A5081514177- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- Immune Response and Inflammation
- Multiple Myeloma Research and Treatments
- Virus-based gene therapy research
- Adrenal Hormones and Disorders
- Spaceflight effects on biology
- CAR-T cell therapy research
- Phytoplasmas and Hemiptera pathogens
- Plant Pathogenic Bacteria Studies
- MicroRNA in disease regulation
- Advanced biosensing and bioanalysis techniques
- Histone Deacetylase Inhibitors Research
- RNA Research and Splicing
- Cocoa and Sweet Potato Agronomy
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Cancer Immunotherapy and Biomarkers
- Diet and metabolism studies
- Diabetes and associated disorders
- Antimicrobial Peptides and Activities
- Chronic Lymphocytic Leukemia Research
- Hedgehog Signaling Pathway Studies
- Protease and Inhibitor Mechanisms
Peking University
2016-2025
Anhui University
2023
National Health and Family Planning Commission
2022-2023
Auburn University
2019-2022
China Astronaut Research and Training Center
2021
Peking University Third Hospital
2020
Bengbu Medical College
2020
Yonsei University College of Dentistry
2015-2018
Yonsei University
2015-2018
Deutsches Herzzentrum München
2015
The developmental requirements for immunological memory, a central feature of adaptive immune responses, is largely obscure. We show that as naive CD8 T cells undergo homeostasis-driven proliferation in lymphopenic mice the absence overt antigenic stimulation, they progressively acquire phenotypic and functional characteristics antigen-induced memory cells. Thus, homeostasis-induced express typical cell markers, lyse target directly vitro vivo, respond to lower doses antigen than cells,...
Influenza A virus causes widespread infection in the human respiratory tract, but existing vaccines and drug therapy are of limited value. Here we show that short interfering RNAs (siRNAs) specific for conserved regions viral genome can potently inhibit influenza production both cell lines embryonated chicken eggs. The inhibition depends on presence a functional antisense strand siRNA duplex, suggesting mRNA is target RNA interference. However, nucleocapsid (NP) or component transcriptase...
Influenza A virus infection is a major source of morbidity and mortality worldwide. Because the effectiveness existing vaccines antiviral drugs limited, development new treatment modalities needed. Here, we show that short interfering RNAs (siRNAs) specific for conserved regions influenza genes can prevent treat in mice. Virus production lungs infected mice reduced by siRNAs given either before or after initiating infection, using slow i.v. administration small volumes containing complexes...
High-molecular-mass polyethylenimines (PEIs) are widely used vectors for nucleic acid delivery. We found that removal of the residual N -acyl moieties from commercial linear 25-kDa PEI enhanced its plasmid DNA delivery efficiency 21 times in vitro , as well 10,000 mice with a concomitant 1,500-fold enhancement lung specificity. Several additional PEIs were synthesized by acid-catalyzed hydrolysis poly(2-ethyl-2-oxazoline), yielding pure polycations. PEI87 and PEI217 exhibited highest :...
Current nonviral genetic vaccine systems are less effective than viral vaccines, particularly in cancer where epitopes can be weakly immunogenic and antigen-presenting cell processing presentation to T cells is down-regulated. A promising delivery method for vaccines involves microencapsulation of antigen-encoding DNA, because such particles protect plasmid payloads target them phagocytic cells. However, conventional microparticle formulations composed poly lactic-co-glycolic acid take too...
<b><i>Background: </i></b> Generalized epilepsy with febrile seizures plus (GEFS+) is an autosomal dominant syndrome characterized by (FS) and a variety of afebrile generalized seizure types. GEFS+ has previously been linked to mutations in two genes encoding the voltage-gated sodium channel α-subunit (<i>SCN1A</i>) β1-subunit (<i>SCN1B</i>). We studied large family FS partial as well <b><i>Methods: All but living affected members were interviewed examined. Information on deceased was...
Background— ADAMTS-7, a member of the disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) family, was recently identified to be significantly associated genomewide coronary artery disease. However, mechanisms that link ADAMTS-7 disease risk remain elusive. We have previously demonstrated promotes vascular smooth muscle cell migration postinjury neointima formation via degradation matrix protein cartilage oligomeric protein. Because delayed endothelium repair renders...
Factors that affect naïve T cell proliferation in syngeneic lymphopenic hosts were investigated. 2C receptor (TCR) transgenic cells lacking both CD8 and CD4 survived but hardly proliferated. Proliferation of + was proportional to the abundance cognate peptide/MHC complexes severely inhibited by injection anti-CD8 antibody. Weakly reactive self-peptides slightly enhanced whereas a potent agonist peptide promoted much more rapid proliferation, inflammation-stimulating adjuvant had only small...
Following transfer into lymphopenic hosts, naive CD8 T cells proliferate and acquire memory phenotype. Although the acquired phenotype is stable in recombination activating gene-1-deficient (RAG −/− ) recipients, sublethally irradiated mice of donor origin gradually accumulate. The have been attributed to phenotypic reversion homeostatic cells, implying instability restoration cell compartment by homeostasis-driven proliferation. We show here that ( i accumulation only occurs recipients an...
Abstract Interleukin (IL)-27, a newly discovered IL-12 family cytokine, is composed of p28 and EBI3. In this study, CD11c-p28 f/f conditional knockout mice were generated to delete specifically in dendritic cells (DCs). We demonstrated that the absence DC-derived p28, these highly susceptible both low higher concentrations concanavalin A (ConA) (5 mg/kg or 10 mg/kg), with extremely early steady high levels interferon-γ (IFN-γ) sera. Neutralizing IFN-γ prevented ConA-induced liver damage...
