A5018671046- Gut microbiota and health
- Coronary Interventions and Diagnostics
- Ion Channels and Receptors
- Diet and metabolism studies
- Liver Disease Diagnosis and Treatment
- Insect Pest Control Strategies
- Neurobiology and Insect Physiology Research
- ATP Synthase and ATPases Research
- Mitochondrial Function and Pathology
- Drug-Induced Hepatotoxicity and Protection
- Insect and Pesticide Research
- Pancreatic function and diabetes
- Diet, Metabolism, and Disease
- Pesticide Exposure and Toxicity
- Physiological and biochemical adaptations
- Calcium signaling and nucleotide metabolism
- Multiple Myeloma Research and Treatments
- Cancer, Stress, Anesthesia, and Immune Response
- Pancreatitis Pathology and Treatment
- Chemokine receptors and signaling
- Bone Tissue Engineering Materials
- Piperaceae Chemical and Biological Studies
- Dietary Effects on Health
- Ion channel regulation and function
- Cardiac and Coronary Surgery Techniques
University of California, San Francisco
2024
Southern Medical University
2018-2023
Zhujiang Hospital
2023
Nankai University
2017-2021
Nanjing Drum Tower Hospital
2019-2020
Fundación Juan March
2020
Creative Commons
2020
The mitochondrial Ca2+ uniporter complex (MCUC) is responsible for influx into the matrix, playing critical roles in various functions. Eukaryotic MCUC consists of multiple subunits, and its activity controlled by regulatory including uptake 1 (MICU1) paralogs (MICU2 MICU3). However, underlying mechanism remains unclear. Here, we determined crystal structures MICU2 MICU3 from Homo sapiens. Our data demonstrate that distinct MICU protein N-domains determine specific type dimers perform...
The Orai channel is characterized by voltage independence, low conductance, and high Ca2+ selectivity plays an important role in influx through the plasma membrane (PM). How activated promotes permeation not well understood. Here, we report crystal structure cryo-electron microscopy (cryo-EM) reconstruction of a Drosophila melanogaster (dOrai) mutant (P288L) that constitutively active according to electrophysiology. open state showed hexameric assembly which 6 transmembrane 1 (TM1) helices...
Store-operated calcium entry (SOCE) is a major pathway for ions influx into cells and has critical role in various cell functions. Here we demonstrate that calcium-bound calmodulin (Ca2+-CaM) binds to the core region of activated STIM1. This interaction facilitates slow Ca2+-dependent inactivation after Orai1 channel activation by wild-type STIM1 or constitutively active mutant. We define CaM-binding site STIM1, which adjacent STIM1-Orai1 coupling region. The binding Ca2+-CaM disrupts...
Animals defend a target level for their fundamental needs, including food, water, and sleep. Deviation from the range, or "setpoint," triggers motivated behaviors to eliminate that difference. Whether how setpoint itself is encoded remains enigmatic all behaviors. Employing high-throughput feeding assay in Drosophila, we demonstrate protein intake set different values male, virgin female, mated female flies meet varying demands. Leveraging this variability, found, remarkably, information on...
In the treatment of coronary artery disease (CAD), use stent implantation often leads to clinical complications such as restenosis, delayed endothelial healing, and thrombosis. Here, we develop a double drug sustained-release coating for surface by grafting heparin/NONOate nanoparticles (Hep/NONOates). The Hep/NONOates modification were characterized X-ray photoelectron spectroscopy, attenuated total reflection Fourier-transform infrared static water contact angle, scanning electron...
l-Fucose (Fuc), a six-deoxy hexose monosaccharide, is present endogenously in humans and animals has wide range of biological functions. In the study, we aimed to examine effect Fuc on obesity hepatic steatosis mice fed high-fat diet (HFD). C57BL/6 were normal chow (NC) or HFD for 18 weeks induce fatty liver. was administered intragastrically from 8th week end experiment (18 weeks). Metagenomic analysis showed that altered genomic profile gut microbiota mice; specifically, expression...
Bioresorbable vascular scaffolds are expected to replace the traditional metal stent, avoiding long-term complications of stents. However, it is hard for scaffold manufacturing process meet requirements individualized treatment lesions, which requires different morphologies. Here, we used a new method manufacturing, three-dimensional printing technology, prepare bioresorbable scaffolds. The fabricated was loaded with sirolimus mixed preparation material slow drug release. engineered, drug-...
Abstract Background We previously found that the intestinal epithelial chemokine (C-C motif) ligand 7 (CCL7) plays an important role in development of toxin-induced acute liver damage. The detailed effects CCL7 on chronic diseases; however, are still unclear. Here, we aimed to investigate impact overexpression high-fat diet (HFD)-induced obesity and steatohepatitis mice. Methods Intestinal (CCL7 tgIEC ) mice their wild-type (WT) littermates were fed with normal chow or HFD for 16 weeks...
Objective To observe the effects of islet amyloid polypeptide (IAPP) on KATP channel in rat cell tumor line Ins-1 stimulated by high concentration glucose. Methods Ins-1 cells, as research object, were randomly divided into 16.7 mmol/L glucose control group and 0.1, 1, 10 μmol/L amylin pretreatment group. Whole patch clamp technique was employed to study influences short exposure IAPP electrophysiological characteristics upon stimulation. Meanwhile, insulin secretion measured...
The Orai channel is characterized by voltage independence, low conductance and high Ca2+ selectivity plays an important role in influx through the plasma membrane. How activated promotes permeation are not well understood. Here, we report crystal structure cryo-electron microscopy reconstruction of a Drosophila melanogaster mutant (P288L) that constitutively active according to electrophysiology. open state showed hexameric assembly which six TM1 helices center form ion-conducting pore, TM4...
The mitochondrial Ca2 uniporter complex (MCUC) is responsible for influx into the matrix, playing critical roles in various functions. Eukaryotic MCUC consists of multiple subunits and its activity controlled by regulatory subunits, including uptake 1 (MICU1) paralogs (MICU2 MICU3). However, underlying mechanism remains unclear. Here, we determined crystal structures MICU2 MICU3 from Homo sapiens. Our structural analysis together with cell biology data demonstrate that distinct MICU protein...