A5054729027- RNA modifications and cancer
- DNA Repair Mechanisms
- Cancer-related molecular mechanisms research
- Heme Oxygenase-1 and Carbon Monoxide
- RNA Research and Splicing
- Neonatal Health and Biochemistry
- Redox biology and oxidative stress
- PARP inhibition in cancer therapy
- Genomics, phytochemicals, and oxidative stress
- Ovarian cancer diagnosis and treatment
- Sulfur Compounds in Biology
- Organ Transplantation Techniques and Outcomes
- DNA and Nucleic Acid Chemistry
- Hemoglobin structure and function
- Steroid Chemistry and Biochemistry
- Soft Robotics and Applications
- Adenosine and Purinergic Signaling
- Thyroid Cancer Diagnosis and Treatment
- Advanced biosensing and bioanalysis techniques
- Galectins and Cancer Biology
- PI3K/AKT/mTOR signaling in cancer
- Trace Elements in Health
- Endoplasmic Reticulum Stress and Disease
- Cell Adhesion Molecules Research
- Amyotrophic Lateral Sclerosis Research
Centro di Riferimento Oncologico
2016-2023
Istituti di Ricovero e Cura a Carattere Scientifico
2018-2023
University of Udine
2004-2015
Fondazione Italiana Fegato
2013
University of Naples Federico II
2010
University of Trieste
2002-2005
APE1/Ref-1 (hereafter, APE1), a DNA repair enzyme and transcriptional coactivator, is vital protein in mammals. Its role controlling cell growth the molecular mechanisms that fine-tune its different cellular functions are still not known. By an unbiased proteomic approach, we have identified characterized several novel APE1 partners which, unexpectedly, include number of proteins involved ribosome biogenesis RNA processing. In particular, interaction between nucleophosmin (NPM1) was...
Oxidative stress is a major pathogenetic event occurring in several liver disorders ranging from metabolic to proliferative ones, and cause of damage due Ischemia/Reperfusion (I/R) during transplantation. The main sources ROS are represented by mitochondria cytocrome P450 enzymes the hepatocyte, Kupffer cells neutrophils. Cells provided with efficient molecular strategies strictly control intracellular level maintain balance between oxidant antioxidant molecules. A cellular oxidative...
Apurinic/apyrimidinic endonuclease 1 (APE1), an essential protein in mammals, is involved base excision DNA repair (BER) and regulation of gene expression, acting as a redox co-activator several transcription factors. Recent findings highlight novel role for APE1 RNA metabolism, which modulated by nucleophosmin (NPM1). The results reported this article show that five lysine residues (K24, K25, K27, K31 K32), located the N-terminal unstructured domain, are interaction with both NPM1, thus...
Abstract Apurinic apyrimidinic endonuclease/redox effector factor 1 (APE1/Ref‐1) protects cells from oxidative stress by acting as a central enzyme in base excision repair pathways of DNA lesions and through its independent activity redox transcriptional co‐activator. Dysregulation this protein has been associated with cancer development. At present, contrasting data have published regarding the biological relevance two functions well molecular mechanisms involved. Here, we combined both...
APE1/Ref-1, normally localized in the nucleus, is a regulator of cellular response to oxidative stress. Cytoplasmic localization has been observed several tumors and correlates with poor prognosis. Because no data are available on liver tumors, we investigated APE1/Ref-1 subcellular its correlation survival 47 consecutive patients undergoing hepatocellular carcinoma (HCC) resection. expression was determined by immunohistochemistry HCC surrounding cirrhosis (SLC) compared normal tissue....
Inhibitors of histone deacetylases (HDACs) activate the sodium iodide symporter (NIS) expression in thyroid tumor cells. In this study, mechanisms accounting for these effects were investigated. Various human cell lines (ARO, BCPAP, FRO, TPC-1) treated with HDAC inhibitors Na butyrate (NaB) and tricostatin A (TSA), on NIS several thyroid-specific transcription factors together activity promoter evaluated. TSA NaB increased mRNA levels all lines. Among factors, only PAX8 ARO cells was...
Oxidative stress is a major pathogenetic event occurring in several liver disorders and cause of damage due to Ischemia/Reperfusion (I/R) during transplantation. While markers chronic oxidative are well known, early protein targets injury not defined. In order identify these proteins, we used differential proteomics approach HepG2 human cells treated for 10 min with 500 microM H(2)O(2). This dose was sufficient induce slight decrease total GSH thiol content without affecting cell viability....
