A5100374344- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- Cancer Mechanisms and Therapy
- Pediatric Hepatobiliary Diseases and Treatments
- Histone Deacetylase Inhibitors Research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Viral Infectious Diseases and Gene Expression in Insects
- RNA Interference and Gene Delivery
- Chromatin Remodeling and Cancer
- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Schizophrenia research and treatment
- Mental Health Research Topics
- Cancer-related Molecular Pathways
- Bioactive natural compounds
- Neurological diseases and metabolism
- Seed and Plant Biochemistry
- Circular RNAs in diseases
- Bladder and Urothelial Cancer Treatments
- Inflammasome and immune disorders
- Bone Metabolism and Diseases
- Extracellular vesicles in disease
- Liver Disease Diagnosis and Treatment
- Mechanisms of cancer metastasis
West China Hospital of Sichuan University
2019-2025
Sichuan University
2019-2025
Affiliated Hospital of Southwest Medical University
2025
Taipei Veterans General Hospital
2021
Sichuan Agricultural University
2020
Soochow University
2019-2020
Tianjin University of Traditional Chinese Medicine
2020
University of Arizona
2012-2019
Guangzhou Medical University
2017-2018
National Taiwan University
2017
Abstract Background Using a toxin-induced nonhuman primate model of acute liver failure (ALF), we previously reported that peripheral infusion human umbilical cord mesenchymal stem cells (hUC-MSCs) strongly suppresses the activation circulating monocytes and interleukin-6 (IL-6) production, thereby disrupting development cytokine storm improving prognosis monkeys. MSCs are considered to play therapeutic role under different stresses by adaptively producing specific factors, prompting us...
Tartary buckwheat (Fagopyrum tataricum) is rich in flavonols, which are thought to be highly beneficial for human health. However, little known about the regulatory mechanism of flavonol biosynthesis buckwheat. In this study, we identified and characterized a novel SG7 R2R3-MYB transcription factor buckwheat, FtMYB6. We showed that FtMYB6 located nucleus acts as transcriptional activator. The promoter strong spatiotemporal specificity was induced by light. expression significant correlation...
Overexpression of platelet-derived growth factor receptor β (PDGFR-β) has been associated with cancers and vascular fibrotic disorders. PDGFR-β become an attractive target for the treatment DNA G-quadruplexes formed in GC-rich nuclease hypersensitivity element human gene promoter have found to inhibit transcriptional activity. Here we determined major G-quadruplex promoter. Instead using four continuous runs three or more guanines, this adopts a novel folding broken G-strand form primarily...
Liver cancer and other malignant tumor cells rely on the glycolytic pathway to obtain energy (i.e. Warburg effect); however, underlying mechanism is unclear. Mitochondria are sites of oxidative phosphorylation adenosine triphosphate (ATP) production. The 13 constituent respiratory chain proteins encoded by mitochondrial genome (namely, mtDNA) play essential roles. We found that in human hepatocellular carcinoma (HCC) tissues, 11 out mtDNA-encoded genes exhibited decreased mRNA levels 5...
Abstract Objective: The senescence of nucleus pulposus (NP) cells induced by oxidative stress is one the important causes intervertebral disc degeneration (IDD). Herein, we investigated role and action mechanism silent information regulator 1 (SIRT1) in stress-induced rat NP cell. Methods: Premature was sublethal concentration hydrogen peroxide (H2O2) (100 μM). SIRT1 activated with SRT1720 (5 μM) to explore its effect on senescence. FoxO1 Akt were inhibited AS1842856 (0.2 MK-2206 μM),...
Background & AimsN6-methyladenosine (m6A), the most prevalent and dynamic posttranscriptional methylation modification of mammalian mRNA, is involved in various biological processes, but its role liver regeneration has not been characterized.MethodsWe first conducted transcriptome-wide m6A mRNA sequencing characterized expression pattern regenerating mouse liver. Next, we generated hepatocyte-specific Mettl3- or Mettl14-deficient mice investigated their regeneration. A series biochemical...
<title>Abstract</title> The global incidence of obesity and its metabolic sequelae, notably dysfunction-associated steatohepatitis (MASLD), has escalated to epidemic levels. We unveil a previously unknown moonlighting role for arginase 1 (Arg1) in facilitating hepatic lipogenesis. Mice lacking Arg1 exhibited diminished lipid accumulation both liver adipocytes, an effect mirrored genetically- or diet-induced models following inhibitor treatment. Mechanistically, competes with RSK2 Elk1...
