A5100456103- Mitochondrial Function and Pathology
- ATP Synthase and ATPases Research
- Photosynthetic Processes and Mechanisms
- Cellular transport and secretion
- Advanced Electron Microscopy Techniques and Applications
- Metabolism and Genetic Disorders
- Lipid Membrane Structure and Behavior
- Hemoglobin structure and function
- Force Microscopy Techniques and Applications
- Polymer Nanocomposites and Properties
- Cellular Mechanics and Interactions
- Endoplasmic Reticulum Stress and Disease
- Metalloenzymes and iron-sulfur proteins
- Fiber-reinforced polymer composites
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Enzyme Structure and Function
- Mycorrhizal Fungi and Plant Interactions
- Hemoglobinopathies and Related Disorders
- Cancer, Hypoxia, and Metabolism
- Genetic Neurodegenerative Diseases
- Calcium signaling and nucleotide metabolism
- RNA modifications and cancer
- Electron and X-Ray Spectroscopy Techniques
- Image Processing Techniques and Applications
Institute of Biophysics
2013-2025
Chinese Academy of Sciences
2016-2025
University of Chinese Academy of Sciences
2021-2025
Institute of Botany
2025
Institute of Information Engineering
2025
Jiangnan University
2024
Czech Academy of Sciences, Institute of Biophysics
2019-2024
Shanghai Jiao Tong University
2024
Shanghai Institute of Ceramics
2023
ShanghaiTech University
2023
Ryanodine receptors (RyRs) are a class of giant ion channels with molecular mass over 2.2 mega-Daltons. These mediate calcium signaling in variety cells. Since more than 80% the RyR protein is folded into cytoplasmic assembly and remaining residues form transmembrane domain, it has been hypothesized that activation regulation occur through an as yet uncharacterized long-range allosteric mechanism. Here we report characterization Ca2+-activated open-state RyR1 structure by cryo-electron...
Abstract Lipoprotein biogenesis is essential for bacterial survival. Phosphatidylglycerol:prolipoprotein diacylglyceryl transferase (Lgt) an integral membrane enzyme that catalyses the first reaction of three-step post-translational lipid modification. Deletion lgt gene lethal to most Gram-negative bacteria. Here we present crystal structures Escherichia coli Lgt in complex with phosphatidylglycerol and inhibitor palmitic acid at 1.9 1.6 Å resolution, respectively. The reveal presence two...
Mammalian mitochondrial inner membrane fusion is mediated by optic atrophy 1 (OPA1). Under physiological conditions, OPA1 undergoes proteolytic processing to form a membrane-anchored long isoform (L-OPA1) and soluble short (S-OPA1). A combination of L-OPA1 S-OPA1 essential for efficient fusion; however, the relevant mechanism not well understood. In this study, we investigate cryo-electron microscopic structures S-OPA1-coated liposomes in nucleotide-free GTPγS-bound states. exhibits general...
Papiliomyces (Hypocreales, Sordariomycetes) was introduced to accommodate two species: Papiliomycesliangshanensis and Papiliomycesshibinensis. Later, renamed Ophiocordycepsliangshanensis. However, the molecular data (Nepalese) used establish genus different from Ophiocordycepsliangshanensis (China) strains.This paper describes a new species, Papiliomyceslongiclavatus, found in Yangchang District, Guiyang City, Guizhou Province, China. It is proposed, based on morphology multilocus phylogeny...
ADP-Ribosylation Factor (ARF) small GTPases have been found to act in vesicle fission through a direct ability tubulate membrane. We pursued cryoelectron microscopy (EM) reveal at 3.9 Å resolution how ARF6 assembles into protein lattice on tubulated Molecular dynamics simulation studies confirm and extend the cryo-EM findings. The exhibits features that are distinct from those formed by other membrane-bending proteins. identify contacts critical for assembly membrane insertion results...
Summary Most rice varieties are able to grow in red high‐Fe soil, but the underlying mechanism remains elusive. Through forward genetic screening, we identified a soil‐sensitive ‐ 1 ( rss1 ) mutant that exhibited severely retarded growth when grown soil showed no evident phenotype cinnamon soil. Under soil/high‐Fe conditions, increased Fe decreased copper (Cu) concentrations both roots and shoots, was partially rescued by Cu supplement. RSS1 encodes an OsSPL9 transcription factor is...