Knowledge of early molecular events occurring upon ischemia/reperfusion (I/R) during liver transplantation (LT) is great importance to improve the therapeutic intervention surgical treatment. However, nowadays, few data are available on protein targets I/R injury. To identify these proteins, we used a differential proteomics approach in characterization human biopsies LT. Analyses were performed nine donor livers By using 2-DE and MALDI-TOF MS, identified 36 proteins which resulted...
APE1/Ref-1 is a main regulator of cellular response to oxidative stress via DNA-repair function and co-activating activity on the NF-κB transcription factor. APE1 central in controlling stress-based inflammatory processes through modulation cytokines expression its overexpression responsible for onset chemoresistance different tumors including hepatic cancer. We examined functional role during cell damage related fatty acid accumulation redox process. HepG2 cells were stably transfected with...
Abstract Severe hyperbilirubinemia is toxic during central nervous system development. Prolonged and uncontrolled high levels of unconjugated bilirubin lead to bilirubin-induced encephalopathy eventually death by kernicterus. Despite extensive studies, the molecular cellular mechanisms toxicity are still poorly defined. To fill this gap, we investigated processes underlying neuronal injury in a mouse model severe neonatal jaundice, which develops as consequence null mutation Ugt1 gene. These...
Abstract Unconjugated bilirubin (UCB) is a powerful antioxidant and modulator of cell growth through the interaction with several signal transduction pathways. Although newborns develop physiological jaundice, in case severe hyperbilirubinemia UCB may become neurotoxic causing long‐term neuronal damages, also known as encephalopathy. To investigate mechanisms UCB‐induced toxicity, we used human neuroblastoma line SH‐SY5Y an vitro model system. We verified that caused death, part due to...
Abstract Rs3814113 is the single-nucleotide polymorphism (SNP) showing strongest association with high-grade serous ovarian carcinoma (HGSOC) incidence and located in an intergenic region about 44 kb downstream of basonuclin 2 ( BNC2 ) gene. Lifetime number ovulations associated increased risk to develop HGSOC, probably because cell damage extrauterine Müllerian epithelium by ovulation-induced oxidative stress. However, impact low-penetrance HGSOC alleles (e.g. rs3814113) on induced stress...
Extracellular nucleotides exert an important role in controlling cell physiology by activating intracellular signalling cascades. Osteoblast HOBIT cells express P2Y1 and P2Y2 G-protein-coupled receptors, respond to extracellular ATP increasing cytosolic calcium concentrations. Early growth response protein 1 (Egr-1) is a C2H2-zinc-finger-containing transcriptional regulator responsible for the activation of several genes involved control proliferation apoptosis, thought have central...
The lipid phosphatase, phosphatase and tensin homolog (PTEN), is a key element in controlling cell growth survival has well established role as tumor suppressor protein many neoplasia. Several data indicate that silencing of PTEN gene expression may be relevant follicular thyroid transformation. Thus, the present study regulation cells was investigated. Cotransfection experiments indicated normal FRTL-5 rat cells, promoter activity increased by overexpression transcription factor early...
APE1/Ref-1 is a main regulator of cellular response to oxidative stress via DNA-repair function and co-activating activity on the NF-kB transcription factor.APE1 central in controlling stress-based inflammatory processes through modulation cytokines expression its overexpression responsible for onset chemoresistance different tumors including hepatic cancer.We examined functional role APE1 during cell damage related fatty acid accumulation redox process.HepG2 cells were stably transfected...
The identification of reliable markers for diagnosis breast cancer has been thoroughly addressed by metabolic profiling using nuclear magnetic resonance (NMR) spectroscopy or imaging. Several clear diagnostic indicators have emerged either in vitro analysis tissue extracts, ex vivo biopsies direct spectral observations. Most the characteristic metabolites could be assayed mass spectrometry (MS) to exploit superior sensitivity this technique and therefore reduce traumatic impact current...
BackgroundMetastatic colorectal cancer (CRC) remains a deadly disease. Identifying locally advanced CRC patients with high risk of developing metastasis and improving outcome metastatic require discovering master regulators metastasis. In this context, the non-coding part human genome is still largely unexplored.MethodsTo interrogate disclose metastasis, we combined transposon-based forward genetic screen novel in vitro assay, which forces cells to grow deprived cell-substrate cell-cell...
TP53 is the most frequently mutated gene in human cancers. Most genomic alterations are missense mutations, which cause a loss of its tumour suppressor functions while providing mutant p53 (mut_p53) with oncogenic features (gain-of-function). Loss alters transcription both protein-coding and non-protein-coding genes. Gain-of-function mut_p53 triggers modification expression as well; however, impact on genes whether these affect properties cancer cell lines not fully explored. In this study,...