The important MYC oncogene is deregulated in many cancer cells and comprises one of the most prominent G-quadruplex (G4) forming sequences its promoter regions, NHE III1 motif. Formation G4s suppresses transcription can be modulated by drug binding, establishing these DNA structures as promising targets therapy. motif fold into more than parallel G4s, including 1:2:1 1:6:1 loop length conformers, with conformer shown major species under physiological conditions solution. However, additional...
Intrahepatic cholangiocarcinoma (iCCA) is closely correlated with hepatic progenitor cell (HPC) expansion and liver fibrosis. Brahma-related gene 1 (Brg1), an enzymatic subunit of the switch/sucrose nonfermentable complex that critical in stem maintenance tumor promotion, prominently up-regulated both HPCs iCCA; however, its role this correlation remains undefined.A retrospective cohort study indicated high Brg1 expression suggests poor prognosis patients iCCA. In chronically injured livers...
Abstract Numb, a stem cell fate determinant, acts as tumor suppressor and is closely related to wide variety of malignancies. Intrahepatic cholangiocarcinoma (iCCA) originates from hepatic progenitors (HPCs); however, the role Numb in HPC malignant transformation iCCA development still unclear. A retrospective cohort study indicated that was frequently decreased tissues suggests poor prognosis patients. Consistently, chemically induced mouse model, downregulated cells compared normal...
Abstract The chromatin remodeling complex SWI/SNF regulates the accessibility of target genes to transcription factors and plays a critical role in tumorigenesis hepatocellular carcinoma (HCC). is assembled from approximately 15 subunits, most these subunits have distinct roles are often aberrantly expressed HCC. A comprehensive exploration expression clinical significance would be great value. In present study, we obtained gene profile each subunit corresponding information Cancer Genome...
The prevalence of food allergy has increased in recent years, and Korean pine vicilin is a potential allergen. We have previously reported the crystallization purified from raw nut. Here we report isolation mRNA crystal structure at 2.40 Å resolution. overall nut similar to structures other 7S seed storage proteins consists an N-terminal domain C-terminal domain. Each assumes cupin fold, they are symmetrically related about pseudodyad axis. Three molecules form doughnut-shaped trimer through...
Coconut (Cocos nucifera) is an important palm tree. fruit widely consumed. The most abundant storage protein in coconut cocosin (a likely food allergen), which belongs to the 11S globulin family. Cocosin was crystallized near a century ago, but its structure remains unknown. By optimizing crystallization conditions and cryoprotectant solutions, we were able obtain crystals that diffracted 1.85 Å. gene cloned from genomic DNA isolated dry tissue. sequence deduced predicted coding used guide...
Glutamine synthetase (GS) and arginase 1 (Arg1) are widely used pathological markers that discriminate hepatocellular carcinoma (HCC) from intrahepatic cholangiocarcinoma; however, their clinical significance in HCC remains unclear.We retrospectively analyzed 431 patients: 251 received hepatectomy alone, the other 180 sorafenib as adjuvant treatment after hepatectomy. Expression of GS Arg1 tumor specimens was evaluated using immunostaining. mRNA sequencing immunostaining to detect progenitor...
Rationale: Congenital biliary atresia (BA) is a destructive obliterative cholangiopathy of neonates that affects both intrahepatic and extrahepatic bile ducts. However, the cause BA largely unknown. Methods: We explored cell junctions polarity complexes in early biopsy livers by immunofluorescence staining western blot. Cdc42, as key junction regulator, was found dramatically decreased livers. Therefore, order to investigate role Cdc42 development, we constructed liver-specific tamoxifen...
Abstract Background Hepatocellular carcinoma (HCC), the most common primary liver cancer, prevails mainly in males and has long been attributed to androgens higher circumstantial levels of interleukin-6 (IL-6) produced by resident hepatic macrophages. Methods Constitutively hepatocyte-specific histone deacetylase 3 (HDAC3)-deficient ( HDAC3 LCKO ) mice constitutively knockout systemic IL-6 simultaneously ablated & −/− were used our study explore causes sex differences HCC. Additionally,...