Antibiotic resistance (AR) is the result of microbes' natural evolution to withstand action antibiotics used against them. AR rising a high level across globe, and novel resistant strains are emerging spreading very fast. Acinetobacter baumannii multidrug Gram-negative bacteria, responsible for causing severe nosocomial infections that treated with several broad spectrum antibiotics: carbapenems, β-lactam, aminoglycosides, tetracycline, gentamicin, impanel, piperacillin, amikacin. The A....

Current study was aimed to produce nitrogen fixing Azotobacter sp. (SR-4) and phosphorus solubilizers Aspergillus niger (A. niger) evaluate their efficiency as biofertilizers for agricultural practices.Two biofertilizer including solubilizing were grown. The of determined by Kjeldahl method. Similarly, Vanadomoybdate method used measure the soluble while Heinonen analyze concentration phytase phosphatase in cultures. Furthermore, both tested a field trail on Lagenaria siceraria (bottle...

The world has revolutionized and phased into a new era, an era which upholds the true essence of technology digitalization. As market evolved at staggering scale, it is must to exploit inherit advantages opportunities, provides. With advent web 2.0, considering scalability unbounded reach that provides, detrimental for organization not adopt techniques in competitive stakes this emerging virtual set along with its advantages. transformed highly intelligent data mining approaches now allow...

Synthesis of cerium doped lanthanum phosphate nanoparticles is described here by simple co-precipitation technique. The phase, morphology and composition 2.0 mol% Ce 6.0 were analyzed Fourier transform infrared spectroscopy (FTIR) UV-visible spectroscopy. confirms the water vibration modes at a specific wave number along with group variation in numbers when incorporated to phosphate. Infrared spectral data presented for produced sample recorded on spectrometer scan range 650-4000 cm⁻¹. For...

Metal sulfide semiconductors having wide bandgap like ZnS experience a significant decrease in band gap when transition metal ions Ni2⁺ are doped. This alteration creates new composite materials with various uses photonics, solar cells, optoelectronic devices, and spintronic devices. These modifications have led to improvements nanoparticle production techniques. Nickel-doped zinc nanoparticles synthesized by using simple chemical coprecipitation method. Ni-doped been characterized...

Background: Dyslipidemia is a major risk factor for CVD, the leading cause of global morbidity and mortality. rising rapidly in low- middle-income countries like Pakistan because urbanization, sedentary lifestyles, changes diet. Determinants dyslipidemia are essential to understand prevalence development effective public health strategies, but information on its association with lifestyle factors limited Pakistani population. Objectives: The study objectives were determine examine modifiable...

The study was conducted to evaluate the effectiveness of chemical and botanical insecticides against major cabbage insects at farmer field, District Dadu, Sindh during September-December 2023. Green Light” variety sown in first week September, nursery transplanted into field October. Seven treatments were applied including control viz., 10% Tobacco leaves extract, Chinaberry fruit Neem Lambda cyhalothrin 2.5 EC, Imidacloprid 25% WP Spinosad 240 SC, replicated 3 times. An untreated plot left...

Production of extended spectrum beta -lactamases (ESBLs) by clinical isolates pathogenic E. coli is a very serious therapeutic threat. This study was aimed to investigate the prevalence ESBLs and associated drug resistance in from urine pus, report drift 2005 2009-10.Among 173 isolates, 82 were phenotypically detected as ESBL producers standard cefotaxime / clavulanic acid ceftazidime disc diffusion tests. Antimicrobial all assessed method. Presence CTX-M, TEM, SHV OXA groups investigated...

Bacillary dysentery, common in developing countries, is usually caused by Shigella species. A major problem shigellosis the rapid emergence of multidrug-resistant strains. This first detailed molecular study on drug resistance isolates from Faisalabad region Pakistan. Ninety-five obtained after screening 2500 stool samples were evaluated for vitro to commonly used antimicrobial agents; presence or absence 20 most relevant genes; and prevalence integrons 1, 2, 3. flexneri was found be...

