A5100629045- ATP Synthase and ATPases Research
- Photosynthetic Processes and Mechanisms
- Neuroscience and Neuropharmacology Research
- Molecular Sensors and Ion Detection
- Mitochondrial Function and Pathology
- Advanced Electron Microscopy Techniques and Applications
- Vibrio bacteria research studies
- Crystal structures of chemical compounds
- Atherosclerosis and Cardiovascular Diseases
- Lanthanide and Transition Metal Complexes
- Lipid Membrane Structure and Behavior
- Radiomics and Machine Learning in Medical Imaging
- T-cell and Retrovirus Studies
- PARP inhibition in cancer therapy
- Cancer, Lipids, and Metabolism
- Pharmacy and Medical Practices
- Ion Transport and Channel Regulation
- Advanced battery technologies research
- Viral Infections and Immunology Research
- Ion channel regulation and function
- Adipokines, Inflammation, and Metabolic Diseases
- Antibiotic Use and Resistance
- Aquaculture disease management and microbiota
- Axial and Atropisomeric Chirality Synthesis
- Transgenic Plants and Applications
Second Affiliated Hospital of Zhejiang University
2024
Ningxia Medical University
2023
Ningxia Medical University General Hospital
2023
MRC Laboratory of Molecular Biology
2020-2022
Liaocheng University
2022
Institute of Biophysics
2017-2021
Chinese Academy of Sciences
2017-2021
University of Chinese Academy of Sciences
2017-2021
North China University of Science and Technology
2020
Czech Academy of Sciences, Institute of Biophysics
2019
Mammalian mitochondrial inner membrane fusion is mediated by optic atrophy 1 (OPA1). Under physiological conditions, OPA1 undergoes proteolytic processing to form a membrane-anchored long isoform (L-OPA1) and soluble short (S-OPA1). A combination of L-OPA1 S-OPA1 essential for efficient fusion; however, the relevant mechanism not well understood. In this study, we investigate cryo-electron microscopic structures S-OPA1-coated liposomes in nucleotide-free GTPγS-bound states. exhibits general...
Abstract AMPA-type glutamate receptors (AMPARs) mediate rapid signal transmission at excitatory synapses in the brain. Glutamate binding to receptor’s ligand-binding domains (LBDs) leads ion channel activation and desensitization. Gating kinetics shape synaptic are strongly modulated by transmembrane AMPAR regulatory proteins (TARPs) through currently incompletely resolved mechanisms. Here, electron cryo-microscopy structures of GluA1/2 TARP-γ8 complex, both open desensitized states (at 3.5...
Abstract Atherosclerosis (AS), a leading cause of cardio-cerebrovascular disease worldwide, is driven by the accumulation lipid contents and chronic inflammation. Traditional strategies primarily focus on reduction to control AS progression, leaving residual inflammatory risks for major adverse cardiovascular events (MACEs). While anti-inflammatory therapies targeting innate immunity have reduced MACEs, many patients continue face significant risks. Another key component in progression...
Surgical resection is the primary treatment for hepatocellular carcinoma (HCC). However, studies indicate that nearly 70% of patients experience HCC recurrence within five years following hepatectomy. The earlier recurrence, worse prognosis. Current on postoperative primarily rely pathology and patient clinical data, which are lagging. Hence, developing a new pre-operative prediction model crucial guiding individualized enhancing their prognosis.To identify key variables in imaging data...
Abstract The heme‐copper oxidase superfamily comprises cytochrome c and ubiquinol oxidases. These enzymes catalyze the transfer of electrons from different electron donors onto molecular oxygen. A B‐family hyperthermophilic bacterium Aquifex aeolicus was discovered previously to be able use both naphthoquinol as donors. Its mechanism well evolutionary significance are yet unknown. Here we solved its 3.4 Å resolution cryo‐microscopic structure a novel dimeric mediated by subunit I (CoxA2)...
ABSTRACT Recent revolution of cryo-electron microscopy has opened a new door to solve high-resolution structures macromolecule complexes without crystallization while how efficiently obtain homogenous complex sample is therefore becoming bottleneck. Here we report SmartBac, an easy and versatile system for constructing large-sized transfer plasmids used generate recombinant baculoviruses that express large multiprotein in insect cells. The SmartBac integrates the univector plasmid-fusion...
