Other SectionsABSTRACTINTRODUCTIONRESULTSDISCUSSIONMATERIALS AND METHODSACKNOWLEDGEMENTSCONFLICTS OF INTERESTSupplementary InformationFIGURESREFERENCES

Background: Growing evidence has demonstrated the important roles of gut microbiota and short chain fatty acids, especially acetate, propionate butyrate, in development obesity metabolic diseases.To date, effects butyrate on human adiposity glucose metabolism remain controversial.This study aimed to explore associations systemically with homeostasis patients type 2 diabetes (T2D) obesity.Methods: A total 12 T2D 8 age-and sex-matched healthy individuals BMI <24 kg/m were enrolled this...

We present a combined experimental and theoretical study of the detailed fragmentation process CO23+→ CO2+ + O+ induced by an intense laser field. Through multicoincidence fragment measurements together with ab initio molecular dynamics (AIMD) simulations, we find that transient deformation route appears in competition expected Coulomb explosion. The AIMD simulations visually demonstrate CO23+ undergoes several bending vibrations ∼50–480 fs, final dissociation stages, electron density...

The amplitude and phase of a photoionization channel provide deep insights into the nature ionization dynamics. Here, we propose novel experimental approach to retrieve these parameters from photoelectron momentum distribution (PMD), where 400 nm femtosecond laser is used ionize polarized Rb atoms prepared in $5p$ state. magnetic quantum numbers are well controlled by breaking down symmetry cooling magneto-optical trap reaction microscope. From tilt angle PMD interference structure...

Tubulin is known to undergo unique post-translational modifications (PTMs), such as detyrosination and polyglutamylation, particularly in the unstructured carboxy-terminal tails (CTTs). However, more conventional PTMs of tubulin their roles regulation microtubule properties functions remain poorly defined. Here, we report comprehensive profiling phosphorylation, acetylation, ubiquitylation, O-GlcNAcylation HeLa cells with a proteomic approach. Our tubulin-targeted analysis has identified 80...

BACKGROUND The objective of this study was to the anti-inflammatory effect and possibly involved molecular mechanisms matrine on oxidized low-density lipoprotein (ox-LDL)-exposed macrophages. MATERIAL AND METHODS Cultured human macrophages (THP-1 cell line) were exposed ox-LDL at final concentrations 0, 25, 50, 100 μg/mL. Several cells then treated with serial diluted concentrations. 2,7-Dichlorodi-hydrofluorescein diacetate (DCFH-DA) staining used evaluate reactive oxygen species (ROS)...

The analysis of the subcellular localization and function dense granule proteins (GRAs) is central importance for understanding host-parasite interaction pathogenesis Toxoplasma gondii infection. Here, we identified 15 novel GRAs used C-terminal endogenous gene tagging to determine their at intravacuolar network (IVN), parasitophorous vacuole (PV), or PV membrane (PVM) in tachyzoites periphery bradyzoites-containing cysts. functions gra genes were examined type I RH strain 5 these also...

The Zinc finger protein (ZFP) family is widely distributed in eukaryotes and interacts with DNA, RNA, various proteins to participate many molecular processes. In the present study, biological functions of eight ZFP genes lytic cycle pathogenicity Toxoplasma gondii were examined using CRISPR-Cas9 system. Immunofluorescence showed that four ZFPs (RH248270-HA, RH255310-HA, RH309200-HA, RH236640-HA) localized cytoplasm, one (RH273150-HA) was located nucleus, while expression level RH285190-HA,...

We perform high-resolution measurements of momentum distribution on ${\mathrm{Rb}}^{n+}$ recoil ions up to charge state $n=4$, where laser-cooled rubidium atoms are ionized by femtosecond elliptically polarized lasers with the pulse duration 35 fs and intensity $3.3\ifmmode\times\else\texttimes\fi{}{10}^{15}\phantom{\rule{0.16em}{0ex}}\mathrm{W}/\mathrm{c}{\mathrm{m}}^{2}$ in over-barrier ionization (OBI) regime. The distributions found exhibit multiband structures as ellipticity varies from...

We developed a magneto-optical trap reaction microscope (MOTREMI) for strontium atoms by combining multiparticle coincident detection with the laser cooling technique. The present compact injection system enables production of cold Sr in three modes two-dimensional (2D) (MOT) beam, molasses, and three-dimensional (3D) MOT, providing adjustable densities ratios ground state $5{s}^{2}$ ($^{1}S_{0}$) excited states $5s5p{\phantom{\rule{4pt}{0ex}}}^{1}{P}_{1}$ $^{3}P_{J}$. target profiles...

