A5101979513- Hepatitis C virus research
- Neuroinflammation and Neurodegeneration Mechanisms
- Medical and Biological Ozone Research
- interferon and immune responses
- Hepatitis B Virus Studies
- Spaceflight effects on biology
- Climate Change and Health Impacts
- Tryptophan and brain disorders
- SARS-CoV-2 and COVID-19 Research
- Immune Cell Function and Interaction
- HIV Research and Treatment
- Neurological Disease Mechanisms and Treatments
- Liver Disease Diagnosis and Treatment
- Atherosclerosis and Cardiovascular Diseases
- Herpesvirus Infections and Treatments
- Barrier Structure and Function Studies
- Immune cells in cancer
- Renal Transplantation Outcomes and Treatments
- Stress Responses and Cortisol
- Liver Disease and Transplantation
- Neurogenesis and neuroplasticity mechanisms
- Alzheimer's disease research and treatments
- Viral Infections and Vectors
- Hedgehog Signaling Pathway Studies
- Medical Research and Treatments
Second Military Medical University
2022-2023
Changhai Hospital
2022-2023
Jiangnan University
2023
University of Southern California
2023
Qingdao University
2022
Wuhan University
2009-2022
Affiliated Hospital of Qingdao University
2022
Guangxi Zhuang Autonomous Region Health and Family Planning
2020
Temple University
2010-2015
Shaanxi Normal University
2015
Significance Microglia are the resident phagocytic cells of central nervous system (CNS). During demyelination, a condition in which myelin sheaths damaged and removed from neurons due to inflammation, microglia can play both harmful beneficial roles, but their exact functions still unclear. Using drug that depletes microglia, we demonstrate that, after infection with JHMV, coronavirus infects CNS rodents causes immune-mediated essential for repair following demyelination. We found played...
The ongoing coronavirus (CoV) disease 2019 (COVID-19) pandemic caused by infection with severe acute respiratory syndrome CoV 2 (SARS-CoV-2) is associated substantial morbidity and mortality. Understanding the immunological pathological processes of diseases crucial for rational design effective vaccines therapies COVID-19. Previous studies showed that 2'-O-methylation viral RNA cap structure required to prevent recognition RNAs intracellular innate sensors. Here, we demonstrate guanine...
Toll-like receptor-3 (TLR-3) recognizes double-stranded RNA and induces multiple intracellular events responsible for innate anti-viral immunity against a number of viral infections. Activation TLR-3 inhibits human immunodeficiency virus (HIV) replication, but the mechanism(s) underlying action activation on HIV are largely unknown. Here we demonstrate that treatment monocyte-derived macrophages with poly I:C, synthetic ligand TLR-3, significantly inhibited infection replication....
(−)-Epigallocatechin gallate (EGCG) is a major polyphenol component of green tea that has antioxidant activities. Lipopolysaccharide (LPS) induces inflammatory cytokine production and impairs blood–brain barrier (BBB) integrity. We examined the effect EGCG on LPS-induced expression cytokines in human cerebral microvascular endothelial cells (hCMECs) BBB permeability. The TNF-α, IL-1β monocyte chemotactic protein-1 (MCP-1/CCL2) was determined by quantitative real time PCR (qRT-PCR) ELISA....
Accumulative studies suggest that inflammatory bowel disease (IBD) may cause multiple central nervous system (CNS) pathologies. Studies have found indoleamine-2,3-dioxygenase (IDO, rate-limiting enzyme of the kynurenine (Kyn) pathway) deficient mice were protected from endotoxin induced cognitive impairment, and Kyn administration memory deficits in both control IDO-deficient mice. However, there is no investigation brain pathway IBD, thus we investigated whether dextran sulfate sodium...
The classification of microglial M1/M2 polarization in the acute phase ischemic stroke remains controversial, which has limited further advances neuroprotective strategy. To thoroughly assess phenotypes, we made middle cerebral artery occlusion model mice to simulate pathological processes from normal conditions ischemia and then early reperfusion period. temporal changes gene profiles, cell subtypes, function were comprehensively analyzed using single-cell RNA sequencing. We identified...
The immune systems can be altered by spaceflight in many aspects, but microgravity-related mucosal changes and its clinical significance have not been well studied. purpose of this study was to investigate whether simulated microgravity influences the intestinal homeostasis increases susceptibility colon inflammation. hindlimb unloading (HU) mouse model used simulate condition. Three percent dextran sulfate sodium (DSS) given mice induce colitis. Compared ground control (Ctrl) mice, HU ones...
