The epigenetic status of a donor nucleus has an important effect on the developmental potential embryos produced by somatic cell nuclear transfer (SCNT). In this study, we transferred cultured rabbit cumulus cells (RCC) and fetal fibroblasts (RFF) from genetically marked rabbits (Alicia/Basilea) into metaphase II oocytes analyzed levels histone H3-lysine 9-lysine 14 acetylation (acH3K9/14) in cloned embryos. We also assessed correlation between their potential. To test whether alteration...

In mammals the parental genomes are epigenetically reprogrammed after fertilization. This reprogramming includes a rapid demethylation of paternal (sperm-derived) chromosomes prior to DNA replication in zygotes. Such active zygote has been documented for several mammalian species, including mouse, rat, pig, human and cow, but questioned occur rabbit.When comparing immunohistochemical patterns antibodies against 5-methyl-cytosine, H3K4me3 H3K9me2 modifications we observe similar pronuclear...

Introduction Lyme arthritis (LA) is a chronic inflammatory joint disease caused by Borrelia burgdorferi infection, with approximately 10-20% of patients developing antibiotic resistance. Macrophages in the synovial microenvironment play crucial role progression, but their precise regulatory mechanisms remain unclear. Methods We analyzed scRNA-seq data (GSE233850) from LA mouse tissue and integrated transcriptomic models GSE125503 dataset. Cell clustering, differential gene analysis, GO KEGG...

Neurovascular cells have wide-ranging implications on skeletal muscle biology regulating myogenesis, maturation, and regeneration. Although several in vitro studies investigated how motor neurons endothelial interact with myocytes independently, there is limited knowledge about the combined effect of neural vascular maturation development. Here, we report a triculture system comprising human-induced pluripotent stem cell (iPSC)-derived myocytes, human iPSC-derived neurons, primary maintained...

Beckwith-Wiedemann Syndrome (BWS) is an epigenetic overgrowth syndrome caused by methylation changes in the human 11p15 chromosomal locus. Patients with BWS may exhibit hepatomegaly, as well increased risk of hepatoblastoma. To understand impact these liver, we performed a multiomic study [single nucleus RNA-sequencing (snRNA-seq) + single assay for transposable-accessible chromatin-sequencing (snATAC-seq)] both BWS-liver and nonBWS-liver tumor-adjacent tissue. Our approach uncovers...

Cell-based therapeutic strategies afford major potential advantages in the repair of injured tendons. Generation induced pluripotent stem cells (iPSCs) expands cell sources for "regenerative" therapy. However, its application tendon is still limited and effects remain unclear. In this study, equine tenocyte-derived iPSCs (teno-iPSCs) were generated by expressing four Yamanaka factors. Compared to parental tenocytes bone marrow derived mesenchymal (BMSCs), transcriptional activities...

Histone acetylation regulates higher-order chromatin structure and function is critical for the control of gene expression. deacetylase inhibitors (HDACi) are currently un¬der investigation as novel cancer therapeutic drugs. Here, we show that female germ cells extremely suscep¬tible to changes induced by HDACi. Our results indicate exposure trichostatin A (TSA) at nanomolar levels interferes with major remodeling events in mammalian oocyte leading chromosome instability. High resolution...

Chronic environmental exposure to metal toxicants such as chromium and arsenic is closely related the development of several types common cancers. Genetic epigenetic studies in past decade reveal that post-translational modifications histones play a role carcinogenesis. However, exact molecular mechanisms carcinogenesis remain be elucidated. In this study we found As2O3, an toxicant, upregulated overall many cellular proteins by SUMO2/3. Sumoylated from arsenic-treated cells constitutively...

BubR1 is an important component of the spindle assembly checkpoint, and deregulated functions frequently result in chromosomal instability malignant transformation. We recently demonstrated that was modified by sumoylation, lysine 250 (K250) as crucial site for this modification. sumoylation neither required its activation nor binding to kinetochores. However, ectopically expressed sumoylation-deficient mutants were retained on kintochores even after apparent chromosome congression. The...

