A5085309009- Genetic and phenotypic traits in livestock
- Genetic Mapping and Diversity in Plants and Animals
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Animal Genetics and Reproduction
- Chromosomal and Genetic Variations
- Veterinary Equine Medical Research
- melanin and skin pigmentation
- Photoreceptor and optogenetics research
- Anesthesia and Sedative Agents
- Diphtheria, Corynebacterium, and Tetanus
- Retinal Development and Disorders
- Animal Behavior and Welfare Studies
- Reproductive Physiology in Livestock
- Neuroscience and Neuropharmacology Research
- Nausea and vomiting management
- Hearing, Cochlea, Tinnitus, Genetics
- Genetic diversity and population structure
- Family and Disability Support Research
- Gynecological conditions and treatments
- Muscle metabolism and nutrition
- Genomics and Phylogenetic Studies
- Reproductive System and Pregnancy
- Connexins and lens biology
- Cancer-related molecular mechanisms research
- Mechanical Circulatory Support Devices
University of California, Davis
2019-2023
University of Minnesota
2015-2019
Texas A&M University
2010-2015
Mitchell Institute
2013
Oregon State University
2012
Institute of Molecular and Cell Biology
2012
National Cancer Institute
2012
Veterinary Research Institute
2012
Summary Male‐to‐female 64,XY sex reversal is a frequently reported chromosome abnormality in horses. Despite this, the molecular causes of condition are as yet poorly understood. This partially because only limited information available for horse Y (ECAY). Here, we used recently developed ECAY map and carried out first comprehensive study XY mares ( n = 18). The integrity females was studied by FISH PCR using markers evenly distributed along euchromatic region. results showed that horses has...
Background Ponies are highly susceptible to metabolic derangements including hyperinsulinemia, insulin resistance, and adiposity. Hypothesis/Objectives Genetic loci affecting height in ponies have pleiotropic effects on pathways increase the susceptibility equine syndrome (EMS). Animals Two hundred ninety‐four Welsh 529 horses. Methods Retrospective study of horses phenotyped for traits. Correlations between traits were assessed by Pearson's correlation coefficients. Complementary...
Genome analysis of the alpaca (Lama pacos, LPA) has progressed slowly compared to other domestic species. Here, we report development first comprehensive whole-genome integrated cytogenetic map for using fluorescence in situ hybridization (FISH) and CHORI-246 BAC library clones. The is comprised 230 linearly ordered markers distributed among all 36 autosomes sex chromosomes. For time, were assigned LPA14, 21, 22, 28, 36. Additionally, 86 genes from 15 chromosomes mapped dromedary camel...
Selective breeding for athletic performance in various disciplines has resulted population stratification within the American Quarter Horse (QH) breed. The goals of this study were to utilize high density genotype data to: 1) identify genomic regions undergoing positive selection and among QH subpopulations; 2) investigate haplotype structure each subpopulation; 3) candidate genes interest (ROI), as well biological pathways, predicted play a role elite group. For that, 65K SNP genotyping on...
Cytogenetic chromosome maps offer molecular tools for genome analysis and clinical cytogenetics are of particular importance species with difficult karyotypes, such as camelids (2n = 74). Building on the available human–camel zoo-fluorescence in situ hybridization (FISH) data, we developed first cytogenetic map alpaca (Lama pacos, LPA) by isolating identifying 151 bacterial artificial (BAC) clones corresponding to 44 specific genes. The genes were mapped FISH 31 autosomes sex chromosomes; 11...
The only known genetic cause of congenital stationary night blindness (CSNB) in horses is a 1378 bp insertion TRPM1. However, an affected Tennessee Walking Horse was found to have no copies this variant.To identify the for CSNB Horse.Case report detailing whole-genome sequencing (WGS) approach causal variant.A complete ophthalmic exam, including electroretinogram (ERG), performed on suspected CSNB-affected horse. WGS data were generated from case and compared with seven other breeds (n =...
Following the successful creation of a biobank from two adult Thoroughbred mares, this study aimed to recapitulate sample collection in stallions as part Functional Annotation Animal Genome (FAANG) initiative. Both underwent thorough physical, lameness, neurologic, and ophthalmic (including electroretinography) examinations prior humane euthanasia. Epididymal sperm was recovered both immediately postmortem cryopreserved. Aseptically collected full thickness skin biopsies were used isolate,...
Since domestication, horses have been selectively bred for various coat colors and white spotting patterns. To investigate breed distribution, allele frequencies, potential lethal variants recommendations on genetic testing, 29 within 14 genes were investigated in 11,281 from 28 breeds. The recessive chestnut ea melanocortin 1 receptor (MC1R) (p.D84N) was identified four breeds: Knabstrupper, Paint Horse, Percheron, Quarter Horse. After filtering relatedness, frequency Knabstruppers...
