslated region 5 was performed. Eight haplotypes block 1...
L1 (LINE-1) is a long interspersed repetitive sequence derived from retrotransposon. Transfection studies using the CAT gene as reporter demonstrated that first 155bp in human contains an element(s) responsible for promoter activity HeLa cells. The transcription was shown to initiate at nucleotide of transgene. Three prominent nuclear protein binding sites were found 5' region by DNaseI footprint analysis. One sites, designated site A located +3 +26, be essential because mutation caused...
Metabolism of administered drugs is determined by expression and activity many drug-metabolizing enzymes, such as the cytochrome P450 (P450s) family members. Pregnane X receptor (PXR) a master transcriptional regulator drug/xenobiotic-metabolizing including P450s drug transporters. In this study, we describe functional analysis four naturally occurring human PXR (hPXR) variants (R98C, R148Q, R381W, I403V) that have recently identified. By reporter gene assay using <i>CYP3A4</i>...
Aim: This preliminary study investigated genomic biomarkers for Stevens–Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN), related to three antiepileptic drugs, zonisamide, phenobarbital phenytoin. Patients & methods:HLA class I HLA-DRB1 loci were genotyped Japanese patients with zonisamide-, phenobarbital- or phenytoin-induced SJS/TEN (n = 12, 8 9, respectively) healthy volunteers 2878). Results: Carrier frequencies of HLA-A*02:07 in zonisamide-induced the general population 41.7...
The small intestine plays an important role in all aspects of pharmacokinetics, but there is no system for the comprehensive evaluation small-intestinal including drug metabolism and absorption. In this study, we aimed to construct intestinal pharmacokinetics generate pharmacokinetically functional enterocytes from human induced pluripotent stem cells. Using activin A fibroblast growth factor 2, differentiated these cells into cell–like cells, resulting were a medium containing epidermal...
In this study, we aimed to elucidate the effects and mechanism of action valproic acid on hepatic differentiation from human induced pluripotent stem cell-derived progenitor cells. Human cells were differentiated into endodermal in presence activin A then using dimethyl sulfoxide. Hepatic matured hepatocyte growth factor, oncostatin M, dexamethasone with that was added during maturation process. After 25 days differentiation, expressed marker genes drug-metabolizing enzymes exhibited enzyme...
Aequorin is a monomeric Ca2+-binding protein (Mr, 21,400) that emits light upon reacting with Ca2+. The has three sites, cysteine residues, and noncovalently bound chromophore consists of coelenterazine molecular oxygen. Light emitted via an intramolecular reaction in which oxidized by the After emission, aequorin may be regenerated incubating coelenterazine, dissolved oxygen, EDTA, 2-mercaptoethanol. To understand structure-function relationships this protein, we used technique...
Glucuronidation, catalyzed by UDP-glucuronosyltransferases (UGTs), is important in the detoxification and enhanced elimination of a large number endogenous exogenous substrates. The human UGT1A gene complex contains at least nine variations exon 1, common exons 2–5, single 1 splices to 2–5 (1). Of isoforms, UGT1A1 primarily responsible for glucuronidation bilirubin liver can also conjugate phenols, anthraquinones, flavonoids, variety therapeutic drugs their metabolites (e.g., SN-38, an...
Journal Article RNA polymerase III dependence of the human L1 promoter and possible participation II factor YY1 in transcription system Get access Kouichi Kurose, Kurose Human Genome Center, The Institute Medical Science, University Tokyo4-6-1 Shirokanedai, Minato-ku, Tokyo 108, Japan Search for other works by this author on: Oxford Academic PubMed Google Scholar Kikumi Hata, Hata Masahira Hattori, Hattori Yoshiyuki Sakaki * *To whom correspondence should be addressed Nucleic Acids Research,...