A5010940154- Fibroblast Growth Factor Research
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Eosinophilic Disorders and Syndromes
- Kruppel-like factors research
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- GDF15 and Related Biomarkers
- Cancer, Hypoxia, and Metabolism
- Monoclonal and Polyclonal Antibodies Research
- Virus-based gene therapy research
- Mast cells and histamine
- Cardiac tumors and thrombi
- Cellular transport and secretion
- Animal Virus Infections Studies
- Coronary Interventions and Diagnostics
- Nutrition and Health in Aging
- Liver Disease and Transplantation
- Antiplatelet Therapy and Cardiovascular Diseases
- Listeria monocytogenes in Food Safety
- Neurosurgical Procedures and Complications
- Circular RNAs in diseases
- Peptidase Inhibition and Analysis
- Protein Tyrosine Phosphatases
Inner Mongolia University of Science and Technology
2025
Baotou Medical College
2025
Moderna Therapeutics (United States)
2019
AVEO Oncology (United States)
2009-2013
Massachusetts Institute of Technology
2003-2012
Koch Institute for Integrative Cancer Research At MIT
2008
Center for Cancer Research
2003-2007
Beth Israel Deaconess Medical Center
2005
Harvard University
2005
The University of Texas Southwestern Medical Center
1998-2000
Influenza A virus infection is a major source of morbidity and mortality worldwide. Because the effectiveness existing vaccines antiviral drugs limited, development new treatment modalities needed. Here, we show that short interfering RNAs (siRNAs) specific for conserved regions influenza genes can prevent treat in mice. Virus production lungs infected mice reduced by siRNAs given either before or after initiating infection, using slow i.v. administration small volumes containing complexes...
Local mRNA therapy encoding inflammatory cytokines and T cell costimulator OX40L mediates persistent anticancer immunity across tumor models.
Krüppel-like factor 2 (KLF2) is a member of zinc-finger transcription factors. Based on its expression in naive and memory T cells the activated phenotype few mice lacking KLF2 lymphoid lineage, postulated to regulate cell homeostasis by promoting quiescence. In this study, we show that reporter gene assays directly activates promoters both CD62L sphingosine-1-phosphate receptor 1 (S1P1), whose critical for egress from thymus homing lymph nodes. Correspondingly, exogenous primary...
Abstract Dysregulated fibroblast growth factor (FGF) signaling has been implicated in the pathogenesis of human cancers. Aberrant activation FGF receptor 2 (FGFR2) signaling, through overexpression FGFR2 and/or its ligands, mutations, and amplification, found a variety tumors. We generated monoclonal antibodies against extracellular ligand-binding domain to address role tumorigenesis explore potential as novel therapeutic target. surveyed broad panel cancer cell lines for dysregulation...
Abstract TCR interactions with cognate peptide-MHC (pepMHC) ligands are generally low affinity. This feature, together the requirement for CD8/CD4 participation, has made it difficult to dissect relationships between TCR-binding parameters and T cell activation. Interpretations further complicated when comparing different pepMHC, because these can vary greatly in stability. To examine properties responses, this study we characterized activities mediated by a panel of TCRs that differed...
To study T cell responses to tumors in an autochthonous model, we expressed a CD8 epitope SIYRYYGL (SIY) the prostate of transgenic adenocarcinoma (TRAMP) mice (referred as TRP-SIY), which spontaneously develop cancer. Naïve SIY-specific cells adoptively transferred into TRP-SIY became tolerized draining lymph nodes. Vaccination intranasally with influenza virus that expresses SIY resulted generation effector lung-draining These TNFalpha and IFNgamma, eliminated peptide-loaded target vivo,...
To study competition between naïve and memory T cells, we examined proliferation of adoptively transferred CD8 + cells in lymphopenic recipients or containing a clonal population cells. We find hierarchy the extent cell that appears to correlate with strength receptor (TCR)-self-peptide-MHC (pepMHC) interactions. also proliferate full complement different TCR if experience stronger TCR-self-pepMHC interactions than resident Furthermore, contain TCR, but this case relative strengths are not...
