A5101478233- Pregnancy and preeclampsia studies
- Reproductive System and Pregnancy
- MicroRNA in disease regulation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Systemic Lupus Erythematosus Research
- Maternal and fetal healthcare
- Cell Adhesion Molecules Research
- Nitric Oxide and Endothelin Effects
- Birth, Development, and Health
- Preterm Birth and Chorioamnionitis
- Extracellular vesicles in disease
- Platelet Disorders and Treatments
- COVID-19 Impact on Reproduction
- Cancer-related molecular mechanisms research
- Renal Diseases and Glomerulopathies
- Advanced Glycation End Products research
- Pregnancy and Medication Impact
- COVID-19 and healthcare impacts
- Epigenetics and DNA Methylation
- Endometriosis Research and Treatment
- Autophagy in Disease and Therapy
- Circular RNAs in diseases
- Blood disorders and treatments
- Fibroblast Growth Factor Research
- Endoplasmic Reticulum Stress and Disease
National Health and Family Planning Commission
2019-2024
Shandong University
2015-2024
Shandong First Medical University
2021-2024
Qingdao University
2024
Shandong Provincial Hospital
2015-2024
Shandong Maternal and Child Health Hospital
2019-2022
Wenzhou Medical University
2020
Abstract Despite the widespread use of antiplatelets and anticoagulants, women with antiphospholipid syndrome (APS) may face pregnancy complications associated placental dysplasia. Neutrophil extracellular traps (NETs) are involved in pathogenesis many autoimmune diseases, including vascular APS; however, their role obstetric APS is unclear. Herein, we investigated NETs by quantifying cell‐free DNA NET marker levels. Live‐cell imaging was used to visualize formation, MAPK signalling pathway...
Abstract Obstetric antiphospholipid syndrome (OAPS) is mediated by antibodies (aPLs, and anti‐β2 glycoprotein I antibody the main pathogenic antibody), recurrent abortion, preeclampsia, foetal growth restriction other placental diseases are clinical characteristics of pathological pregnancy. It a disease that seriously threatens health pregnant women. Hydroxychloroquine (HCQ) was originally used as an anti‐malaria drug has now shown benefit in refractory OAPS where conventional treatment...
The abnormal expression of long non-coding RNAs (LncRNAs) in platelet-derived microparticles (PMPs) is closely related to immune disorders and may lead antiphospholipid antibody syndrome recurrent miscarriage. To understand the association between LncRNAs PMPs RM/APS, differences RM/APS patients healthy controls were analyzed. Microarray analysis RT-qPCR detection proved that patient exhibited high levels LncNR_040117 expression. lentiviral silent transfection HTR-8/SVneo cells indicated...
Platelet-derived microparticles (PMPs) are a type of microparticle budding from platelets undergoing activation or apoptosis in many autoimmune diseases, including antiphospholipid antibody syndrome (APS). PMPs may also contribute to recurrent miscarriage, although the exact mechanism is unclear. The aim this study was determine potential biological by which abnormal PMP affect miscarriage. were counted fluorescence-activated cell sorting (FACS) and compared between healthy control (HC)...
Recent studies suggest that autophagy is highly involved in insulin resistance (IR). Inhibition of the PI3K/AKT/mTOR signaling pathway induces activation. Additionally, depletion LRP6 has been shown to increase sensitivity but its mechanism still not clear. We hypothesized contributes IR by regulating mTOR mediated through GSK3β hepatocytes. LO2 hepatocytes were treated with palmitate (PA) and induced IR. Levels mRNA protein expression measured real time-PCR western blot analysis. knock down...
Background Antiphospholipid syndrome (APS) is an autoimmune disease characterized by thrombotic events and/or pregnancy morbidity (≥3 recurrent early miscarriage or fetal death a prematurity <34 weeks of gestation) with persistently positive antiphospholipid antibodies (aPLs). It reported that aPLs damage the placental tissue binding to β2-glycoprotein I (β2GPI) on surface trophoblast and endothelial cells. Hydroxychloroquine (HCQ) considered be beneficial in treatment obstetrical APS shown...
Decorin (DCN) regulates a vast array of cellular processes including proliferation, migration, apoptosis, and autophagy, its aberrant expression has been associated with poor extravillous trophoblasts (EVT) invasion the uterus, which underlies occurrence preeclampsia (PE) intrauterine growth restriction (IUGR). In this study, we aim to elucidate molecular mechanism how DCN cell functions through use trophoblast line, HTR-8. Using series function assays, CCK8, RTCA, transwell, scratch-wound...
Objective: To evaluate the association between preeclampsia and three single nucleotide polymorphisms (rs13405728 in LHCGR gene; rs13429458 THADA gene, rs2479106 DENND1A gene) which were identified to be genetic variants of polycystic ovary syndrome (PCOS) by genome-wide study Han Chinese populations. Methods: A total 784 northern women (378 controls 406 cases) genotyped for polymerase chain reaction direct sequencing. Unconditional logistic regression analysis was used adjust impact...
Preeclampsia (PE) is a major cause of maternal and perinatal morbidity mortality. Studies on the role microRNAs (miRNAs), in pathogenesis PE through their effects trophoblast function have been reported, but roles for some miRNAs including miR-513c-5p, not identified. We aimed to evaluate potential miRNA candidates that regulate LRP6 mRNAand elucidate possible mechanism PE. Potential were selected by bioinformatics analysis, PCR placenta tissues dual luciferase reporter assay HTR-8/SVneo...
Amniotic fluid exosomes (AF-Exos) from term labor (TL) cause amniotic membrane senescence and induce labor. However, the intrinsic mechanism through which this occurs remains unknown. Therefore, we performed microRNA (miRNA) microarray chip screening of AF-Exos obtained TL terms not in discovered that expression miR-548az-5p was significantly upregulated TL. This study aimed to explore role AF-Exos-induced human epithelial cells (hAECs) for initiation. Bioinformatics analysis revealed...
Inflammatory stress at the maternal-fetal interface plays an important role in occurrence and development of preeclampsia(PE) caused by different etiologies. Many pathological neutrophil extracellular traps (NETs) are believed to be among main pathogenic factors leading preeclampsia worsening its symptoms. However, underlying mechanism is largely unclear. This study aimed elucidate high mobility group box 1 (HMGB1) NETs involved pathogenesis PE. The concentration was detected plasma patients...
Objective: To detect the role of endothelial progenitor cells (EPCs) treated with platelet microparticles (PMPs) in preeclampsia.Methods: EPCs with/without PMPs were labeled and injected to PE rats. The differentiation EPCs, change nitric oxide synthase (eNOS), blood pressure proteinuria measured. increased each groups, improved by which was strengthened PMPs. Transplantation placental angiogenesis. trend NO same as pressure.Conclusion: more effectively.
The primary objective of this study was to explore whether antisense oligonucleotides (ASOs) that reduce LncNR_040117 expression in patients with antiphospholipid antibody syndrome (APS)-induced recurrent pregnancy loss (RPL), and further decrease apoptosis improve trophoblasts invasion through mitogen-activated protein kinase (MAPK) pathways. This paper aimed provide a new strategy treat APS-induced RPL.In study, we used quantitative reverse transcription-polymerase chain reaction (RT-qPCR)...
The study aims to analyze the pregnancy outcomes of multiple gestations with preterm premature rupture membranes (PPROM) that occurred within 24 h after fetal reduction potassium chloride (KCL). We identified and evaluated 16 retrospectively recorded multigestational pregnancies met inclusion criteria between 2006 2016, from Obstetrics Department Shandong Provincial Hospital. A total patients carrying twins or higher order experienced PPROM reduction, all them received expectant management...