ICP4, ICP0, and ICP27 are the immediate-early (IE) regulatory proteins of herpes simplex virus that have greatest effect on viral gene expression growth. Comparative analysis mutants defective in various subsets these IE genes should help elucidate how affect cellular processes. This study focuses mutant d97, which is for encoding expresses bacterial beta-galactosidase (beta-gal) from ICP0 promoter. Together with d92 (ICP4- ICP27-) ICP0-complementing cell line L7, d97 provided a unique...

Very early in infection, herpes simplex virus (HSV) expresses four immediate-early (IE) regulatory proteins, ICP4, ICP0, ICP22, and ICP27. The systematic inactivation of sets the IE proteins cis, subsequent phenotypic analysis resulting mutants, should provide insights into how these function HSV life cycle also specific macromolecular events that are altered or perturbed cells infected with strains blocked very infection. This approach may a rational basis to assess efficacy safety mutants...

Serum lipids and the ratios are known to be associated with cardiovascular diseases (CVD). However, associations of serum related arterial stiffness unclear. We sought compare strength these assessing by brachial-ankle pulse wave velocity (baPWV) in middle-aged elderly Chinese subjects. A total number 1133 aged from 50 90 years old were recruited Shanghai downtown district. The lipids, baPWV major risk factors participants measured. Participants high exhibited higher levels non-HDL-c,...

Osteoarthritis (OA) is a common joint disorder, and the restoration of impaired cartilage remains main concern for researchers clinicians. MicroRNAs (miRNAs or miRs) play crucial roles in pathogenesis OA. The present study examined therapeutic efficacy exosomal‑miR‑140‑5p treatment OA using dental pulp stem cells (DPSCs). findings indicated that exosomal burden miR‑140‑5p was substantially increased following transfection DPSCs with mimic. administration DPSC‑derived exosomes promoted...

The cytotoxic activity of curcumin towards CCRF-CEM human T-cell leukemia cells was measured by the MTT assay. Tumor were more sensitive to or curcumin-Cu (II)compared normal cells, and IC50 ofcurcumin 8.68 µM, that ofcurcumin-Cu (II) 8.14 µM. cell cycle distribution curcumin-treated analyzed flow cytometry. DNA damage induced oxidants such as ions is considered one main causes inactivation. Therefore, we effect on agarose gel electrophoresis atomic force microscopy (AFM). Gel analyses...

Abstract Previous studies have reported that visfatin can regulate macrophage polarisation, which has been demonstrated to participate in cardiac remodelling. The aims of this study were investigate whether participates transverse aortic constriction (TAC)‐induced remodelling by regulating polarisation. First, TAC surgery and angiotensin II (Ang II) infusion used establish a mouse model, expression was measured, the results showed or Ang increased serum heart mice, phenylephrine hydrogen...

Arterial calcification is associated with cardiovascular disease as a complication of advanced atherosclerosis and significant contributor to morbidity mortality. Osteoblastic differentiation vascular smooth muscle cells (VSMCs) plays an important role in arterial characterized by cellular necrosis, inflammation, lipoprotein phospholipid complexes, especially atherosclerotic calcification. The conditioned medium from bone marrow-derived mesenchymal stem (MSC-CM) well known rich source...

Type 2 diabetes mellitus (T2DM) is a recognized trigger factor for heart failure with preserved ejection fraction (HFpEF). Recent studies show that higher serum calcium level associated greater risk of both T2DM and failure. We speculate increased related to HFpEF prevalence in patients T2DM. In this cross-sectional echocardiographic study, 807 normocalcemia normophosphatemia participated, whom 106 had HFpEF. Multinomial logistic regression was carried out determine the variables The...

Left ventricular hypertrophy (LVH) is prevalent in patients with type 2 diabetes mellitus (T2DM). Recent studies show that an increase albumin-adjusted serum calcium level associated elevated risk of T2DM. We speculate increased levels T2DM are related to LVH prevalence. In this echocardiographic study, 833 normocalcemia and normophosphatemia were enrolled. The associations between metabolic parameters, left mass index (LVMI), as well the rate examined using bivariate linear correlation,...

Aims. Left ventricular hypertrophy (LVH) and albuminuria are both markers for cardiovascular diseases (CVDs) in patients with type 2 diabetes mellitus (T2DM). We speculate that T2DM early diabetic kidney disease (DKD) could predict LVH. Methods. 333 (219 non-DKD 114 DKD) were enrolled. The association between LVMI was examined using multivariate linear regression logistic regression. Results. rate of LVH significantly higher DKD versus those without (57.0% 32.9%;<mml:math...

Cardiac autonomic neuropathy (CAN) is a common complication of type 2 diabetes mellitus (T2DM). Brachial-ankle pulse wave velocity (baPWV) known to be good surrogate marker vascular damages. The goal this study was investigate the relationship between BaPWV and CAN in T2DM.A total 148 patients who had no apparent history cardiovascular condition were enrolled consecutively study. correlation increased baPWV analyzed. evaluated by five standard reflex tests (CARTs) according Ewing's protocol:...

