A5005284029- Pregnancy and preeclampsia studies
- Angiogenesis and VEGF in Cancer
- Heme Oxygenase-1 and Carbon Monoxide
- Neonatal Respiratory Health Research
- Birth, Development, and Health
- Immune cells in cancer
- CRISPR and Genetic Engineering
- Single-cell and spatial transcriptomics
- Renal Transplantation Outcomes and Treatments
- Virus-based gene therapy research
- Blood Coagulation and Thrombosis Mechanisms
- Eicosanoids and Hypertension Pharmacology
- Dialysis and Renal Disease Management
- Retinoids in leukemia and cellular processes
- Neuroinflammation and Neurodegeneration Mechanisms
- Xenotransplantation and immune response
- Erythropoietin and Anemia Treatment
- Muscle and Compartmental Disorders
- Immune Cell Function and Interaction
- Nosocomial Infections in ICU
- Arsenic contamination and mitigation
- Cell Adhesion Molecules Research
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Blood Pressure and Hypertension Studies
- Pain Management and Opioid Use
University of Alabama at Birmingham
2021-2024
Pediatric Nephrology of Alabama
2021-2024
Tampa General Hospital
2024
Massachusetts General Hospital
2023
Birmingham VA Medical Center
2023
Jackson Memorial Hospital
2019-2021
University of Mississippi Medical Center
2017-2021
The kidney contains a population of resident macrophages from birth that expands as it grows and forms contiguous network throughout the tissue. Kidney-resident (KRMs) are important in homeostasis response to acute injury. While many microenvironments, is unknown whether KRMs heterogeneous differentiated by function location. We combined single-cell RNA-Seq (scRNA-Seq), spatial transcriptomics, flow cytometry, immunofluorescence imaging localize, characterize, validate KRM populations during...
Abstract Pig-to-human xenotransplantation is rapidly approaching the clinical arena; however, it unclear which immunomodulatory regimens will effectively control human immune responses to pig xenografts. Here, we transplant a gene-edited kidney into brain-dead recipient on pharmacologic immunosuppression and study response xenograft using spatial transcriptomics single-cell RNA sequencing. Human cells are uncommon in porcine cortex early after consist of primarily myeloid cells. Both...
Obesity and increased lipid availability have been implicated in the development progression of chronic kidney disease. One major sites renal accumulation is proximal tubule cells kidney, suggesting that these may be susceptible to lipotoxicity. We previously demonstrated loss hepatic biliverdin reductase A (BVRA) causes fat livers mice on a high-fat diet. To determine role BVRA mouse cells, we generated CRISPR targeting for knockout (BVRA KO). The KO had significantly less metabolic...
Biliverdin reductase (BVR) is the enzyme responsible for last step in production of bilirubin from breakdown heme. Bilirubin one most potent antioxidant molecules body. Monitoring BVR activity essential studying capacity cells and tissues. Traditional methods determining have relied on measurement converted biliverdin using absorbance spectroscopy. The approach has limited sensitivity requires large quantities or We developed a novel fluorescence-based method utilizing eel protein, UnaG,...
AKI is a common sequela of infection with SARS-CoV-2 and contributes to the severity mortality from COVID-19. Here, we tested hypothesis that kidney alterations induced by COVID-19-associated could be detected in cells collected urine.
The human placenta is of vital importance for proper nutrient and waste exchange, immune regulation, overall fetal health growth. Specifically, the extracellular matrix (ECM) placental syncytiotrophoblasts, which extends outward from chorionic villi into maternal blood, acts on a molecular level to regulate maintain this barrier. Importantly, barrier dysfunction has been linked diseases pregnancy such as preeclampsia intrauterine growth restriction. To help facilitate our understanding...
Induction of heme oxygenase-1 (HO-1) has been demonstrated to decrease body weight and improve insulin sensitivity in several models obesity rodents. To further study the role HO-1 adipose tissue, we created an adipose-specific knockout mouse model. Male female mice were fed either a control or high-fat diet for 30 weeks. Body weights measured weekly composition, fasting blood glucose levels determined every six Adipocyte-specific had no significant effect on but increased normal-fat diet....