&lt;b&gt;&lt;i&gt;Introduction:&lt;/i&gt;&lt;/b&gt; Adipose tissue deposited on the viscera is main culprit in development of insulin resistance (IR) and cardiometabolic diseases, whereas subcutaneous adipose may have a protective role. This study aimed to propose new predictive index – visceral-fat area (VFA)-to-hip-circumference ratio (VHR) explore its efficacy for prediction IR Chinese population with type 2 diabetes mellitus. &lt;b&gt;&lt;i&gt;Methods:&lt;/i&gt;&lt;/b&gt; A total 643...

The study aimed to investigate the effect of extract Ginkgo biloba (EGb) on expression vascular endothelial growth factor (VEGF) after subarachnoid hemorrhage (SAH). Wistar rats were divided into non-SAH, SAH, vehicle, EGb1 (low-dose), and EGb2 (high-dose) groups. VEGF mRNA protein measured from brain tissues. expressions in SAH vehicle groups enhanced 24 72 hr establishment SAH. Increased positive cells found tissues further increased by pretreatment EGb. We concluded that EGb exerts...

Podoplanin (PDPN) is an important positive regulator of platelet aggregation and functions as a lymphatic endothelial marker. PDPN has been observed to be expressed in human tumor tissues various cancer cell lines. In the present study, expression patients with primary osteosarcoma was assessed at mRNA protein levels, associations between pulmonary metastasis (PM) prognosis were examined. Reverse transcription-quantitative PCR (RT-qPCR) analysis used detect levels paired normal bone (n=20...

We report on the design and capabilities of a reaction microscope (REMI) end-station at Shanghai Soft X-ray Free-Electron Laser Facility (SXFEL). This apparatus allows high-resolution 4π solid-angle coincidence detection ions electrons. The components REMI, including supersonic gas injection system, spectrometer, detectors data acquisition are described in detail. By measuring time flight impact positions electrons corresponding detectors, three-dimensional momentum vectors can be...

The photoion-photoion coincidence (PIPICO) is a simple and effective approach for the selection of correlated fragments in specific dissociating channel molecules. We propose here charge-encoded multi-photoion (cMUPICO) method, analogy to traditional PIPICO, however which charge individual taken into account. cMUPICO method allows clearly displaying coincident channels dissociation containing three more with unequal states, invisible PIPICO. As demonstration, three-body fragmentation...

Multi-ionization and subsequent Coulomb explosion (CE) of the N2O molecule irradiated by a linearly polarized 800 nm laser field is investigated reaction microscope, where number CE channels N2Oq+ with q ≤ 5 for two-body fragmentation 8 three-body were observed. For CE, analyzing internuclear separations extracted from kinetic energy releases (KERs), dissociation branching fractions, intensity dependence, interestingly, we found that N2O5+ → N3+ + NO2+ produced directly dissociating N2O3+...

This paper presents a virtual reality training system for the lower limb rehabilitation robot, which can simulate bike riding and encourage patients to join in recovery through built-in competitive game. The is variable speed active under constraint trajectory, it has adapting posture function provide individual track according legs length of patients. movement synchronization between robot model achieved by interaction control strategy, change velocity based on signal from feedback terrains...

Tpx3Cam is a newly developed time-stamped camera. With the ability to record arrival time and position of each event simultaneously, it becomes powerful tool in atomic molecular research. As an inherent weakness, time-walk effect resulting deviation major obstacle improving experimental resolution reconstruction momentum along time-of-flight. We analytic expression describe correct effect, which independent different fragments once parameters spectrometer, microchannel plate (MCP), phosphor...

The success of rapid industrialization around the world may be due to automated processes leading faster and cheaper production methods.Over past three decades, method mixing pouring concrete on site has been gradually replaced by prefabricated construction techniques in some developed countries.The industry benefits from advanced technology, which also reduces labor requirements time, saves costs, improves project quality environmental impact.The article presents current status 3D printing...

Abstract A new multi-mode electron and ion (MEI) imaging spectrometer with two arms of VMI COLTRIMS/VMI (velocity map imaging/cold-target recoil-ion momentum spectroscopy) is designed to combine various photoelectron detection modes for experiments at Shanghai soft x-ray free-electron laser (SXFEL) facility. The can be optionally operated either both in a coincidence/covariance manner (VMI arm arm), or only photoelectron/photoion detected the high-resolution arm. simulated resolutions 30–150...