Interleukin-5 (IL-5) has been reported to be involved in cardiovascular diseases, such as atherosclerosis and cardiac injury. This study aimed investigate the effects of IL-5 on remodelling. Mice were infused with angiotensin II (Ang II), expression source analysed. The results showed that was time- dose-dependently decreased after Ang infusion, mainly derived from macrophages. Additionally, IL-5-knockout (IL-5-/-) mice used observe knockout II-induced We found significantly increased...

The cytotoxic activity of isoliquiritigenin (ISL) towards CCRF‐CEM human T-cell leukemia cells and DNA damage induction were investigated in the present study. anti-proliferative effect ISL on cell line vitro was analyzed. In order to further explore underlying mechanism growth inhibition line, cycle distribution mitochondrial membrane potential (ΔΨm) disruption measured. exerted significant cells. inhibitory ratio concentration- time-dependent with an IC50 value 18.38 µM. arrested G2/M...

To explore the role of miR-223 and miR-126 in predicting treatment responses to dual antiplatelet therapy (DAPT) patients with ST-elevation myocardial infarction (STEMI).Plasma levels were measured before treatment. Treatment 2-year survival determined. In vitro experiments performed mechanism action.Patients resistance DAPT had a lower level miR-126. Cardiac-event-free was shorter or levels. MiR-223 independently predicted resistance. Modulating platelets significantly changed response...

Background. Visfatin is an adipocytokine that has been demonstrated to be involved in cardiovascular diseases. This study aims at determining the role of visfatin sepsis-induced cardiac injury and identify its possible mechanisms. Methods. Dynamic changes expression mice with lipopolysaccharide- (LPS-) induced septicemia were measured. Additionally, pretreated further administered LPS observe effects on injury. Finally, septic also JSH-23 investigate whether regulates via NF-κB p65 pathway....

Grb2-associated binding protein 1 (Gab1), an intracellular scaffolding adaptor, was involved in several cardiovascular diseases. However, the role of Gab1 doxorubicin (DOX)-induced cardiotoxicity remains largely unknown. The present study investigated whether protected against DOX-induced and underlying mechanism. We overexpressed hearts using adeno-associated virus 9 system through tail vein injection. C57BL/6 mice were subjected to DOX (15 mg/kg/d, i.p.) generate cardiotoxicity....

We assessed the feasibility of spectral CT imaging for diagnosing lung adenocarcinomas manifesting as ground-glass nodules (GGNs) with varying Ki-67 expression.Spectral parameters in 116 patients GGNs were analyzed. Cases grouped into pre-invasive/minimally invasive adenocarcinoma (pre/MIA) and (IA) groups. The labeling index (Ki-67 LI) was measured compared between two LI divided three grades based on number positive-stained cells. Spectral (diameter, water, iodine concentrations lesion...

Few studies about lung ground-glass nodules (GGNs) have been done using non-enhancement spectral computed tomography (CT) imaging.To examine the association between CT parameters, Ki-67 expression, and invasiveness in adenocarcinoma manifesting as GGNs.Spectral parameters were analyzed 106 patients with GGNs. The labeling index (Ki-67 LI) was measured, divided into low expression high groups according to number of positive-stained cells (low ≤10%; >10%). Spectral compared groups. correlation...

Objective: To analyze the prevalence and clinical features of familial hypercholesterolemia (FH) in Chinese patients with myocardial infarction (MI). Method: This retrospective study recruited a total 2 119 consecutive (age (56.7±10.9) years old) undergoing coronary angiography first MI from April 2011 to December 2016. Patients were divided into groups: premature (male<55 old, female<60 non-premature MI. The diagnosis FH was established according Dutch Lipid Clinic Network (DLCN) diagnostic...

Resistin is closely related to cardiovascular diseases, and this study aimed at examining the role of resistin in doxorubicin- (DOX-) induced cardiac injury. First, 48 mice were divided into 2 groups treated with saline or DOX, expression different time points was examined (N = 24). A total 40 pretreated antiresistin neutralizing antibody (nAb) isotype IgG for 1 hour further administered DOX 5 days. The 4 groups: saline-IgG, saline-nAb, DOX-IgG, DOX-nAb 10). Cardiac injury, cardiomyocyte...

Abstract Interleukin-5 (IL-5) was reported to participate in cardiovascular diseases, this study aimed investigate the effects of IL-5 on cardiac remodeling. Mice were infused with angiotensin II (Ang II), and we found expression time- dose-dependently decreased after Ang infusion, mainly derived from macrophages. Additionally, knockout II-induced remodeling observed, results exhibited that deficiency significantly increased hypertrophy markers, elevated myocardial cell cross-sectional...