Abstract Background Patients with lymph node (LN)-positive pancreatic ductal adenocarcinoma (PDAC) have extremely poor survival rates. Circular RNAs (circRNAs), a newly discovered type of endogenous noncoding RNAs, been proposed to mediate the progression diverse types tumors. However, role and underlying regulatory mechanisms circRNAs in LN metastasis PDAC remain unknown. Methods Next-generation sequencing was used identify differentially expressed between normal adjacent tissues. In vitro...

Estrogen-dependent breast cancer is often treated with the aromatase inhibitors or estrogen receptor (ER) antagonists. Tamoxifen as a major ER antagonist usually used to treat those patients ERα-positive cancer. However, majority of ERα positive fail respond tamoxifen due presence intrinsic acquired resistance drug. Altered expression and functions microRNAs (miRNAs) have been reportedly associated resistance. In this study, we investigated role miR-27b-3p in tamoxifen. MiR-27b-3p levels...

Lymphatic metastasis constitutes a leading cause of recurrence and mortality in bladder cancer. Accumulating evidence indicates that lymphangiogenesis is indispensable to trigger lymphatic metastasis. However, the specific mechanism poorly understood. In present study, we revealed pathway involved cancer, which circular RNA (circRNA) facilitated vascular endothelial growth factor C (VEGF-C)-independent manner. Novel circRNA circEHBP1 was markedly upregulated cancer correlated positively with...

It is well known that local anesthetics have a broad spectrum of pharmacological actions, acting as nerve blocks, and treating pain cardiac arrhythmias via blocking the sodium channel. The use could reduce possibility cancer metastasis recurrence following surgical tumor excision. purpose present study was to investigate inhibitory effect lidocaine upon invasion migration transient receptor potential cation channel subfamily V member 6 (TRPV6)-expressing cells. Human breast MDA-MB-231 cells,...

Deoxynivalenol (DON) pollution is prevalent in crops, and can induce oxidative stress intestinal injury. Hesperidin one of the major flavonoids citrus fruits that has various biological activities such as antioxidant anti-inflammatory activities. However, whether hesperidin could alleviate DON-induced injury mechanism remain unclear. Mitochondria-associated endoplasmic reticulum (ER) membranes (MAMs) have attracted attention for their crucial signaling points to regulate ER-mitochondria...

Conventional smear cytology (CSC) is a specific method used for breast tumor diagnosis in patients with nipple discharge. However, CSC tends to miss diagnose or even misdiagnose due contaminating blood cells and other impurities. Thus, it critical develop more accurate sensitive methods clinical evaluation. Using discharge collected directly from without aspiration, we performed liquid-based (LBC) analyze 111 samples cytological evaluation the first time. Following centrifugation remove...

This study aimed to assessed whether Talin-1 is involved in the pathogenesis of aortic dissection via regulating vascular smooth muscle cell (VSMC) biological function. Human samples were obtained from organ donors who died nonvascular diseases as normal controls and patients undergoing surgical repair thoracic dissection. The expression level distribution detected using westernblot analysis immunohistochemistry each sample. We inhibited RNA interference VSMCs. VSMC proliferation was by...

Rationale: Aortic dissection (AD) is caused by functional disorder of cells in the aortic wall, which largely attributed to vascular remodeling.Therapeutic strategies for AD remain limited due our incomplete understanding role endothelial (ECs) pathogenesis.This study aimed identify regulatory miR-27a and provide a mechanistic basis non-invasive treatment AD.Methods: We harvested aortas from normal patients explore expression miR-27a.In vitro vivo assays were preformed biological effects...

Abstract The insulin-like growth factor 1 receptor (IGF-1R) governs several signaling pathways for cell proliferation, survival, and anti-apoptosis. Thus, targeting IGF-1R appears as a reasonable rationale tumor treatment. However, clinical studies showed that inhibition of has very limited efficacy due to the development resistance blockade in cells. Here, we discovered prolonged treatment colon cancer cells with inhibitors (BMS-754807 GSK1838705A) stimulates p70 KDa ribosomal protein S6...

The testin (TES) gene was previously identified in the fragile chromosomal region FRA7G at 7q31.2. In present study, we aimed to investigate candidate tumor suppressor function of TES and explore its correlations clinicopathologic features prognosis breast cancer. clinical samples, showed that expression decreased gradually from normal through ductal hyperplasia without atypia, atypical hyperplasia, carcinoma situ, invasive carcinoma. To possible suppressing TES, cancer cells manipulated by...

Abstract Autophagy and ER stress participated in the inhibition of MPP a‐ PDT on tumor growth, but molecular links between them remain undefined. We just explore mechanism vitro vivo. CCK ‐8 assay flow cytometer were used to detect cytotoxicity mode cell death after . Furthermore, role autophagy was verified Confocal microscopy show intracellular distribution a. markers PERK signaling pathway detected by western blot. While vivo, histology immunohistochemistry performed effect mice. After ,...

Despite recent evidence, the role of human papillomavirus (HPV) in breast carcinogenesis is controversial. The correlations HPV infection with clinicopathological features cancer and expression cell cycle/apoptosis-associated proteins have not been well elucidated. In this study, we sought to determine prevalence high-risk HPVs (HR-HPVs) BCL2, p21, p53, Rb, survivin patients investigate relationship these cancer-related proteins, an attempt clarify potential mechanism pathogenesis. presence...

The aim of this study was to investigate the relationship between Talin-1 and stability carotid atherosclerosis plaque also find out role miRNA, as an upstream regulator, in regulating expression level Talin-1.Human plaques were obtained from 20 symptomatic stenosis patients who underwent endarterectomy (CEA) our hospital October 2014 August 2017. Western blot analysis immunohistochemistry carried detect distribution each sample. content miRNAs decected by quantitative reverse transcription...

Abstract Background Circular RNAs (circRNAs) have been shown to participate in the chemoresistance and tumorigenesis of multiple cancers. The purpose this research was investigate function circ_0081001 methotrexate (MTX) resistance osteosarcoma (OS) its potential molecular mechanism. Methods expression circ_0081001, cytochrome P450 family 51 subfamily A member 1 (CYP51A1), miR-494-3p detected by qRT-PCR. Cell viability, apoptosis, migration, invasion were evaluated Counting Kit-8 (CCK-8)...

As primary flavonoids extracted from citrus fruits, hesperidin has been attracting attention widely for its capacity to act as antioxidants that are able scavenge free radicals and reactive oxygen species (ROS). Many factors have made oxidative stress a risk factor the occurrence of intestinal barrier injury, which is serious health threat human beings. However, little data available regarding underlying mechanism alleviating injury under stress. Recently, endoplasmic reticulum (ER)...

Abstract MiR‐4732‐5p was previously found to be dysregulated in nipple discharge of breast cancer. However, the expression and function miR‐4732‐5p cancer remain largely unknown. Here, detected using quantitative real‐time PCR tissues cell lines. Cell proliferation, apoptosis, migration invasion assays were performed examine effects In addition, mRNA sequencing, bioinformatics analysis, Western blot luciferase identify target miR‐4732‐5p. Overall, significantly down‐regulated tissues,...

Abstract Spinal Cord Injury (SCI) is a condition characterized by complete or incomplete motor and sensory impairment, as well dysfunction of the autonomic nervous system, caused factors such trauma, tumors, inflammation. Current treatment methods primarily include traditional approaches like spinal canal decompression internal fixation surgery, steroid pulse therapy, newer techniques stem cell transplantation brain-spinal cord interfaces. However, above have limited efficacy in promoting...