A5074554158- MicroRNA in disease regulation
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- SARS-CoV-2 and COVID-19 Research
- RNA Research and Splicing
- COVID-19 epidemiological studies
- RNA regulation and disease
- CRISPR and Genetic Engineering
- Nuclear Receptors and Signaling
- Genomics and Chromatin Dynamics
- Animal Virus Infections Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Plant Virus Research Studies
- Extracellular vesicles in disease
- Mitochondrial Function and Pathology
- Cancer-related gene regulation
- Genetics and Neurodevelopmental Disorders
- RNA and protein synthesis mechanisms
- Histone Deacetylase Inhibitors Research
- Virology and Viral Diseases
- Protein Degradation and Inhibitors
- HVDC Systems and Fault Protection
- Immune cells in cancer
- Influenza Virus Research Studies
ETH Zurich
2019-2022
German Center for Neurodegenerative Diseases
2014-2021
University of Göttingen
2019-2021
Institute of Developmental Physiology
2019
École Polytechnique Fédérale de Lausanne
2019
ABSTRACT Aims Deregulation of epigenetic processes and aberrant gene expression are important mechanisms in heart failure. Here we studied the potential relevance m6A RNA methylation failure development. Methods results We analysed via next‐generation sequencing. found that approximately one quarter transcripts healthy mouse human exhibit methylation. During progression to observed changes exceed both human. RNAs with altered were mainly linked metabolic regulatory pathways, while level...
Aging and increased amyloid burden are major risk factors for cognitive diseases such as Alzheimer's disease (AD). Effective therapies these lacking. Here, we evaluated mouse models of age-associated memory impairment deposition to study transcriptome cell type–specific epigenome plasticity in the brain peripheral organs. We determined that aging pathology associated with inflammation impaired synaptic function hippocampal CA1 region result epigenetic-dependent alterations gene expression....
Endophilin-A, a well-characterized endocytic adaptor essential for synaptic vesicle recycling, has recently been linked to neurodegeneration. We report here that endophilin-A deficiency results in impaired movement, age-dependent ataxia, and neurodegeneration mice. Transcriptional analysis of mutant mice, complemented by proteomics, highlighted ataxia- protein-homeostasis-related genes revealed upregulation the E3-ubiquitin ligase FBXO32/atrogin-1 its transcription factor FOXO3A. FBXO32...
Aging is accompanied by gradually increasing impairment of cognitive abilities and constitutes the main risk factor neurodegenerative conditions like Alzheimer's disease. The underlying mechanisms are however not well understood. Here we analyze hippocampal transcriptome young adult mice two groups at advanced age using RNA sequencing. This approach enabled us to test differential expression coding non-coding transcripts, as splicing editing. We report a specific age-associated gene...
Kmt2a and Kmt2b are H3K4 methyltransferases of the Set1/Trithorax class. We have recently shown importance for learning memory. Here, we report that is also important in memory formation. compare decrease methylation de-regulation gene expression hippocampal neurons mice with knockdown either or Kmt2b. control largely distinct genomic regions different molecular pathways linked to neuronal plasticity. Finally, show resulting from partially recapitulates pattern previously reported CK-p25...
Small RNA molecules play important roles in many biological processes and their dysregulation or dysfunction can cause disease. The current method of choice for genome-wide sRNA expression profiling is deep sequencing. Here we present Oasis 2, which a new main release the web application detection, differential expression, classification small RNAs sequencing data. Compared to its predecessor Oasis, 2 features novel speed-optimized detection module that supports identification any organism...
Histone acetylation is essential for memory formation and its deregulation contributes to the pathogenesis of Alzheimer's disease. Thus, targeting histone discussed as a novel approach treat dementia. The landscape shaped by chromatin writer eraser proteins, while readers link state cellular function. Chromatin emerged drug targets in cancer research but little known about manipulation adult brain. Here we tested effect JQ1-a small-molecule inhibitor BRD2, BRD3, BRD4 BRDT-on brain function...