ABSTRACT Peptide-conjugated phosphorodiamidate morpholino oligomers (P-PMO) are single-stranded nucleic acid-like antisense agents that can reduce gene expression by sterically blocking complementary RNA sequence. P-PMO water soluble and nuclease resistant, they readily achieve uptake into cells in culture under standard conditions. Eight P-PMO, each 20 to 22 bases length, were evaluated for their ability inhibit influenza A virus (FLUAV) A/PR/8/34 (H1N1) replication cell culture. The...
Small hairpin RNAs (shRNAs) are widely used in RNAi studies and typically consist of a stem 19–29 base pairs (bp), loop at least 4 nucleotides (nt), dinucleotide overhang the 3′ end. Compared with shRNAs 21–29 bp stems, we have found that 19-bp or shorter stems (sshRNAs) possess some unique structure–activity features depend on whether antisense strand is positioned 5′ to (L- R-type sshRNAs, respectively). L sshRNAs can IC 50 s very low picomolar range, nominal sizes 1 nt were as active...
Exposure to microgravity leads alterations in multiple systems, but microgravity-related changes the gastrointestinal tract and its clinical significance have not been well studied. We used hindlimb unloading (HU) mouse model simulate a condition investigated intestinal microbiota colonic epithelial cells. Compared with ground-based controls (Ctrls), HU affected fecal composition profile that was characterized by expansion of Firmicutes decrease Bacteroidetes. The colon epithelium mice...
The immune systems can be altered by spaceflight in many aspects, but microgravity-related mucosal changes and its clinical significance have not been well studied. purpose of this study was to investigate whether simulated microgravity influences the intestinal homeostasis increases susceptibility colon inflammation. hindlimb unloading (HU) mouse model used simulate condition. Three percent dextran sulfate sodium (DSS) given mice induce colitis. Compared ground control (Ctrl) mice, HU ones...
Abstract Background The characteristics of neutralizing antibodies (NAbs) and antibody against major antigen proteins related to clinical outcomes in severe coronavirus disease 2019 (COVID-19) patients were still less known. Methods NAbs targeting nucleocapsid (N), spike protein (S), the receptor-binding domain (RBD) longitudinal plasma samples from LOTUS China trial measured by microneutralization assay enzyme-linked immunosorbent (ELISA). Viral load was determined real-time reverse...
Naive T cells do not proliferate in normal individuals the absence of antigen stimulation, but they spontaneously when are severely depleted. We show here that coculture syngeneic dendritic (DC) with naive expressing a single cell receptor also results proliferation foreign antigen. As lymphopenic mice, where depends upon DC, this response system requires interaction cells' self-peptide-MHCs on DC. This vitro soluble factors, including IL-15 secreted by and can be inhibited potently...
Small hairpin RNAs (shRNAs) with 19-base-pair, or shorter, stems (short shRNAs [sshRNAs]) have been found to constitute a class whose mechanism of action appears be distinct from that small interfering (siRNAs) longer shRNAs. These sshRNAs can as active canonical siRNAs Their activity is affected by whether the antisense strand positioned 5′ 3′ loop (L R sshRNAs, respectively). Dicer seems not involved in processing although target gene suppression these hairpins through Ago2-mediated mRNA...
// Liang Lin 1, * , Fan Yan 3, Dandan Zhao 4, Meng Lv 2 Xiaodong 5 Hui Dai 1 Xiaodan Qin Zhang Jie Hao Xiuyuan Sun Yanhui Yin Xiaojun Huang Jun Jin Lu Qing Ge Key Laboratory of Medical Immunology, Ministry Health, Department School Basic Sciences, Peking University Health Science Center, Beijing 100191, China Institute Hematology, People's Hospital, 100044, 3 Tianjin Cancer and 300060, 4 Jining No.1 Jining, Shandong 272011, Hangzhou Hang Zhou 310002, These authors have contributed equally to...
Abstract Prostaglandins E2 (PGE2) has been shown to enhance IgE production by B cells in vitro . The physiological and pathological relevance of this phenomenon the underlying molecular mechanism, however, remain be elucidated. from wild type EP2-deficient mice were compared culture for their responses PGE2 terms class switching production. Ovalbumin (OVA)-induced asthma models used evaluate impact EP2-deficiency on development asthma. promoted switching, generation + secretion stimulated...
Exposure to space environment induces alterations in glucose and lipid metabolism that contribute muscular atrophy, bone loss, cardiovascular disorders. Intestinal microbiota is also changed, but its impact on spaceflight-related metabolic disorder not clear. We investigated the relationship between changes gut dysbiosis a hind limb-unloading (HU) mouse model, well-accepted ground-based spaceflight analog. Impaired body weight gain, intolerance, peripheral insulin resistance were found...
Dysregulated mucosal immunity plays an essential role in the pathophysiology of inflammatory bowel disease (IBD). Transient receptor potential vanilloid 1 (TRPV1) is a Ca2+-permeable ion channel that implicated modulating immune responses. However, its pathogenesis intestinal inflammation remains elusive. Here, we found TRPV1 gain function significantly increased susceptibility mice to experimental colitis, and was associated with excessive recruitment dendritic cells enhanced Th17 responses...