A5036512836- Dermatologic Treatments and Research
- Wound Healing and Treatments
- Hair Growth and Disorders
- Autoimmune Bullous Skin Diseases
- Hemostasis and retained surgical items
- Skin and Cellular Biology Research
- Dermatological and Skeletal Disorders
- Dermatology and Skin Diseases
- S100 Proteins and Annexins
- Helicobacter pylori-related gastroenterology studies
- Tissue Engineering and Regenerative Medicine
- Periodontal Regeneration and Treatments
- Pluripotent Stem Cells Research
- Cell Adhesion Molecules Research
- Psoriasis: Treatment and Pathogenesis
- Congenital limb and hand anomalies
- Connective Tissue Growth Factor Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Pressure Ulcer Prevention and Management
- Microscopic Colitis
- Saffron Plant Research Studies
- Galectins and Cancer Biology
- Genomics and Chromatin Dynamics
- Abdominal vascular conditions and treatments
- Sports Performance and Training
National Cheng Kung University Hospital
2019-2025
National Cheng Kung University
2018-2025
Northwestern University
2024-2025
Chang Hua Hospital
2011
Chang Gung Memorial Hospital
2008
Chang Gung University
2008
Abstract Background Pathologic scars, including keloids and hypertrophic represent a common form of exaggerated cutaneous scarring that is difficult to prevent or treat effectively. Additionally, the pathobiology pathologic scars remains poorly understood. We aim at investigating impact TEM1 (also known as endosialin CD248), which glycosylated type I transmembrane protein, on development scars. Methods To investigate expression TEM1, we utilized immunofluorescence staining, Western blotting,...
Oncogenic KRAS mutations are present in approximately 90% of pancreatic ductal adenocarcinoma (PDAC). However, Kras mutation alone is insufficient to transform precancerous cells into metastatic PDAC. This study investigates how KRAS-mutated epithelial acquire the capacity escape senescence or even immune clearance, thereby progressing advanced Single-cell RNA sequencing and analysis primary PDAC tumors were conducted. Genetically engineered pancreas-specific Kras-mutated, dual specificity...
Disseminated superficial actinic porokeratosis (DSAP) is characterized by numerous erythematous or brown papules plaques predominantly occurring in sun-exposed areas of the skin. Some DSAP patients experience moderate to severe itching that often proves resistant antihistamine treatment. The present case highlights potential guselkumab as a novel therapeutic approach for with intractable pruritus. successful treatment and subsequent RNA sequencing analysis suggest possible link between...
Hidradenitis suppurativa (HS) significantly diminishes the quality of life for patients. Delayed diagnosis represents a significant challenge in effectively managing HS.
Dominant dystrophic epidermolysis bullosa (DDEB) is characterized by trauma-induced blisters and, in some individuals, intense pruritus. Precisely what causes itch DDEB and optimal ways to reduce it have not been fully determined.
Previous studies have shown how adipocytes can modulate the activity of hair follicle stem cells. However, role in pathogenesis androgenetic alopecia (AGA) remains unknown. We aimed to determine signaling pathways related adipose tissue changes human scalp with AGA through RNA-seq analysis. RNA was isolated from tissues derived bald (frontal) and normal (occipital) scalps male patients ( n = 4). The pooled extracts these samples were subjected sequencing, followed by differential gene...
Keloids are a fibrotic skin disorder caused by abnormal wound healing and featuring the activation expansion of fibroblasts beyond original margin. Signal transducer activator transcription 3 (STAT3) has been found to mediate biological functions keloid (KFs). Therefore, we aimed demonstrate whether ASC-J9, an inhibitor STAT3 phosphorylation, can suppress KFs. Western blotting results showed that ASC-J9 inhibited levels COL1A1 FN1 proteins, which were upregulated in KFs, decreasing...
ABSTRACT Thrombomodulin (TM) is a transmembrane glycoprotein that contains five functional domains. Soluble TM (sTM), comprising extracellular domains TMD1 (lectin-like), TMD2 (epidermal growth factor [EGF]-like repeat containing), and TMD3 (serine-threonine rich), can be shed from cells by the intramembrane protease rhomboid-like-2 (RHBDL2). expressed osteoblasts, yet its role there has not been determined. Herein we aimed to investigate properties of in osteoblast function bone repair...