Pseudomonas aeruginosa (P. aeruginosa) is an aerobic gram-negative, non-spore forming, rod-shaped bacterium. It accelerates the decline in lung function and ultimately leads to increased mortality morbidity rate. Survival virulence of P. due its biofilm formation ability. The main aim this study was test synergistic effect silver nanoparticles (AgNPs) combination with Polymyxin B against biofilms aeruginosa. A total 500 pus aspirations were collected bacterial pathogens identified. Biofilm...

Abstract The current study is a retrospective epidemic report regarding dengue fever (DF) virus infection cases (2017) from fifteen districts of KPK, Pakistan. Medical records 120 948 patients were reviewed retrospectively for demographic, clinical and laboratory data. presence was confirmed by NS1-ELISA RT-PCR, respectively. total positive (of suspected DF samples) 24 938 (20.6%), whereas seventy (0.28%) had fatal outcome. Mean age ± SD the 26 19.8 years, while; most affected group 16 to 30...

Klebsiella pneumoniae is ubiquitous and known to be a notorious pathogen of humans, animals, plant-based foods. K. recognized trafficker antibiotic resistance genes (ARGs) between from different ecological niches. A total 775 samples ( n = 775) were collected September 2017 August 2019 environmental sources by applying the random convenient sampling technique. 120 (15.7%) confirmed as . The distribution among environment, animals was 17.1, 12.38, 10%, respectively. Isolates have shown...

Abstract Typhoid fever is transmitted by ingestion of polluted water, contaminated food, and stool typhoid-infected individuals, mostly in developing countries with poor hygienic environments. To find novel therapeutic targets inhibitors, We employed a subtractive genomics strategy towards Salmonella Typhi the complete genomes eight strains were primarily subjected to EDGAR tool predict core genome ( n = 3207). Human non-homology 2450) was followed essential genes identification 37). The...

Abstract Shared needles are a possible iatrogenic and hematogenous inanimate vector of African Swine Fever virus (ASFV) in farm conditions. To evaluate that transmission, sixty, 4-week-old pigs were procured from an ASF free herd free. Upon arrival, randomly divided into two sets. Set 1 served as seeder pigs, allocated to 4 groups. The other 8 groups, sentinels. Seeder oronasally challenged with ASFV at high (10 copy numbers/mL), moderate 6 numbers/mL) or low challenge titer, except subgroup...

Introduction: This study aimed to determine the drug susceptibility patterns and genetic elements related resistance in isolates of Salmonella enterica serovar Typhi (S. Typhi) from Faisalabad region Pakistan. Methodology: The status 80 were evaluated by determining antimicrobial susceptibility, MICs, genes involved, presence integrons. Nalidixic acid reduced ciprofloxacin also investigated mutation screening gyrA, gyrB, parC, parE genes. Results: Forty-seven (58.7%) multidrug resistant...

Wound infections are often difficult to treat due various bacterial pathogens. Pseudomonas aeruginosa is one of the common invaders open wounds. Precise diagnosis this etiological agent in wound critical importance particularly treatment problematic cases. The existing diagnostic methods have certain limitations related specificity. Our objective was establish a comprehensive and reliable multiplex PCR confirm P. aeruginosa.A test developed for rapid identification aeruginosa. Four highly...

Exploring biological agents to control biofilm is a vital alternative in combating pathogenic bacteria that cause dental plaque. This study was focused on antimicrobial, formation and dispersal efficacy of Gallic acid (GA) against bacteria, including Proteus spp., Escherichia coli, Pseudomonas Salmonella Streptococcus mutans, Staphylococcus aureus multispecies bacteria. Biofilm qualitatively quantitatively assessed by crystal violet assay, florescence microscopy (bacterial biomass (µm2),...

The present study was investigated to evaluate the hepato-ameliorating and antioxidant activity of two aqueous extracts Solanum nigrum (SN) against CCl4-induced toxicity in rats. Male Albino rats were divided four groups with 8 animals each group. Group (1) normal group (2) injected intraperitoneal (i.p.) CCl4 (1ml/kg) 3 times weekly for 2 weeks (control). (3) then treated extract from whole plant (500 mg/kg) (4) fruits (250 mg/kg). injection enhanced hepatic enzymes (AST ALT) while it...