Significance Both genetic and environmental factors, especially viruses, may contribute to the development of multiple sclerosis. In this study, we find that oligodendrocytes (OLs) survive infection with a neurotropic coronavirus, mouse hepatitis virus, for at least 150 d. These surviving OLs are located adjacent demyelinating regions express genes chronic inflammatory state. Notably, extent cell infiltration subsequent ability regain normal OL morphology varied depending upon anatomic...
The environmental conditions that could lead to an increased risk for the development of infection during prolonged space flight include: microgravity, stress, radiation, disturbance circadian rhythms, and altered nutritional intake. A large body literature exists on impairment immune system by flight. With advent missions outside Earth's magnetic field, adverse effects due exposure radiation from a solar particle event (SPE) needs be considered. Using models reduced gravity SPE we identify...
A major risk of extended space travel is the combined effects weightlessness and radiation exposure on immune system. In this study, we used hindlimb suspension model microgravity that includes other stressors, situational confinement stress alterations in food intake, solar particle event (SPE)-like to measure ability control bacterial infections. massive increase morbidity decrease growth was observed using 2 different types bacteria delivered by systemic pulmonary routes 3 strains mice....
Abstract Purpose Early detection of ocular abnormalities in newborns is essential for timely diagnosis and treatment. This study aimed to assess the 1‐year result a multicentre prospective neonatal eye examination programme with wide‐field digital imaging system China. Methods A collaborative group screening was established nine hospitals, including eight Maternal Children's Hospitals, one general hospital across China from July 2016 June 2017. Ocular examinations were performed on within 28...
Recent reports indicate that neural stem cells (NSCs) exist in a cluster-like formation close proximity to cerebral microvessels. Similar appearing clusters can be seen ex vivo NSC cultures termed neurospheres. It is known this neurosphere configuration important for preserving stemness and proliferative state. How NSCs form neurospheres or neuroclusters remains largely undetermined. In study, we show primary human express the tight junction proteins (TJPs): zonula occludens-1 (ZO-1),...
Space flight conditions within the protection of Earth's gravitational field have been shown to alter immune responses, which could lead potentially detrimental pathology. An additional risk extended space travel outside is effect solar particle event (SPE) radiation exposure on system. Organisms that infection include endogenous, latent viruses, colonizing pathogenics, and commensals, as well exogenous microbes present in spacecraft or other astronauts. In this report, SPE-like containment...
Toll-like receptor 3 (TLR3) activation plays an important role in the innate immune responses to viral infections. We show here that of TLR3 signaling pathway by poly I:C, a synthetic mimic dsRNA, could induce high-level expression interferon (IFN)- λ1 hepatoma cell line. The induced IFN-λ1 contributed I:C-mediated inhibition hepatitis C virus (HCV) Japanese fulminant hepatitis-1 (JFH-1) replication Huh7 cells. This inhibitory effect I:C on HCV replication, however, was compromised infection...
Summary There is limited information about the role of hepatic stellate cells ( HSC ) in liver innate immunity against hepatitis C virus HCV ). We thus examined whether can produce antiviral factors that inhibit replication human hepatocytes. expressed functional T oll‐like receptor 3 TLR ‐3), which could be activated by its ligand, polyinosine‐polycytidylic acid (poly I : ), leading to induction interferon‐λ IFN ‐λ) at both mRNA and protein levels. ‐3 signalling also induced expression...
The ongoing coronavirus disease-2019 (COVID-19) pandemic caused by severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) has serious influences on human health and economy. available clinical data suggest that patients infected SARS-CoV-2 have the possibility of simultaneous infection bacteria. In this study, we present a data-driven mathematical model for coinfection bacteria to investigate dynamics COVID-19 progress. Specifically, based statistical analysis different from China some...
Diabetes increases the occurrence and severity of atherosclerosis. When plaques form in brain vessels, cerebral atherosclerosis causes thickness, rigidity, unstableness artery walls, leading to severe complications like stroke contributing cognitive impairment. So far, molecular mechanism underlying is not determined. Moreover, effective intervention strategies are lacking. In this study, we showed that polarization microglia, resident macrophage central nervous system, appeared play a...
Macrophages play critical roles in innate immune response the liver. Whether macrophages participate liver immunity against HCV replication is poorly understood.The aim of this study was to investigate role replication.Freshly isolated monocytes were purified from peripheral blood healthy adult donors. refer 7-day-cultured vitro. A hepatoma cell line (Huh7) infected with JFH-1 generate vitro infectious system. RT-PCR used determine RNA and mRNA levels genes expression. ELISA measure protein...