Abstract Summary: Oasis is a web application that allows for the fast and flexible online analysis of small-RNA-seq (sRNA-seq) data. It was designed end user in lab, providing an easy-to-use frontend including video tutorials, demo data best practice step-by-step guidelines on how to analyze sRNA-seq Oasis’ exclusive selling points are differential expression module multivariate samples, classification robust biomarker detection advanced programming interface supports batch submission jobs....
Genome sequences from evolving infectious pathogens allow quantification of case introductions and local transmission dynamics. We sequenced 11,357 severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genomes Switzerland in 2020-the sixth largest effort globally. Using a representative subset these data, we estimated viral to their persistence over the course 2020. contrasted estimates with simple null models representing absence certain public health measures. show that...
Increase in the size of human neocortex―acquired evolution―accounts for unique cognitive capacity humans. This expansion reflects evolutionarily enhanced proliferative ability basal progenitors (BPs), including radial glia and intermediate (bIPs) mammalian cortex, which may have been acquired through epigenetic alterations BPs. However, how epigenome BPs differs across species is not known. Here, we report that histone H3 acetylation a key regulation bIP amplification cortical expansion....
Prion diseases are fatal neurodegenerative disorders caused by misfolding of the normal prion protein into an infectious cellular pathogen. Clinically characterized rapidly progressive dementia and accounting for 85% human disease cases, sporadic Creutzfeldt-Jakob (sCJD) is prevalent disease. Although sCJD neuropathological hallmarks well-known, associated molecular alterations elusive due to rapid progression absence preclinical stages. To investigate transcriptome during progression, we...
Abstract SARS-CoV-2, the virus responsible for current COVID-19 pandemic, is evolving into different genetic variants by accumulating mutations as it spreads globally. In addition to this diversity of consensus genomes across patients, RNA viruses can also display within individual hosts, and co-existing viral may affect disease progression success medical interventions. To systematically examine intra-patient we processed a large cohort 3939 publicly-available deeply sequenced with...
The duck represents an important reservoir of influenza viruses for transmission to other avian and mammalian hosts, including humans. increased pathogenicity the recently emerging clades highly pathogenic (HPAI) H5N1 subtype in ducks features systemic viral spread organ-to-organ variation transcription tissue damage. We previously reported that experimental infection Sudani (Cairina moschata) with Egyptian HPAI (H5N1) virus (clade 2.2.1.2) high replication severe damage lung, but lower only...
Abstract Pathogen genomes provide insights into their evolution and epidemic spread. We sequenced 1,439 SARS-CoV-2 from Switzerland, representing 3-7% of all confirmed cases per week. Using these data, we demonstrate that no one lineage became dominant, pointing against towards general lower virulence. On an epidemiological level, report evidence cryptic transmission before the first case. find many early viral introductions Germany, France, Italy recent Germany France. Over summer, quantify...
Abstract Genome sequences from evolving infectious pathogens allow quantification of case introductions and local transmission dynamics. We sequenced 11,357 SARS-CoV-2 genomes Switzerland in 2020 - the 6th largest effort globally. Using a representative subset these data, we estimated viral to their persistence over course 2020. contrast estimates with simple null models representing absence certain public health measures. show that Switzerland’s border closures de-coupled incidence...
ABSTRACT Physical exercise in combination with cognitive training is known to enhance synaptic plasticity, learning & memory and lower the risk for various complex diseases including Alzheimer’s disease. Here we show that exposure of adult male mice an environmental enrichment paradigm leads enhancement plasticity cognition also next generation. We effect mediated through sperm RNA explained by microRNAs 212/132. In conclusion, our study reports intergenerational inheritance acquired...
Abstract Oasis 2 is a new main release of the web application for detection, differential expression, and classification small RNAs in deep sequencing data. Compared to its predecessor Oasis, features novel speed-optimized sRNA detection module that supports identification any organism with higher accuracy. Next improved target organism, software now also recognizes potential cross-species miRNAs viral bacterial sRNAs infected samples. In addition, can be queried visualized interactively,...