A5065587628- Cellular Mechanics and Interactions
- Bone Tissue Engineering Materials
- Nanoparticle-Based Drug Delivery
- Graphene and Nanomaterials Applications
- Cell Adhesion Molecules Research
- SARS-CoV-2 detection and testing
- Iron oxide chemistry and applications
- Ubiquitin and proteasome pathways
- 3D Printing in Biomedical Research
- Biosensors and Analytical Detection
- Curcumin's Biomedical Applications
- Hair Growth and Disorders
- Hedgehog Signaling Pathway Studies
- Neuroscience and Neural Engineering
- Geotechnical and Geomechanical Engineering
- Cancer-related molecular mechanisms research
- Cardiac Fibrosis and Remodeling
- Polymer Surface Interaction Studies
- Characterization and Applications of Magnetic Nanoparticles
- Aluminum toxicity and tolerance in plants and animals
- Environmental remediation with nanomaterials
- Mesenchymal stem cell research
- Cancer and Skin Lesions
- Dendrimers and Hyperbranched Polymers
- Advanced Memory and Neural Computing
Korea University
2020-2024
Southwest Jiaotong University
2016-2022
Peking University
2011
Peking University First Hospital
2011
Hospital for Sick Children
2003
SickKids Foundation
2003
University of Michigan
2003
University of Toronto
2003
Sonic hedgehog (Shh) signaling plays a critical role in hair follicle development and skin cancer, but how it controls these processes remains unclear. Of the three Gli transcription factors involved transducing Shh signals vertebrates, we demonstrate here that Gli2 is key mediator of responses skin. Similar to Shh(-/-) mice, Gli2(-/-) mutants exhibit an arrest with reduced cell proliferation Shh-responsive gene expression, grossly normal epidermal differentiation. By transgenic rescue...
Circulating tumor DNA (ctDNA) detection has been acknowledged as a promising liquid biopsy approach for cancer diagnosis, with various ctDNA assays used early and treatment monitoring. Dispersible magnetic nanoparticle-based electrochemical methods have proposed candidates based on the performance features of platform material. This study proposes nanoparticle surface-localized genetic amplification by integrating Fe3O4–Au core–shell nanoparticles into polymerase chain reactions (PCR). These...
Abstract Design of materials with remote switchability the movement decorated nanostructures presenting cell‐adhesive Arg‐Gly‐Asp ligand can decipher dynamic cell‐material interactions in nanostructures. In this study, decoration ligand‐bearing gold nanoparticles (ligand‐AuNPs) on magnetic nanoparticle (MNP) varying ligand‐AuNP densities is demonstrated, which are flexibly coupled to substrate various MNP maintain constant macroscopic density. Magnetic switching upward (“Upper Mag”) or...
Abstract Cellular adhesion is regulated by the binding of 10 nm sized integrin to Arg‐Gly‐Asp (RGD) ligands present in extracellular matrix proteins. In this study, seed‐mediated growth gold nanoparticles (AuNPs) on surface iron oxide (Fe 3 O 4 ) nanotemplates employed tune diameter and interdistance RGD‐bearing AuNPs at receptor‐level. The Fe decorated with arranged various RGD diameters interdistances receptor‐levels are flexibly fastened a substrate. Similar fully connected RGDs,...
Cell adhesion occurs when integrin recognizes and binds to Arg-Gly-Asp (RGD) ligands present in fibronectin. In this work, submolecular ligand size spacing are tuned via template-mediated situ growth of nanoparticles for dynamic macrophage modulation. To tune liganded gold nanoparticle (GNP) from 3 20 nm, localized assemblies GNP arrays on nanomagnetite templates engineered. nm-spaced stimulate the binding integrin, which mediates macrophage-adhesion-assisted pro-regenerative polarization as...
Of various molecular diagnostic assays, the real-time reverse transcription polymerase chain reaction is considered gold standard for infection diagnosis, despite critical drawbacks that limit rapid detection and accessibility. To confront these issues, several nanoparticle-based methods have been developed to a great extent, but still possess challenges. In this study, novel nucleic acid amplification method termed surface localized (nSLAM) paired with electrochemical (ECD) develop...
Abstract The native extracellular matrix (ECM) can exhibit heterogeneous nano‐sequences periodically displaying ligands to regulate complex cell–material interactions in vivo. Herein, an ECM‐emulating barcoding system, including ligand‐bearing Au and ligand‐free Fe nano‐segments, is developed independently present tunable frequency sequences nano‐segments of cell‐adhesive RGD ligand. Specifically, similar exposed surface areas total are designed. segments used for substrate coupling...
Abstract Native extracellular matrix (ECM) exhibits dynamic change in the ligand position. Herein, ECM‐emulating control and real‐time monitoring of stem cell differentiation are demonstrated by nanoassembly. The density gold nanoassembly presenting cell‐adhesive Arg‐Gly‐Asp (RGD) on Fe 3 O 4 (magnetite) nanoparticle nanostructures flexibly grafted to material is changed while keeping macroscale invariant. can be magnetically attracted mediate rising falling movements via linker stretching...
