A5038128462- Chronic Lymphocytic Leukemia Research
- Immunodeficiency and Autoimmune Disorders
- Lymphoma Diagnosis and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Lysosomal Storage Disorders Research
- Chronic Disease Management Strategies
- Calcium signaling and nucleotide metabolism
- COVID-19 Clinical Research Studies
- Cystic Fibrosis Research Advances
- Childhood Cancer Survivors' Quality of Life
- Pancreatic function and diabetes
- Erythrocyte Function and Pathophysiology
- Cutaneous lymphoproliferative disorders research
- Biomedical Research and Pathophysiology
- Immune Cell Function and Interaction
- Renal Diseases and Glomerulopathies
- Blood groups and transfusion
- T-cell and B-cell Immunology
Fundación Cardiovascular de Colombia
2022-2024
Bioversity International
2023
Universidad Científica del Sur
2023
Centro de Investigación del Cáncer
2009-2022
Instituto de Investigación Biomédica de Salamanca
2012-2022
Universidad de Salamanca
2009-2022
Centro de Investigación Biomédica en Red de Cáncer
2018
Consejo Superior de Investigaciones Científicas
2011
Background Chronic lymphocytic leukemia (CLL)-like monoclonal B lymphocytosis (MBL) with (MBLhi) or without (MBLlo) absolute B-lymphocytosis precedes most CLL cases,the specific determinants for malignant progression remaining unknown. Methodology/Principal Findings For this purpose, simultaneous iFISH and molecular analysis of well-established cytogenetic alterations chromosomes 11, 12, 13, 14 17 together the pattern rearrangement IGHV genes were performed in CLL-like cells from MBL cases....
Low-count monoclonal B-cell lymphocytosis is defined by the presence of very low numbers circulating clonal B cells, usually phenotypically similar to chronic lymphocytic leukemia whose biological and clinical significance remains elusive. Herein, we re-evaluated 65/91 low-count cases (54 leukemia-like 11 non-chronic leukemia-like) followed-up for a median seven years, using high-sensitivity flow cytometry interphase fluorescence in situ hybridization. Overall, clone size significantly...
Abstract Background: Monoclonal B‐cell lymphocytosis (MBL) indicates <5 × 10 9 peripheral blood (PB) clonal B‐cells/L in healthy individuals. In most cases, MBL cells show similar phenotypic/genetic features to chronic lymphocytic leukemia cells—CLL‐like MBL—but little is known about non‐CLL‐like MBL. Methods: PB samples from 639 individuals (46% men/54% women) >40 years old (62 ± 13years) with normal lymphocyte counts (2.1 0.7 /L) were immunophenotyped using high‐sensitive flow...
Risk factors associated with monoclonal B-cell lymphocytosis (MBL), a potential precursor of chronic lymphocytic leukaemia (CLL), remain unknown.Using cross-sectional study design, we investigated demographic, medical and behavioural risk MBL. "Low-count" MBL (cases) were defined as individuals very low median absolute count clonal B-cells, identified from screening healthy the remainder classified controls. 452 completed questionnaire their general practitioner, both blind to status...
Chronic antigen-stimulation has been recurrently involved in the earlier stages of monoclonal B-cell lymphocytosis, chronic lymphocytic leukemia and other lymphoproliferative disorders. The expansion two or more clones frequently reported individuals with these conditions; potentially, such coexisting have a greater probability interaction common immunological determinants. Here, we analyzed receptor repertoire molecular profile, as well phenotypic, cytogenetic hematologic features, 228...
Reference ranges of blood-circulating leukocyte populations by, e.g., age and sex, are required for monitoring immune-cell kinetics. Most previous reports in which flow cytometry has been used to define the reference counts included a limited number donors and/or cell did not consider sex simultaneously. Moreover, other factors previously considered definition normal ranges, such as presence chronic-lymphocytic-leukemia (CLL)-like low-count monoclonal B-cell lymphocytosis (MBLlo), might also...
Monoclonal B-cell lymphocytosis (MBL) with normal lymphocyte counts is associated decreased numbers of circulating subsets.Little known about the distribution lymphoid cells and their subsets in peripheral blood (PB) subjects monoclonal (MBL). In our study, we compared absolute number PB subpopulations 95 MBL cases vs. 617 age-/sex-matched non-MBL healthy (controls), using highly sensitive flow cytometry. showed significantly reduced B-cells, at expense immature naive B-cells; addition,...
Chronic lymphocytic leukaemia (CLL) is a malignant B cell disorder characterized by its high heterogeneity. Although genomic alterations have been broadly reported, protein studies are still in their early stages. Herein, 224‐antibody microarray has employed to study the intracellular signalling pathways cohort of 14 newly diagnosed B‐CLL patients as preliminary for further investigations. Several profiles were differentially identified across cytogenetic and molecular presented samples...
