A5069715775- HIV Research and Treatment
- Immune Cell Function and Interaction
- Hepatitis C virus research
- Neuropeptides and Animal Physiology
- interferon and immune responses
- Cytomegalovirus and herpesvirus research
- Receptor Mechanisms and Signaling
- HIV/AIDS Research and Interventions
- Hepatitis B Virus Studies
- Herpesvirus Infections and Treatments
- Immune Response and Inflammation
- Neuroinflammation and Neurodegeneration Mechanisms
- Liver Disease Diagnosis and Treatment
- T-cell and B-cell Immunology
- HIV-related health complications and treatments
- SARS-CoV-2 and COVID-19 Research
- HIV/AIDS drug development and treatment
- Tryptophan and brain disorders
- Animal Virus Infections Studies
- Reproductive System and Pregnancy
- Chemokine receptors and signaling
- COVID-19 Clinical Research Studies
- Pain Mechanisms and Treatments
- Tea Polyphenols and Effects
- Immune cells in cancer
Temple University
2014-2024
Temple College
2020-2021
Wuhan University
2006-2017
Creative Research Enterprises (United States)
2017
State Key Laboratory of Virology
2011-2013
Children's Hospital of Philadelphia
2000-2009
University of Pennsylvania
2000-2009
Renmin Hospital of Wuhan University
2006-2008
Philadelphia University
1994-2006
Foundation for the National Institutes of Health
2004
Abstract We present data demonstrating the gene expression of substance P and its receptor in human peripheral blood-isolated monocytes macrophages. Using RT-PCR assay, preprotachykinin-A (substance P) mRNA is detected Among alpha, beta, gamma transcripts gene, only beta are detectable these cells. By Southern blot assay RT-PCR-amplified recognized using a specific probe. Sequence analysis products from both macrophages also confirmed structure transcripts, which identical to those found...
We present data demonstrating the gene expression of substance P (SP) and its receptor in human peripheral blood-isolated lymphocytes. Using reverse transcribed polymerase chain reaction (RT-PCR) assay, preprotachykinin-A (substance-P) mRNA is detected Among α, β γ transcripts SP gene, only are detectable these cells. These RT-PCR amplified recognized by Southern blot assay using a specific probe. Direct DNA sequence analysis products from lymphocytes also confirmed structure which identical...
The newly identified type III interferon (IFN-lambda) has antiviral activity against a broad spectrum of viruses. We thus examined whether IFN-lambda the ability to inhibit human immunodeficiency virus 1 (HIV-1) infection blood monocyte-derived macrophages that expressed receptors. Both IFN-lambda1 and IFN-lambda2, when added macrophage cultures, inhibited HIV-1 replication. This IFN-lambda-mediated anti-HIV-1 is broad, as could by both laboratory-adapted clinical strains HIV-1....
As the COVID-19 pandemic rages on, new SARS-CoV-2 variants have emerged in different regions of world. These newly mutations their spike (S) protein that may confer resistance to vaccine-elicited immunity and existing neutralizing antibody therapeutics. Therefore, there is still an urgent need safe, effective, affordable agents for prevention/treatment its variant infection.We demonstrated green tea beverage (GTB) or major ingredient, epigallocatechin gallate (EGCG), were highly effective...
Abstract CB2 expression is upregulated in HIV-1 infected macrophages; agonists limits infection by inhibition of the HIV-1-LTR. Infiltrating monocytes and macrophages play a crucial role progression CNS. Previous studies showed that activation can attenuate inflammatory responses affect infectivity T cells microglia. Here, we report also act as immunomodulators on HIV-1-infected macrophages. First, our findings indicated presence elevated levels monocytes/macrophages perivascular cuffs...
Opiate abuse has been postulated to be a cofactor in the immunopathogenesis of acquired immunodeficiency syndrome (AIDS). This study evaluated whether methadone, drug widely prescribed for treatment abusers with opioid dependence, affects human virus (HIV) infection immune cells. When added fetal microglia and blood monocyte–derived macrophage cultures, methadone significantly enhanced HIV these enhancement was associated up-regulation expression CCR5, primary coreceptor macrophage-tropic...