Abstract A major obstacle to efficacious T cell-based cancer immunotherapy is the tolerizing-tumor microenvironment that rapidly inactivates tumor-infiltrating lymphocytes. In an autochthonous model of prostate cancer, we have previously shown intratumoral injection Ag-loaded dendritic cells (DCs) delays cell tolerance induction as well refunctionalizes already tolerized in tumor tissue. this study, defined molecular interactions mediate effects DCs. We show pretreating DCs with anti-CD70 Ab...
ABSTRACT Subclinical infection of BALB/c mice with the intracellular bacterial pathogen Listeria monocytogenes results in development protective antilisterial immunity. L. can infect hepatocytes, and cytotoxic T lymphocytes (CTL) lyse -infected hepatocytes a major histocompatibility complex (MHC) class Ia-restricted manner. It remained to be determined whether are susceptible MHC Ib-restricted cytolysis. In this study, we showed that express Ib molecule Qa-1 b mRNA protein. We further...
Abstract The class Ib molecule Qa-1b binds the Ia leader peptide, Qdm, which reacts with CD94/NKG2R on NK cells. We have generated a gene that encodes Qdm peptide covalently attached to β2-microglobulin (β2M) by flexible linker (Qa-1 determinant modifier (Qdm)-β2M). When this construct is expressed in TAP-2− or β2M− cells, it allows for expression of Qdm-β2M protein associates generate epitope, as detected Qdm/Qa-1b-specific CTL. To test biological significance engineered molecule, we...
Abstract Many leader-derived peptides require TAP for presentation by class I molecules. This dependence can either be ascribed to the inability of proteases resident in endoplasmic reticulum (ER) trim leader peptide precursors into appropriate epitope or failure a portion segment gain access lumen ER. Using Qa-1 binding epitope, Qdm derived from Ia as model, we show that many cell types lack ER protease activity this at its C terminus. However, both T1 and T2 cells contain process full...
Abstract Background: We have provided initial evidence1 on the clinical usefulness of cancer cachexia stages (CCS) proposed by Fearon et al2. However it is still unclear3 if particular molecular phenotypes are also associated with these stages, as well relevant outcomes. Methods: A candidate list cytokines (Activin A, Eotaxin, FGF, G-CSF, GDF15, GM-CSF, IFN-g, IL-10, IL-12_p70, IL-13, IL-15, IL-17, IL-1b, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IP-10, MCP-1_MCAF, MIP-1a, MIP-1b,...
Abstract Background: Cachexia is associated with increased inflammatory markers and decreased survival in cancer. Also, elevated GDF-15 has been poor prognosis several cancer types but its role cachexia not well-understood. Methods: We measured body weight change, appetite, plasma GDF-15, other 62 males cancer-cachexia (CC), 72 non-cachectic subjects (CNC) 64 non-cancer controls (Co) matched by age, gender pre-illness weight. In a subset of patients we also grip strength (HGS), appendicular...
Abstract Immunologic responses to cancer involve complex interactions of cells within the tumor microenvironment (TME). These are mediated in part through co-stimulatory and inhibitory transmembrane proteins, cytokines chemokines. Direct intratumoral (ITu) administration messenger RNA results synthesis proteins that can prime enhance an anti-cancer immune response. Priming, expansion migration T-cell clones may also result a systemic or ‘abscopal’ response distal lesions. There is mounting...
Abstract Aberrant activation of fibroblast growth factor receptor 2 (FGFR2) signaling, through overexpression FGFR2 and/or its ligands, mutations, and amplification have been found in a variety human tumors. We generated monoclonal antibodies against the extracellular ligand binding domain to address role tumorigenesis explore potential as novel therapeutic target. identified broad panel cancer cell lines with dysregulated signaling examined sensitivity these specifically targeting FGFR2....
<div>Abstract<p>Dysregulated fibroblast growth factor (FGF) signaling has been implicated in the pathogenesis of human cancers. Aberrant activation FGF receptor 2 (FGFR2) signaling, through overexpression FGFR2 and/or its ligands, mutations, and amplification, found a variety tumors. We generated monoclonal antibodies against extracellular ligand-binding domain to address role tumorigenesis explore potential as novel therapeutic target. surveyed broad panel cancer cell lines for...