Patients diagnosed with chronic lymphocytic leukemia (CLL) display a high incidence of infections due to an associated immunodeficiency that includes hypogammaglobulinemia. A higher risk has also been recently reported for high-count monoclonal B-cell lymphocytosis, while no information is available in low-count lymphocytosis. Here, we evaluated the status humoral immune system patients (n=58), as well low- (n=71) and high- (n=29) count lymphocytosis versus healthy donors (n=91). Total free...
Abstract Monoclonal B cell lymphocytosis (MBL) is now recognized as the B‐lymphocyte analogue of a monoclonal gammopathy unknown significance. MBL can be precursor chronic lymphocytic leukemia or associated with non‐Hodgkin's lymphoma. It may an autoimmune abnormality related to aging (immunosenescence). The combination available new fluorochrome‐conjugated antibody reagents, multilaser instrumentation, and improved software tools have led level multicolor analysis MBL. Presently, several...
A wide variety of immunoglobulins (Ig) is produced by the immune system thanks to different mechanisms (V(D)J recombination, somatic hypermutation, and antigen selection). The profiling Ig sequences (at both DNA peptide levels) are great relevance developing targeted vaccines or treatments for specific diseases infections. Thus, genomics proteomics techniques (such as Next-Generation Sequencing (NGS) mass spectrometry (MS)) have notably increased knowledge in sequencing serum a...
Summary An increasing body of evidence suggests the potential occurrence antigen encounter by cell origin in chronic lymphocytic leukaemia ( CLL ) and ‐like monoclonal B ‐cell lymphocytosis MBL ). However, scenario which this event might occur remains unknown. In order to gain insight into we investigated molecular, cytogenetic haematological features 223 n = 84) 139) clones with stereotyped 32) versus non‐stereotyped 191) immunoglobulin heavy chain variable region IGHV amino acid sequences....
Las neoplasias hematolinfoides son consideradas de alto costo y impacto en la calidad vida, por esta razón, el uso pruebas diagnósticas sobre todo seguimiento como cuantificación enfermedad mínima residual medible, sigue siendo una herramienta para determinar pronóstico, considerando las nuevas tecnologías importancia estandarizar procesamiento. Objetivo: estructurar serie consideraciones común acuerdo expertos, medible (EMR) cinco patologías hematológicas, contexto colombiano. Métodos: se...
Introduction. Monoclonal B-cell lymphocytosis generally precedes chronic lymphocytic leukemia, affecting about 12% of the healthy adult population. This frequency increases in relatives patients with lymphoproliferative disorders. Objective. To determine monoclonal disorders, their immunophenotypic/cytogenetic characteristics, a possible relationship infectious agents, and short-term follow-up Colombian Materials methods. Fifty adults family history disorders were studied using...
DOI: 10.1002/pmic.201500372 The characterization of Chronic Lymphocytic Leukaemia (CLL) at genomic level has been performed throughout the last decades providing a broad knowledge disease. Nevertheless, studies concerning proteins have limited in this cancer. Thus, use affinity (antibody microarrays) and MS/MS proteomic strategies allowed establishment preliminary profiles different CLL stages increasing about For more details, see article by Paula Díez et al. on page 1193.
Background: Risk factors associated with monoclonal B-cell lymphocytosis (MBL), a potential precursor of chronic lymphocytic leukaemia (CLL), remain unknown.Methods: Using cross-sectional study design, we investigated demographic, medical and behavioural risk MBL.''Low-count'' MBL (cases) were defined as individuals very low median absolute count clonal Bcells, identified from screening healthy the remainder classified controls.452 completed questionnaire their general practitioner, both...
Background: To develop prediction models for COVID-19 mortality using population data recorded in the national epidemiological surveillance system of Peru.Methods: A retrospective cohort study was conducted. The consisted confirmed and registered cases nine provinces Lima, Peru. An 80% random sample selected from population, four were constructed different strategies to select variables: 1) variables reported previously machine learning models; 2) according LASSO method; 3) statistical...
Monoclonal B-Cell Lymphocytosis (MBL) is part of the mature B-cell neoplasms despite actually being a pre-neoplastic condition [1], this entity, largely unstudied, affects more than 5-10% older adults age 40 and incidence increases with aging [2,3], MBL widely associated as one main risk factors for developing Chronic Lymphocytic Leukemia (CLL)/Small Cell Lymphoma (SLL), since studies Prospective cohort verified, in effect, that CLL always preceded by MBL, therefore, greater number clonal B...