A5044963534- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Infective Endocarditis Diagnosis and Management
- Mycobacterium research and diagnosis
- Urinary Tract Infections Management
- Ocular Diseases and Behçet’s Syndrome
- Cytomegalovirus and herpesvirus research
- Immune Response and Inflammation
- Streptococcal Infections and Treatments
- Antibiotic Resistance in Bacteria
- Hepatitis C virus research
- Surgical site infection prevention
- Herpesvirus Infections and Treatments
- Nosocomial Infections in ICU
- Antibiotic Use and Resistance
- Viral-associated cancers and disorders
- Polyomavirus and related diseases
- Hepatitis B Virus Studies
- Monoclonal and Polyclonal Antibodies Research
- Escherichia coli research studies
- Colorectal Cancer Surgical Treatments
- Toxoplasma gondii Research Studies
- Infection Control in Healthcare
- Liver Disease Diagnosis and Treatment
- Infectious Diseases and Mycology
Meander Medisch Centrum
2015-2025
Saxion University of Applied Sciences
2024
Rijnstate Hospital
2018
National Institute for Public Health and the Environment
2011
University Medical Center Utrecht
1997-2007
University Hospital Heidelberg
1997-2002
Heidelberg University
1997-2002
Eijkman Institute for Molecular Biology
2000
Dutch Health Care Inspectorate
2000
Utrecht University
1997-1999
Abstract The absolute number of membrane-expressed CD14, the most important endotoxin receptor, on human monocytes and neutrophils shows remarkable variation in literature. To quantify these numbers two fluorescence methods using fluorescein isothiocyanate (FITC)-labeled monoclonal antibodies (mAb) were applied. A commercially available set standard beads was used flow cytometry to quantitate CD14 with eight different mAbs. Independent from their isotype various mAbs showed minor differences...
In order to determine the prevalence of vancomycin-resistant enterococci (VRE) in The Netherlands, 624 hospitalized patients from intensive care units or hemato-oncology wards nine hospitals and 200 living community were screened for VRE colonization. Enterococci found 49% 80% community. Of these strains, 43 32%, respectively, Enterococcus faecium. isolated 12 (2%) 4 community, respectively. PCR analysis 16 strains 11 additional clinical isolates one participating revealed 24 vanA...
The benefit of screening healthcare workers (HCWs) at risk for methicillin-resistant Staphylococcus aureus (MRSA) carriage and furloughing MRSA-positive HCWs to prevent spread patients is controversial. We evaluated our MRSA program between 1992 2002.A university medical center in Netherlands, where methicillin resistance has been kept below 0.5% all nosocomial S. infections using active surveillance cultures isolation colonized patients.HCWs caring or foreign hospitals were screened MRSA....
Abstract Objective: Hospital-onset bacteremia and fungemia (HOB) has been suggested as a suitable automatable surveillance target to include in programs, however differences definitions across studies limit interpretation large-scale implementation. We aimed apply an automated system for HOB multiple hospitals using consensus definition, describe rates. Design setting: Retrospective cohort study four Dutch hospitals: 1 tertiary hospital 3 secondary hospitals. Patients: All patients admitted...
Several LPS-binding proteins have been identified on the surface of human granulocytes (polymorphonuclear leukocyte (PMN)). We describe a plasma-membrane associated ca. 55-kDa protein PMN that is indistinguishable from bactericidal/permeability-increasing (BPI). To detect cell surface, were biotinylated before detergent solubilization and incubation with LPS-coated beads. bound to beads but not uncoated characterized after elution by SDS-PAGE western blotting using streptavidin-horseradish...
The Netherlands is one of the six European countries considered on track to eliminate hepatitis C virus by 2030. To achieve this goal, continuous efforts have be put into designing efficient case-finding strategies, including retrieval previously diagnosed virus-infected who are lost follow-up.To trace and treat all follow-up patients in Utrecht region create an strategy that can used future (national) initiatives.Positive diagnostic tests (anti-hepatitis IgG or virus-RNA) from laboratory...