Generalized pustular psoriasis (GPP) is a rare but severe form of psoriasis. An early onset the diseases correlated with mutations among IL36RN, CARD14, AP1S3, MPO and SERPINA3 genes. Systemic biological agents including anti-TNF-α, anti-IL-17, anti-IL-12/IL-23, anti-IL1R, anti-IL1β anti-IL-36R act as novel treatment methods for GPP. Herein we report female infant clinically diagnosed GPP since she was 10-month-old. Results whole-exome sequencing (WES) Sanger revealed reported heterozygous...
Abstract Autosomal recessive congenital ichthyosis (ARCI) is a genetically heterogeneous disorder with aberrant skin scaling and increased transepidermal water loss (TEWL). Current treatments for ARCI are limited suboptimal. We present the case of 27-year-old man resulting from homozygous missense variant in TGM1. RNA-sequencing lesional revealed Janus kinase–signal transducer activator transcription signalling, providing rationale innovative treatment kinase inhibitor. prescribed oral...
Abstract Tumor endothelial marker 1 (TEM1) is a transmembrane glycoprotein that appears on mesenchymal lineage-derived cells during embryogenesis, but its expression greatly reduces after birth. Re-upregulation of TEM1 found in tumor angiogenesis, organ fibrosis and wound healing indicating potential role tissue remodeling repair. The level function adult heart are unknown. In explanted hearts from failure (HF) patients received cardiac transplantation, immunofluorescence staining showed was...
Dermatofibromas (DFs) are common, benign fibrous skin tumors that can occur at any site. In most cases, DFs solitary and sporadic, but a few multiple familial, the mechanisms leading to these lesions currently unclear. Using exome sequencing, we have identified heterozygous variant in pedigree with autosomal dominant familial DF within RND3 (c.692C>T,p.T231M) encodes for small GTPase RhoE, regulator of actin cytoskeleton. Expression T231M-RhoE or RhoE depletion using CRISPR human dermal...
Human adipose-derived stem cells (ASCs) have shown immense potential for regenerative medicine. Our previous work demonstrated that chitosan nano-deposited surfaces induce spheroid formation and differentiation of ASCs treating sciatic nerve injuries. However, the underlying cell fate mechanisms ASC-derived spheroids remain unknown. Here, we investigate epigenetic regulation signaling coordination these therapeutic spheroids. During formation, observed significant increases in histone 3...
Rubinstein-Taybi Syndrome (RSTS) is a rare congenital disease with distinctive facial features, broadening of the thumbs and halluces, developmental delay. RSTS caused by de novo genetic alterations in CREBBP homologous EP300 genes. In this study, we established diagnostic protocol integrating multiplex ligation-dependent probe amplification (MLPA) whole-exome sequencing (WES). Five patients clinically diagnosed were enrolled for testing. Germline DNA was extracted from peripheral blood...
Deleterious hyper-inflammation resulting from macrophage activation may aggravate sepsis and lead to lethality. Tumor endothelial marker 1 (TEM1), a type I transmembrane glycoprotein containing six functional domains, has been implicated in cancer chronic sterile inflammatory disorders. However, the role of TEM1 acute remains be determined. Herein we explored significance lectin-like domain (TEM1D1) monocyte/macrophage using TEM1D1-deleted (TEM1LeD/LeD) transgenic mice recombinant TEM1D1...
Capsular contracture is the most common reason for having a secondary breast implant operation. The failure of implanted device and discomfort are related to foreign body response, which involves pathologic encapsulation. An up-regulated expression CD248 was previously demonstrated modulate inflammation fibrosis. authors hypothesized that contributes reaction during silicone-stimulated capsule formation.
Tumor endothelial marker 1 (TEM1), an activated mesenchymal cell marker, is implicated in tissue remodeling and repair.Herein, we investigated the role therapeutic implications of TEM1 abdominal aortic aneurysm (AAA), a potentially life-threatening disease characterized by vascular inflammation matrix turnover.Characterization human AAA revealed increased expression derived mainly from medial smooth muscle cells (VSMCs) adventitial fibroblasts.Bioinformatics analysis demonstrated association...
In response to corneal injury, an activation of epithelial stem cells and their direct progeny the early transit amplifying (eTA) rapidly proliferate is critical for proper re-epithelialization. Thus, it important understand how such stem/eTA cell regulated. Angiotensin-converting enzyme 2 (ACE2) predominantly expressed in stem/eTA-enriched limbal epithelium but its role was unclear. Single RNA sequencing (scRNA-seq) suggested that Ace2 involved proliferation cells. reduced following injury....