ABSTRACT Mammalian mitochondrial inner membrane fusion is mediated by OPA1(optic atrophy 1). Under physiological condition, OPA1 undergoes proteolytic processing to form a membrane-anchored long isoform (LOPA1) and soluble short (SOPA1). A combination of LOPA1 SOPA1 are required for fusion, however, the relevant mechanism not well understood. In this study, we investigate cryo-EM structures coated liposome tube at nucleotide-free GTPγS bound states. exhibits general structure dynamin family...
ABSTACT Cryo-electron tomography (cryo-ET) provides a promising technique to study high resolution structures of macromolecules in situ , opening new era structural biology. One major bottleneck this is prepare suitable cryo-lamellas cell/tissue samples. The emergence cryo-focused ion beam (cryo-FIB) milling good solution bottleneck. However, there are still large limitations using cryo-FIB cryo-lamella tissue specimen because the thickness increases difficulty freezing and milling. Here we...
Abstract AMPA glutamate receptors (AMPARs) mediate the majority of excitatory transmission in brain, and enable synaptic plasticity that underlies learning 1. A diverse array AMPAR signaling complexes are established by receptor auxiliary subunits, associating various combinations to modulate trafficking, gating strength 2. However, their mechanisms action poorly understood. Here, we determine cryo-electron microscopy structures heteromeric GluA1/2 assembled with both TARP-γ8 CNIH2,...
Abstract C 40 H 36 Cl 2 N O , orthorhombic, Pbca (no. 61), a = 10.6137(2) Å, b 20.1559(4) c 31.4991(5) V 6738.6(2) Å 3 Z 8, R gt ( F ) 0.0471, wR ref 0.1242, T 128 K.
Abstract The heme‐copper oxidase superfamily comprises cytochrome c and ubiquinol oxidases. These enzymes catalyze the transfer of electrons from different electron donors onto molecular oxygen. A B‐family hyperthermophilic bacterium Aquifex aeolicus was discovered previously to be able use both naphthoquinol as donors. Its mechanism well evolutionary significance are yet unknown. Here we solved its 3.4 Å resolution cryo‐microscopic structure a novel dimeric mediated by subunit I (CoxA2)...
Objective To evaluate the genotype polymorphism of V.parahaemolyticus using enterobacterial repetitive intergenic consensus (ERIC) -PCR and explore its application in distinguishing sporadic or outbreak cases V.parahaemolyticus. Methods One hundred eighty-seven samples isolated from patients,seafood,marine estuarine environments Ningbo city were typed with both anti-sera ERIC-PCR.The ERIC-PCR typing results analyzed cluster analysis method to variation ERIC sequences...
Objective To analyze the etiological characteristics of a Vibrio parahemolyticus caused foodborne-disease outbreak in Ningbo summer,2016.Methods The V.parahemolyticus strains isolated were serotyped and their susceptibilities to 10 antibiotics detected.Real time fluorescence quantitative PCR was used detect tdh,trh tlh genes.The cluster analysis 8 performed by using pulsed-field gel electrophoresis (PFGE).Results A total from stool samples diarrhea patients.Serological O K antigens indicated...
Abstract C 45 H 48 N 2 O 5 , monoclinic, P 1 / c (no. 14), a = 21.3971(8) Å, b 15.2249(6) 11.2610(5) β 92.125(4)°, V 3666.0(3) Å 3 Z 4, R gt ( F ) 0.0519, wR ref 0.1308, T 128.15 K.
Abstract AMPA glutamate receptors (AMPARs) mediate the majority of excitatory transmission in brain, and enable synaptic plasticity that underlies learning 1 . A diverse array AMPAR signaling complexes are established by receptor auxiliary subunits, associating various combinations to modulate trafficking, gating strength 2 However, their mechanisms action poorly understood. Here, we determine cryo-electron microscopy structures heteromeric GluA1/2 assembled with both TARP-γ8 CNIH2,...
The development of bifunctional electrocatalysts to reduce the polarization loss is essential enhance performance Zn-air batteries. combination metal-organic frameworks (MOFs) with metal or metallic compounds provides a way integrate their merits and effectively improve electrocatalytic performances. However, previous synthesis MOFs/metal oxides usually involves high-temperature calcination, which makes it difficult control morphologies. In this work, through ligand-insufficient high...