Biological interactions between human cytomegalovirus (HCMV) and the immunodeficiency virus type 1 (HIV-1) were analysed in transfection infection experiments, carried out a osteogenic sarcoma cell line (HOS) same chronically infected with HCMV (E155). When HOS El55 cells transfected recombinant plasmids containing HIV long terminal repeat (LTR) linked to bacterial chloramphenicol acetyl- transferase (CAT) gene, LTR-directed CAT expression was 20 times higher than cells. co-infected HIV-1...
J. Neurochem. (2011) 118 , 1113–1123. Abstract Excitotoxic neuronal damage via over‐activation of the NMDA receptor has been implicated in many neurodegenerative diseases. In vitro modeling excitotoxic injury shown that activation G‐protein coupled receptors (GPCRs) counteracts such through modulation pro‐survival pathways and/or signaling. We have previously demonstrated GPCR APJ and its endogenous neuropeptide ligand apelin can protect neurons against excitotoxicity, but mechanism(s) this...
Microglia are the primary target and reservoir of HIV infection in central nervous system (CNS) which contribute to HIV-associated neurocognitive disorder (HAND). However, studying microglia has been challenged by limited availability human microglial cells. To overcome this issue, investigators have developed various models for studies, including immortalized cell lines, latently infected clone, peripheral blood monocyte-derived (MMG), induced pluripotent stem (iPSC)-derived (iMg)...
Opioid use disorders (OUD) exacerbate the complexity of neurocognitive impairments and neurodevelopmental disorders, with poorly understood molecular mechanisms at gene regulatory level. This study utilized single-nuclei RNA sequencing (snRNA-seq) data from SCORCH (Single-Cell Responses in Context HIV) program, examining brain samples 95 donors, including individuals without a history opioid abuse, to explore networks within ventral tegmental area (VTA). While traditional differential...
Opioid use disorders (OUD) exacerbate the complexity of neurocognitive impairments and neurodevelopmental disorders, with poorly understood molecular mechanisms at intercellular communication level. This study utilized single-nuclei RNA sequencing (snRNA-seq) data from SCORCH program, examining brain samples 95 donors, including individuals without a history opioid abuse, to explore cellular networks within ventral tegmental area (VTA). Our analysis, using CellChat quantify interactions...
Abstract Elevated brain lactate has been observed by in vivo proton MRS different pathological situations. The origin of this remains controversial. possibility that it was produced the metabolism phagocytic cells proposed. To investigate hypothesis, authors have employed high‐resolution to monitor changes glucose, lactate, and other metabolites medium used culture human monocyte‐derived macrophages vitro. Results show differentiation monocytes/macrophages presence physiological stimulating...
Glutathione (GSH), its derivatives and N-acetylcysteine (NAC) inhibit the induction of HIV-1 expression in a chronically HIV-1-infected promonocytic cell line (Ul/HIV) peripheral blood mononuclear cells (PBMC). We have examined effects GSH NAC on replication human primary monocyte/macrophages cultured vitro. Ficoll-gradient purified monocytes were cultivated vitro for 7-10 days then infected with (Bal Ada-M). Infection was blocked or substantially reduced by (5-20 mM). Significant reduction...
Substance P (SP) is a potent modulator of neuroimmunoregulation. We recently reported that human immune cells express SP and its receptor. have now investigated the possible role receptor plays in HIV infection mononuclear phagocytes. enhanced replication blood-isolated phagocytes, whereas nonpeptide antagonist (CP-96,345) potently inhibited infectivity these concentration-dependent fashion. CP-96,345 prevented formation typical giant syncytia induced by Bal strain cells. This inhibitory...
Substance P (SP) is an undecapeptide that has the amino sequence Arg-Pro-Lys-Pro-Gin-Gln-Phe-Phe-Gly-Leu-Met-NH2 and belongs to a family of structurally related peptides known as tachykinins, latter are widely distributed in central nervous system. SP involved biological activities cells immune system, including induction cytokines cells. We have investigated effects on constitutive and/or lipopolysaccharide (LPS)-induced expression tumor necrosis factor (TNF) cultured blood monocyte-derived...
Abstract The chronicity of hepatitis C virus (HCV) infection raises the question how HCV is able to persist in hepatic cells. We show that human primary hepatocytes and cell lines (Huh7 HepG2) spontaneously produce interferon (IFN)-α inhibited replicon cells (Huh.8 FCA-1). Silencing IFN-α gene expression by small interfering RNA (siRNA) resulted increased expression. activation regulatory factor (IRF-7) led inhibition expression, whereas anti–IFN-α receptor antibody could partially block...