Abstract Background Patients with diabetes have an increased risk of nonunion and delayed union fractures. Macrophages been shown as a key player in diabetic complications. However, it remains obscure how milieu affects macrophage-derived exosomes its implications on osteogenic differentiation BMSCs. In this study, we aim to define the impact exosomes, role extracellular vesicles intercellular communication BMSCs, subsequent effects fracture repair. Results The potential ability repair...

Altered expression of epithelial or stromal caveolin-1 (Cav-1) is observed in various types human cancers. However, the clinical significance Cav-1 gastric cancer (GC) remains largely unknown. The present study aims to explore clinicopathological and prognostic value both tumor cells associated fibroblasts (CAFs) GC.Quantum dots immunofluorescence histochemistry was performed examine 20 cases gastritis without intestinal metaplasia (IM), with IM 286 GC. Positive rates IM, GC showed a...

Angiogenesis is involved in the wound healing process. Increased angiogenesis and blood flow constitute a major mechanism of negative pressure therapy (NPWT), which has been shown to facilitate infected wounds. However, effect on expression angiogensis‑related growth factor remains unknown. The goal current study was investigate angiogenic levels prior following NPWT A total 20 patients with wounds treated were included study. Patients acted as their own control; postoperative measurements...

Abstract The use of fibular graft for the reconstruction bone defects has been demonstrated to be a reliable method. aim this study was assess clinical outcome union, functional (hypertrophy bones) and complications both non-vascularized vascularized grafts. From 1981 2015, 10 patients were treated using or free graft. outcomes bony union time, hypertrophy based on radiograph according Musculoskeletal Tumor Society (MSTS) score. Mobility ankle at donor site evaluated Kofoed score system....

Negative pressure wound therapy (NPWT) has been demonstrated to accelerate healing by promoting angiogenesis. However, whether blood flow perfusion is regulated microvessel maturation and pericytes following NPWT remains unclear, as well the exact association between collagen type IV. The aim of this study was investigate relevant NPWT, further explore underlying molecular mechanisms. We also aimed For purpose, we created a rat model diabetic wounds microvascular detected using laser Doppler...

Negative pressure wound therapy (NPWT) has been observed to accelerate the healing process in humans through promoting angiogenesis. However, potential biological effect and relevant molecular mechanisms, including microvessel destabilization, regression endothelial cell proliferation early stage (1-3 days), neovascular stabilization maturation later (7-15 have yet be fully elucidated. The current study aimed research of NPWT on angiogenesis vessel maturation, investigate association between...

This meta-analysis was performed to evaluate the efficiency and safety of absorbable implants. Five major electronic databases (PubMed, Embase, Cochrane Library, SinoMed Wanfang Data) were systematically searched for randomized controlled trials (RCTs) from their establishment November 2012. Studies on implants metal ankle fractures selected. The using RevMan 5.1. Ten studies with 762 patients included analyzed. Compared implants, used internal fixation produce similar radiographic...

Negative-pressure wound therapy (NPWT) is an effective way to promote healing. However, its mechanisms have not been investigated thoroughly. Growing evidence suggests that oxidative stress and Raftlin levels play important roles in whether NPWT promotes healing through this mechanism remains unclear.Our study focuses on the different of antioxidant response between wounds treated by routine dressing change. The objective was measure differences two groups, which a new biomarker related...

Traumatic heterotopic ossification (HO) represents an intractable sequela following trauma with no currently effective prophylaxis or treatment. Photodynamic therapy (PDT) is a non-invasive treatment for various proliferative diseases. However, the specific effects of PDT on HO development remain unclear. In this study, therapeutic potential near-infrared (NIR) probe-WL-808, composed type II collagen-binding peptide (WYRGRL) and photosensitizer (IR-808), was evaluated innovative HO-targeted...

Severe traumatic wounds are challenging to manage during surgery. The introduction of vacuum-assisted closure (VAC) is a breakthrough in wound management. aim the present study was investigate effect VAC on cytokines management severe following initial debridement. and conventional care (CWC) were independently applied pigs. expression levels intercellular adhesion molecule-1 (ICAM-1), migration inhibitory factor (MIF), vascular endothelial growth (VEGF), basic fibroblast factor, collagen I...

The aim of this meta-analysis was to determine the efficacy corticosteroid versus placebo injection for treatment plantar fasciitis. Databases (Medline, Embase, Cochrane Library and Google Scholar) study references were searched randomized controlled trials comparing with Studies that met inclusion criteria selected analysis. risk bias tool used methodological assessment. Outcomes including visual analogue score (VAS) fascia thickness (PFT) extracted pooled. Egger's test detect publication...

Vacuum-assisted closure (VAC) negative pressure wound therapy (NPWT) has been proven to be an effective therapeutic method for the treatment of recalcitrant wounds. However, its role in bone healing remains unclear. Here, we investigated effects NPWT on rat periosteum-derived mesenchymal stem cells (P-MSCs) proliferation and osteoblastic differentiation a 3D fibrin matrix. P-MSCs underwent primary culture three passages before being used construct cell clots. The clots were incubated with...

Background: Negative pressure wound therapy represents an effective to treat nonhealing diabetic wounds by promoting angiogenesis, of which the mechanism hasn't been investigated thoroughly. Growing evidence suggests that miRNAs hold great potential be clinical biomarkers, and miR-126 is essential angiogenesis regulator in repair. Purpose: Our study aims explore effect NPWT on expression tissue plasma rat models association between circulating two quantitative indexes angiogenesis. Methods:...

Traumatic heterotopic ossification (THO) is a devastating sequela following traumatic injuries and orthopedic surgeries. To date, the exact molecular mechanism of THO formation still unclear, which hinders development effective treatments. The process believed to recapitulate series spatiotemporal cellular signaling events that occur during skeletal development. Notch pathway critical genetic regulator in embryological bone fracture healing. However, few data are available concerning whether...

Aims and background Hypoxia is a fundamental microenvironmental component of osteosarcoma which induces activation the hypoxia-inducible factor-1 (HIF-1) pathway. Overexpression HIF-1α has been linked to tumor resistance radio- or chemotherapy. However, little known about effects inhibition on hypoxic radioresistance human cells. Here, we investigated cell survival radiosensitivity in MG-63 line. Methods was achieved by small interfering RNA (siRNA) targeting via chetomin. Inhibition HIF-1...

The aim of this study was to investigate the efficiency negative pressure wound therapy (NPWT) combined with open bone graft (OBG; NPWT-OBG) for treatment and soft tissue defects polluted wounds in an animal model. All rabbits were randomly divided into two groups, experimental group (NPWT graft) control (OBG). efficacy assessed by conditions healing time. Bacterial bioburdens bony calluses evaluated bacteria counting X-rays, respectively. Furthermore, granulation samples from on days 0, 3,...

Traumatic heterotopic ossification (HO) is a devastating sequela of orthopedic surgeries and traumatic injuries; however, few studies have explored the effects estrogen-deficient state on HO formation. In present study, we investigated impact estrogen deficiency ectopic cartilage bone formation in tendon after Achilles tenotomy an ovariectomized mouse model.