In the crystallisation of nanomaterials, an assembly-based mechanism termed 'oriented attachment' (OA) has recently been recognised as alternative crystal growth that cannot be explained by classical theory. However, attachment alignment during OA is not currently tuneable because its poorly understood. Here, we identify crystallographic disorder-order transitions in magnetite (Fe3O4) mesocrystals depending on types organic surface ligands building blocks, which produce different grain...
Cell Adhesion In article number 2110340, Ramasamy Paulmurugan, Young Keun Kim, Heemin Kang, and co-workers report template-mediated in situ tuning of nanoligand size spacing. They demonstrate that submolecular ligand spacing magnetic anchoring facilitate adhesion-structure assembly-mediated pro-healing polarization host macrophages. Increasing the significantly suppresses macrophage adhesion whereas increasing both slightly inhibits it.
Rotavirus is a leading cause of death in young children worldwide. Although vaccines are currently available and reduce the disease burden, most low-income countries rely on diagnosis by point-of-care testing owing to its rapidity, simplicity, affordability; however, sensitivity remains problem. Magnetically assisted antigen enrichment improves with increased fluorescence intensity. However, magnetic nanoparticles (NPs) strongly absorb ultraviolet light, limiting combination NPs quantum dots...
Micro/nanotopographies (MNTs) have been reported to enhance the osseointegration of biomaterials and modulate cell functions, but underlying mechanisms are incompletely understood. We hypothesized that transient receptor potential vanilloid 4 (TRPV4) may mediate topographically induced osteoblastic differentiation bone marrow stem cells (BMSCs) by regulating NFATc1 Wnt/β-catenin signaling. To test this hypothesis, murine BMSCs were cultured on polished titanium (Ti) discs (PT) Ti carrying...
Co-doping of multiple ions can effectively adjust the biological properties hydroxyapatite (HA) for various biomedical applications. In this study, we prepared Sr 2+ and Cu double-doped hollow HA characterized them by SEM, EDS, XRD, FTIR, other methods. We found that were uniformly distributed in carbonic acid microspheres. As proportion metal elements increases, microspherical appearance crystallinity also change. addition, porous titanium scaffolds through 3D printing technology...
Organic pollutants in wastewater pose serious threats to human health and the environment. Nevertheless, potent strategies are being devised tackle this critical issue. Specifically, advanced oxidation processes driven by solar energy photocatalysts show promise for degradation of organic pollutants. In particular, porous nanomaterials effective as because their high surface area, light‐harvesting properties, ability generate reactive oxygen species. To further expedite development feasible...
Multiyolk–Shell Nanocoils Article number 2312214 by Young Keun Kim and co-workers presents the synthesis strategy of eight distinct nanocoils featuring varied shapes compositions. These are derived from a bimetallic alloy nanocoil template through meticulous control electrochemical conditions. The resultant multiyolk-shell (mYSNC), synthesized within an electrochemically programmed aqueous solution, manifests distinctive unprecedented structural configuration. mYSNCs demonstrate exceptional...
Integrin Clustering In article number 2200828, Young Keun Kim, Heemin Kang, and co-workers report the dynamic tuning of receptor-level ligand proximity. Closely spaced ligands coated onto subordinate nanostructures distributed on a nano-template stimulate integrin binding clustering. This activates spreading, mechanotransduction, differentiation stem cells that occur in an active pro-regenerative microenvironment.
Abstract CDK5 belongs to the cycling dependent kinase family, which is multifunctional and plays an important role in neural differentiation. However, of osteoblastic differentiation remains unclear. The present study investigated functions molecular mechanism It was found that, addition inhibitor Seliciclib promoted expression Runx2 , ALP OCN OPN MSCs mineralization MC-3T3E1 cells. enhanced development F-actin, nuclear localization β-catenin YAP, as well RMRP RNA. When F-actin suppressed by...
In article number 2004300, Young Keun Kim, Heemin Kang, and co-workers report a magnetic nano-barcoding system that enables unique tunability of ligand frequency nano-sequences to effectively regulate stem cell adhesion with no systemic toxicity in vivo. Low-frequency terminally sequenced nano-ligands facilitate focal adhesion, mechanotransduction, differentiation cells.
In article number 2008698, Young Keun Kim, Heemin Kang, and co-workers report magnetic switching of the movement decorated ligand-presenting nanostructures that regulates adhesion-mediated polarization macrophages in vivo. Remote downward facilitates macrophage adhesion-regulated M2 polarization, which is conversely suppressed by their upward movement.
CDK5 belongs to the cyclin-dependent kinase family. is multifunctional and plays an important role in neural differentiation. However, of osteoblastic differentiation remains unclear. The present study investigated functions molecular mechanism It was found that, inhibition promoted expression Runx2, ALP, OCN OPN MSCs mineralization MC-3T3E1 cells MSCs. enhanced development F-actin, nuclear localization β-catenin YAP, as well RMRP RNA. When F-actin suppressed by Blebbistatin, YAP β-catenin,...