Contents Balanced autosomal translocations are a known cause for repeated early embryonic loss ( REEL ) in horses. In most cases, carriers of such phenotypically normal, but the chromosomal aberration negatively affects gametogenesis giving rise to both genetically balanced and unbalanced gametes. The latter, if involved fertilization, result , whereas gametes with form translocation will pass defect into next generation. Therefore, order reduce incidence identification is critical. Here, we...
Abstract Congenital stationary night blindness (CSNB) is an ocular disorder characterized by nyctalopia. An autosomal recessive missense mutation in glutamate metabotropic receptor 6 ( GRM6 c.533C>T, p.(Thr178Met)), called CSNB2 , was previously identified one Tennessee Walking Horse and predicted to reduce binding affinity of the neurotransmitter glutamate, impacting retinal rod ON‐bipolar cell signaling pathway. Thus, first aim identify allele frequency (AF) breeds with reported cases...
Abstract Background Distichiasis, an ocular disorder in which aberrant cilia (eyelashes) grow from the opening of Meibomian glands eyelid, has been reported Friesian horses. These misplaced can cause discomfort, chronic keratitis, and corneal ulceration, potentially impacting vision due to fibrosis, or, if secondary infection occurs, may lead loss eye. horses represent vast majority cases equine distichiasis, as breed is known be affected with inherited monogenic disorders, this condition...
The pseudoautosomal region (PAR) has important biological functions in spermatogenesis, male fertility and early development. Even though pig (Sus scrofa, SSC) is an agriculturally biomedically species, its genome sequenced, current knowledge about the porcine PAR sparse. Here we defined SSCXp/Yp by demarcating sequence of boundary at X:6,743,567 bp intron 3-4 SHROOM2 showed that truncated SSCY. Cytogenetic mapping 20 BAC clones containing 15 X-specific genes revealed largely collinear with...
A 4-year-old female alpaca (Lama pacos [LPA]) was presented to the Oregon State Veterinary Teaching Hospital for failure display receptive behavior males. Although no abnormalities were found on physical examination, transrectal ultrasonographic examination of reproductive tract revealed uterine hypoplasia and ovarian dysgenesis. Cytogenetic analysis demonstrated a normal 74,XX karyotype with 1 exceptionally small (minute) homologue autosome LPA36. Chromosome by Giemsa staining DAPI-...
American Standardbreds were developed as a harness racing horse breed. The United States Trotting Association closed the studbook in 1973 and implemented book size cap 2009. This study aimed to investigate genetic diversity Standardbred after was introduced using short tandem repeats (STRs) single-nucleotide polymorphisms (SNPs). Sixteen STRs from horses foaled 2010 2015 their sires dams (n = 50 621) utilized examine allelic richness (Ar), expected heterozygosity (HE), observed (HO),...
Abstract A growing literature supports the feasibility and validity of telehealth-based assessments for autism spectrum disorder (ASD). Better understanding families’ experiences is crucial sustained use beyond COVID-19 pandemic. This study qualitatively examines caregiver with Telehealth Evaluation Development Infants (TEDI) protocol to better understand benefits challenges evaluations. Caregivers ( N = 32) completed an online survey following a evaluation their 6–12 month-old infants....
Coat color is a trait of economic significance in horses. Variants seven genes have been documented to cause white patterning Of the 34 variants that identified KIT proto-oncogene, receptor tyrosine kinase (KIT), 27 only reported single individual or family and thus not all are routinely offered for genetic testing. Therefore, enable proper use marker-assisted selection, determining breed specificity these alleles warranted. Screening 19 unregistered all-white Shetland ponies 16 markers 14...
Polymelia, the manifestation of extra limbs or parts limbs, occurs when there is a developmental duplication in novel NHL repeat domain containing protein (NHLRC2) gene Angus cattle. In Holstein cattle, little known about etiology polymelia. To determine genetic polymelia calf, genotyping and sequencing locus, karyotyping, genome-wide association analysis were performed. The compared calf (case) to 2,650 control (normal) calves using EMMAX-GRM (Efficient Mixed-Model Association...
Structural chromosome abnormalities, such as translocations and inversions occasionally occur in all livestock species are typically associated with reproductive developmental disorders. Curiously, only a few structural aberrations have been reported camelids, most involved sex chromosomes. This can be attributed to high diploid number (2n = 74) complex morphology, which makes unambiguous identification of camelid chromosomes difficult. Additionally, molecular tools for cytogenetics sparse...
Auditory loss has been reported in camelids using brainstem auditory evoked responses (BAER). Differentiation between conductive versus sensorineural dysfunction not investigated. Therefore, the objective of study was to investigate function BAER and bone conduction (BC). Twenty-four alpacas: 15 females, 9 intact males (2-16 years age) were included a randomized clinical trial. BC recorded two derivations (vertex mastoid vertex cranial aspect second cervical vertebra). All alpacas underwent...