A5005019098- Cystic Fibrosis Research Advances
- Nanowire Synthesis and Applications
- Tissue Engineering and Regenerative Medicine
- Biosimilars and Bioanalytical Methods
- Inhalation and Respiratory Drug Delivery
- Respiratory viral infections research
- Atomic and Subatomic Physics Research
- Diamond and Carbon-based Materials Research
- Virus-based gene therapy research
- RNA Research and Splicing
- Advanced biosensing and bioanalysis techniques
- Advanced Biosensing Techniques and Applications
- Corneal Surgery and Treatments
- Nanoparticle-Based Drug Delivery
- CRISPR and Genetic Engineering
- Corneal surgery and disorders
- Antifungal resistance and susceptibility
- Neonatal Respiratory Health Research
- Glaucoma and retinal disorders
- Spacecraft and Cryogenic Technologies
- Peptidase Inhibition and Analysis
- Superconducting Materials and Applications
- RNA Interference and Gene Delivery
Regional Centre of Advanced Technologies and Materials
2024
Palacký University Olomouc
2022-2024
Institute of Molecular and Translational Medicine
2022-2024
University Hospital Olomouc
2023
Abstract The present study focuses on the development of a sensitive and specific approach for visualization sentinel lymph nodes draining tumor using ultrabright 200‐nm fluorescent nanodiamonds (FNDs) equipped with designed targeting surface architecture. FNDs narrow size distribution are isolated by differential centrifugation, colloidally stabilized alkyne‐functionalized poly(glycerol) modified polyvalent array mannose (FND‐p‐Man). In vitro experiments demonstrate an outstanding increase...
The global shortage of corneal endothelial graft tissue necessitates the exploration alternative therapeutic strategies. Rho-associated protein kinase inhibitors (ROCKi), recognized for their regenerative potential in cardiology, oncology, and neurology, have shown promise regeneration. This study investigates repurposing additional ROCKi compounds. Through screening a self-assembled library on B4G12 cells, we evaluated dose-dependent effects proliferation, migration, toxicity using...
CFTR is a membrane protein that functions as an ion channel. Mutations disrupt its biosynthesis, trafficking or function cause cystic fibrosis (CF). Here, we present novel in vitro model system prepared using CRISPR/Cas9 genome editing with endogenously expressed WT-CFTR tagged HiBiT peptide. To enable the detection of plasma live cells, inserted tag fourth extracellular loop WT-CFTR. The 11-amino acid binds high affinity to large inactive subunit (LgBiT), generating reporter luciferase...
Cystic fibrosis (CF) is the most common autosomal recessive genetic disease in Caucasians, affecting more than 100,000 individuals worldwide. It caused by pathogenic variants gene encoding CFTR , an anion channel at plasma membrane of epithelial and other cells. Many CF disrupt biosynthesis trafficking or reduce its ion function. The frequent mutation, loss a phenylalanine position 508 (F508del), leads to misfolding, retention endoplasmic reticulum, premature degradation protein....