The tendon is highly prone to injury, overuse, or age-related degeneration in both humans and horses. Natural healing of injured poor, cell-based therapeutic treatment still a significant clinical challenge. In this study, we extensively investigated the expression tenogenic genes equine bone marrow mesenchymal stem cells (BMSCs) tenocyte-derived induced pluripotent (teno-iPSCs) stimulated by growth factors (TGF-β3 BMP12) combined with ectopic transcription factor MKX cyclic uniaxial...

Disruption of cell cycle checkpoints and interference with the normal progression frequently result in death or malignant transformation. Hexavalent chromium [Cr(VI)] is a well-known carcinogen that has been implicated occurrence many types human malignancies, including lung cancer. However, exact mechanism by which Cr(VI) causes transformation remains unknown. We have demonstrated chronic exposure to non-cytotoxic concentration induced variety chromosomal abnormalities, premature sister...

SALL4B plays a critical role in maintaining the pluripotency of embryonic stem cells and hematopoietic cells. primarily functions as transcription factor, and, thus, its nuclear localization is paramount to biological activities. To understand structural basis by which was transported retained nucleus, we made series constructs with deletions or point mutations. We found that K64R mutation resulted random distribution within cell. An analysis neighboring amino acid sequences revealed...

Mps1 is a dual specificity protein kinase that regulates the spindle assembly checkpoint and mediates proper microtubule attachment to chromosomes during mitosis. However, molecular mechanism controls level its activity cell cycle remains unclear. Given sumoylation plays an important role in mitotic progression, we investigated whether was SUMO-modified affects Our results showed sumoylated both asynchronized populations. modified by SUMO-1 SUMO-2. further studies revealed lysine residues...

Kif18A, the kinesin-8 motor protein, plays an essential role in regulating alignment of bi-oriented chromosomes at midzone during mitosis. Kinesin proteins, including are often deregulated many types cancers and thought to play a critical cancer progression. However, little is known about post-translational modifications Kif18A their effects on its biological activity.Kif18A was identified be SUMO2 acceptor by using Ni-IDA resin precipitate proteins from cells stably expressing His6-SUMO2....

IK is a nuclear protein containing unique domain named RED due to the presence of repetitive arginine (R), aspartic (E), and glutamic acid (D) sequence. To date, function this remains largely unknown despite couple previous studies in literature. Here we report that depletion via RNA interference results mitotic arrest. We also demonstrate undergoes dynamic translocation during interphase mitosis. In particular, primarily present some cells as foci/bodies which do not co-localize with...

VOLUME 287 (2012) PAGES 38600–38608 PAGE 38605: Fig. 5B did not conform with the JBC policy that figures assembled from separate images should indicate borders between images. This figure has been corrected. correction does change results or conclusions of this work.

VOLUME 287 (2012) PAGES 4875–4882 PAGE 4877: Fig. 2A did not conform with the JBC policy that figures assembled from separate images should indicate borders between images. This figure has been corrected. correction does change results or conclusions of this work. 4878: 3C 4879: Incorrect immunoblots were used in vehicle and taxol plus caffeine (T+C) lanes 5C. error 4881: 7B

In mammals, upon the penetration of sperm into oocyte, paternal genome undergoes dramatic epigenetic changes. Protamin packaging DNA is replaced by histones that acquire specific modifications. mouse zygotes, gets rapidly demethylated an active mechanism. bovine zygotes methylation from erased only partially, and in rabbit it persists at initial level. To understand whether these reprogramming differences are also reflected histone modifications, we examined dynamic changes H3 positions K4...

Cloned rabbits have been obtained by somatic cell nuclear transfer (SCNT) only with fresh, non-cultured cumulus cells (Chesne et al. 2002 Nat. Biotechnol. 20, 366–369). For the purpose of generating transgenic animals SCNT, donor must be cultured and modified prior to use as donors. The objective this study was optimize SCNT procedure using or fibroblast cells. MII oocytes were harvested from superovulated Zika rabbits, maternal chromosomes removed demecolcine-assisted enucleation (Yin Biol....