We used rough lipopolysaccharide (ReLPS) to construct a fluorescein-labeled LPS (FITC-LPS) with very high labeling efficiency that bound isolated human monocytes in CD14-dependent fashion and this respect behaved indistinctively from native LPS. The binding could be inhibited either by 1,000-fold excess of unlabeled or polymyxin B, bactericidal/permeability-increasing protein, cationic protein 18, soluble CD14. Although FITC-LPS preparation no longer possessed the ability prime neutrophils...
We analyzed the effect of cytomegalovirus (CMV) serostatus on overall survival (OS) and transplant-related mortality (TRM) in 253 consecutively treated patients receiving partially T cell-depleted (TCD) bone marrow from either matched related donors (MRDs; n=205) or unrelated (MUDs; n=48). Short-course, low-dose preemptive therapy with ganciclovir was provided as soon a positive antigenemia assay result obtained. Ganciclovir prophylaxis, which identical to therapy, given acute...
Candida auris is a rapidly emerging multidrug-resistant pathogenic yeast. In recent years, an increasing number of C. invasive infections and colonized patients have been reported, has associated with hospital outbreaks worldwide, mainly in intensive care units (ICUs). Here, we describe the first two cases The Netherlands. Both were treated healthcare facility India prior to admission. routinely placed contact precautions single room after admission, which common practice Netherlands for...
ObjectiveShort-course aminoglycosides as adjunctive empirical therapy to β-lactams in patients with a clinical suspicion of sepsis are used broaden antibiotic susceptibility coverage and enhance bacterial killing. We quantified the impact this approach on 30-day mortality subset Gram-negative bloodstream infection.MethodsFrom prospective cohort study conducted seven hospitals Netherlands between June 2013 November 2015, we selected all infection (GN-BSI). Short-course aminoglycoside was...
Abstract Objectives To determine trends, seasonality and the association between community antibiotic use antimicrobial resistance (AMR) in Escherichia coli Klebsiella pneumoniae urinary tract infections. Methods We analysed Dutch national databases from January 2008 to December 2016 regarding AMR for nitrofurantoin, trimethoprim, fosfomycin ciprofloxacin. Antibiotic was expressed as DDD/1000 inhabitant-days (DID) percentage of total tested isolates. Temporal trends were with autoregressive...
Objective: To determine the prevalence and determinants of fecal carriage vancomycin-resistant enterococci (VRE) in intensive care unit (ICU), hematology-oncology, hemodialysis patients The Netherlands. Design: Descriptive, multicenter study, with yearly 1-week point-prevalence assessments between 1995 1998. Population: All hospitalized on testing days ICUs hematology-oncology wards nine hospitals Netherlands were included. Methods: Rectal swabs obtained from 1,112 screened for a selective...
Antibiotic resistance in Gram-negative bacteria has been associated with increased mortality. This was demonstrated mostly for third-generation cephalosporin-resistant (3GC-R) Enterobacterales bacteraemia international studies. Yet, the burden of specifically Netherlands and created by all types infection not quantified. We therefore investigated attributable mortality antibiotic infections Netherlands.
The binding of rough LPS (ReLPS from Salmonella minnesota R595) to human peripheral blood polymorphonuclear leukocytes (PMN), monocytes, and lymphocytes was examined by using fluorescein-labeled flow cytometry. At 4 degrees C, FITC-ReLPS bound rapidly in a concentration- time-dependent way PMN, lymphocytes. Because mononuclear cells showed both nonbinding cell populations, used conjunction with specific phycoerythrin-labeled mAb identify these subpopulations. In contrast T NK cells, all...
ABSTRACT Among Nocardia species causing infections, veterana is rarely isolated and mostly described as pulmonary infections. This the first presentation of a case brain abscess attributable to an N. infection in patient with type 2 diabetes. Prolonged antibiotic therapy trimethoprim-sulfamethoxazole led successful clinical recovery.
Direct health effects of antibiotic resistance are difficult to assess. We quantified the risk recurrent bacteremia associated with resistance.We extracted antimicrobial susceptibility testing data on blood isolates from Dutch surveillance system for between 2008 and 2017. First first (4-30 days) episodes were categorized as susceptible, single nonsusceptible, or co-nonsusceptible third-generation cephalosporins without carbapenems (Enterobacteriaceae), ceftazidime (Pseudomonas species),...