A5100322664- Cholesterol and Lipid Metabolism
- Lipoproteins and Cardiovascular Health
- Peroxisome Proliferator-Activated Receptors
- Lipid metabolism and disorders
- Receptor Mechanisms and Signaling
- Diabetes Treatment and Management
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Atherosclerosis and Cardiovascular Diseases
- Drug Transport and Resistance Mechanisms
- Adipose Tissue and Metabolism
- Heat shock proteins research
- Phagocytosis and Immune Regulation
- Cancer, Lipids, and Metabolism
- Helicobacter pylori-related gastroenterology studies
- Tissue Engineering and Regenerative Medicine
- Diet, Metabolism, and Disease
- Endoplasmic Reticulum Stress and Disease
- CRISPR and Genetic Engineering
- Cancer-related molecular mechanisms research
- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Natural Antidiabetic Agents Studies
- Curcumin's Biomedical Applications
- Immune Response and Inflammation
- Protein Hydrolysis and Bioactive Peptides
Wuhan University
2016-2025
Peking University
2023-2024
Zhongda Hospital Southeast University
2024
Sichuan Agricultural University
2024
Ministry of Agriculture and Rural Affairs
2024
EarthTech International (United States)
2024
Peking University Third Hospital
2023
Shihezi University
2023
Huazhong University of Science and Technology
2022
Tongji Hospital
2022
Proprotein convertase subtilisin/kexin type 9 (PCSK9) regulates serum LDL cholesterol (LDL-C) by interacting with the receptor (LDLR) and is an attractive therapeutic target for LDL-C lowering. We have generated a neutralizing anti-PCSK9 antibody, mAb1, that binds to epitope on PCSK9 adjacent region required LDLR interaction. In vitro, mAb1 inhibits binding attenuates PCSK9-mediated reduction in protein levels, thereby increasing uptake. A combination of statin increases levels HepG2 cells...
Stem cell implantation strategy has exhibited potential to treat the myocardial infarction (MI), however, low retention and survival limit their applications due reactive oxygen species (ROS) microenvironment after MI. In this study, fullerenol nanoparticles are introduced into alginate hydrogel create an injectable delivery vehicle with antioxidant activity. Results suggest that prepared hydrogels exhibit excellent mechanical strength. addition, fullerenol/alginate can effectively scavenge...
A missing link in cholesterol absorption Cholesterol is important for general health, but too much can build up artery walls and cause cardiovascular disease. Low-density lipoprotein (LDL-C) often referred to as “bad cholesterol”; keeping LDL-C within stringent limits recommended reduce the risk of heart attack stroke. Zhang et al. discovered that some individuals have an inherited frameshift mutation LIMA1 gene (also known EPLIN or SREBP3 ). The has not been linked lipid metabolism before,...
High blood cholesterol accelerates the progression of atherosclerosis, which is an asymptomatic process lasting for decades. Rupture atherosclerotic plaques induces thrombosis, results in myocardial infarction or stroke. Lowering levels beneficial preventing cardiovascular disease. Low-density lipoprotein (LDL) receptor (LDLR) was used as bait to identify its binding proteins plasma, and coagulation factor prekallikrein (PK; encoded by KLKB1 gene) revealed. The correlation between serum PK...
BACKGROUND: Low-density lipoprotein (LDL) is internalized into cells mainly through LDLR (LDL receptor)–mediated endocytosis. In an acidic endosome, released from LDL and recycles back to the cell surface, whereas left in endosome degraded lysosome. Circulating PCSK9 (proprotein convertase subtilisin/kexin 9) binds with similar LDL. fails disassociate PCSK9, both proteins are inhibitors widely used for treating hypercholesterolemia. However, how diverts lysosome degradation remains elusive....
Abstract Background AsCas12a and LbCas12a nucleases are reported to be promising tools for genome engineering with protospacer adjacent motif (PAM) TTTV as the optimal. However, C-containing PAM (CTTV, TCTV, TTCV, etc.) recognition by Cas12a might induce extra off-target edits at these non-canonical sites. Results Here, we identify a novel nuclease CeCas12a from Coprococcus eutactus , which is programmable genome-editing efficiencies comparable in human cells. Moreover, revealed more...
The transport of LDL-derived cholesterol from lysosomes to peroxisomes is facilitated by membrane contacts formed between the lysosomal protein synaptotagmin VII and peroxisomal lipid phosphatidylinositol 4, 5-bisphosphate [PI(4,5)P2]. Here, we used RNA interference search for regulators PI(4,5)P2 study effects altered homeostasis on transport. We found that knockdown 5-phosphate 4-kinase type-2 α (PIP4K2A) reduced levels, decreased lysosome-peroxisome contacts, increased accumulation in...
Lower plasma levels of LDL (low-density lipoprotein) cholesterol (LDL-C) can reduce the risk atherosclerotic cardiovascular disease. The loss-of-function mutations in PCSK9 (proprotein convertase subtilisin/kexin type 9) have been known to associate with low LDL-C many human populations. genetic variants Chinese Uyghurs who are at high disease due their dietary habits not reported. study involved whole-exome and target sequencing college students from Uyghur other ethnic groups Xinjiang,...
Compared with sulfide tailings, the oxidation and transformation of certain substances in oxidized tailings into more soluble forms may affect bioaccumulation biomagnification properties enhance risk toxic effects ecosystem. This study aimed to apply generalized additive model (GAM) evaluate factors affecting heavy metal leaching from nickel (Ni) tailings. We created an orthogonal experiment table (L18(37)) evenly distribute different combinations factor values. The Ni were immersed...
Sarcopenia is a chronic, progressive disease characterized by the gradual loss of skeletal muscle strength and mass. This study investigates role long non-coding RNA GPRC5D-AS1 in development progression sarcopenia through its regulation SLC7A11 . Skeletal samples were obtained from patients healthy controls to assess expression levels Flow cytometry was used evaluate iron content, lipid peroxidation, antioxidant markers. A ferroptosis model established human cells (HSKM) using inducer...
In the process of membrane biogenesis several dozen proteins must operate in precise concert to generate ≈100 lipids at appropriate concentrations. To study regulation bilayer assembly a cell cycle-independent manner, we have exploited fact that phagocytes replenish membranes expended during particle engulfment rapid phase lipid synthesis. response phagocytosis latex beads, human embryonic kidney 293 cells synthesized cholesterol and phospholipids amounts equivalent surface area internalized...
Familial hypercholesterolemia (FH) is a common monogenic disease, while studies about the epidemiology for general population in China was scarce. Aim of study to estimate prevalence FH and explore related factors by Chinese modified Dutch Lipid Clinic Network (DLCN) definition. A total 39,205 participants (15,463 males 23,742 females) aged 18–79 years old were enrolled from Henan Rural Cohort Study. defined DLCN definition, score > 5 classified as FH. Logistic regression analysis used...