Fusion controls mitochondrial morphology and is important for normal function, including roles in respiration, development, apoptosis. Key components of the fusion machinery have been identified, allowing an initial dissection its molecular mechanism. Outer inner membrane events are coordinately coupled but mechanistically distinct. Mitofusins GTPases that likely mediate outer fusion. The dynamin-related protein OPA1/Mgm1p required maintenance cristae structure. We highlight recent findings...
Mitochondria transport and utilize iron for the synthesis of haem Fe-S clusters. Although many proteins are known to be involved in these processes, additional likely participate. To test this hypothesis, present study we used a genetic screen looking yeast mutants that synthetically lethal with mitochondrial carriers Mrs3 Mrs4. Several genes were identified, including an isolate mutated Yfh1, frataxin homologue. All such triple complemented by increased expression Rim2, another carrier...
Mitochondria are highly-dynamic organelles, but it is challenging to monitor quantitatively their dynamics in a living cell. Here we developed novel approach determine the global occurrence of mitochondrial fission and fusion events human epithelial cells (Hela) mouse embryonic fibroblast (MEF). Distinct patterns sequential including followed by (Fu-Fi), so-called "kiss run" model previously described, (Fi-Fu), (Fu-Fu), (Fi-Fi) were observed concurrently. The paired appeared high frequencies...
Mammalian oocyte chromosomes undergo 2 meiotic divisions to generate haploid gametes. The frequency of chromosome segregation errors during meiosis I increase with age. However, little attention has been paid the question how aging affects sister chromatid II. More importantly, aneuploid metaphase II (MII) oocytes from aged mice evade spindle assembly checkpoint (SAC) mechanism complete later form embryos remains unknown. Here, we report that MII naturally exhibited substantial in...
Actins are among the most abundant and conserved proteins in eukaryotic cells, where they form filamentous structures that perform vital roles key cellular processes. Although large amounts of data on biochemical activities, dynamic behaviors, important functions plant actin filaments have accumulated, their structural basis remains elusive. Here, we report a 3.9 Å structure filament from
Significance Coat proteins play a central role in the intracellular transport pathways by coupling two main functions: bending membrane to generate carriers and binding cargoes for their sorting into these carriers. Studies thus far have mostly solved structure of coat solution, but functional form requires assembly on protein complexes. Here, we pursued cryo-EM reveal molecular detail how SNX1 assembles deform membrane. When compared previously retromer-SNX complex, our elucidation also...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe electronic structures of active sites in non-heme iron enzymesEdward I. Solomon and Yan ZhangCite this: Acc. Chem. Res. 1992, 25, 8, 343–352Publication Date (Print):August 1, 1992Publication History Published online1 May 2002Published inissue 1 August 1992https://pubs.acs.org/doi/10.1021/ar00020a004https://doi.org/10.1021/ar00020a004research-articleACS PublicationsRequest reuse permissionsArticle Views384Altmetric-Citations35LEARN ABOUT THESE...
Abstract Background Glioma is a common primary central nervous system tumour, and therapeutic drugs that can effectively improve the survival rate of patients in clinic are lacking. Bufalin effective treating various tumours, but mechanism by which it promotes apoptosis glioma cells unclear. The aim this study was to investigate drug targets bufalin clarify apoptotic mechanism. Methods Cell viability proliferation were evaluated CCK-8 colony formation assays. Then, cell cycle apoptosis,...
The archael small heat-shock protein (sHSP), MjHSP16.5, forms a 24-subunit oligomer with octahedral symmetry. Here, we demonstrate that the IXI motif present in C-terminal domain is necessary for oligomerization of MjHSP16.5. Removal increased vitro chaperone activity citrate synthase as client protein. Less predictable were effects R107G substitution MjHSP16.5 because differences metazoan and non-metazoan sHSPs. We crystal structure compare this an improved (2.5 Å) wild-type (WT) Although...
Abstract Volume electron microscopy (vEM) has become a rapidly developing technique for studying the 3D architecture of biological specimens, such as cells, tissues, and organs at nanometer resolution; this involves collecting series micrographs axial sequential sections reconstructing volume, providing useful information on cellular ultrastructural spectrum. This currently suffers from anisotropic resolution between lateral (x, y) (z) directions loss/damage sections. Here, we develop new...