Abstract Soluble vascular endothelial growth factor receptor-1 (sFlt-1) is an anti-angiogenic protein which secreted by numerous cell types and acts as a decoy receptor for the angiogenic (VEGF). Despite its physiologic importance in maintaining balance, excess sFlt-1 levels are associated with pathogenesis of many diseases, especially those imbalance, dysfunction, hypertension. Although soluble protein, it contains binding site extracellular matrix component heparan sulfate. This allows...
Resident memory T cells (T
Our physiological model replicates clinical trial results and provides unique insights into possible mechanisms that play a role in glomerular injury hypertensive kidney disease progression during chronic calcium channel blocker (CCB) therapy. Specifically, these simulations predict the temporal changes renal function with CCB treatment demonstrate important roles for tubuloglomerular feedback efferent arteriolar conductance control of progression.
Arsenicals are deadly chemical warfare agents which primarily cause death through systemic capillary fluid leakage and hypovolemic shock. Arsenical exposure is also known to acute kidney injury, a condition that contributes arsenical-associated due the necessity of in maintaining whole-body homeostasis. Because global health risk arsenicals pose, nuanced understanding how arsenical can lead injury needed. We utilized non-targeted transcriptional approach evaluate effects cutaneous...
Abstract Pig-to-human xenotransplantation is rapidly approaching the clinical arena; however, it unclear which immunomodulatory regimens will effectively control human immune responses to pig xenografts. We transplanted a gene-edited kidney into brain-dead recipient on pharmacologic immunosuppression and studied response xenograft using spatial transcriptomics single-cell RNA sequencing. Human cells were uncommon in porcine cortex early after consisted of primarily myeloid cells. Both...
Acute kidney injury (AKI) is a serious complication of rhabdomyolysis that significantly impacts survival. Myoglobin released from the damaged muscle accumulates in kidney, causing heme iron-mediated oxidative stress, tubular cell death, and inflammation. In response to injury, myeloid cells, specifically neutrophils macrophages, infiltrate kidneys, mediate injury. Ferritin, comprised ferritin light chain heavy (FtH), vital for intracellular iron handling. Given dominant role macrophages...
Resident memory T cells (T RM s) are a subset of which retained locally within barrier and epithelial tissues such as the kidney have also been noted for their role in perpetuating chronic inflammation. While gained recognition ability to modulate inflammation various models injury s still poorly understood contributions sterile disease (CKD) unknown. We hypothesized that activated setting CKD. utilized an aristolochic acid (AA) model murine CKD investigate presence via flow cytometry...
In this issue of The Journal Physiology, Shen et al. describe how neuropilin-1 mediates juxtaglomerular cell (JGC) fate and renin production (Shen al., 2024). Renin is an enzyme that catalyses the conversion angiotensinogen to angiotensin I, a key step in renin–angiotensin–aldosterone system's regulation kidney sodium reabsorption, glomerular filtration rate (GFR) blood pressure. Granules within JGCs, which are modified vascular smooth muscle cells nephron, store release into circulation via...
Preeclampsia (PE) is one of the most common complications pregnancy, affecting ~5-7% pregnancies. Hallmarked by new onset hypertension and maternal endothelial dysfunction, PE believed to be caused insuffcient blood flow placenta. This causes release factors into circulation; notably soluble VEGF receptor sFlt-1, which antagonizes effects in endothelium. Placental growth factor (PlGF) a family member produced placenta that only binds Flt-1 has little inherent angiogenic activity. We...
Curr Pain Headache . Rep 2002 Feb;6(1):52‐56 The design and execution of clinical trials poses special problems for cluster headache. Although there is less inter‐individual intra‐individual variability attacks than seen with migraine, the brevity attacks, spontaneous remissions unrelated to treatment, relative rarity headaches challenge investigators. International Society has developed guidelines that represent a compromise between scientific rigor practicality. Only injectable sumatriptan...
Melkonian, Arin; Moore, Kyle H.; Traylor, Amie; Black, Laurence M.; Zmijewska, Anna A.; Esman, Stephanie; Srivastava, Ritesh; Athar, Mohammad; George, James F.; Agarwal, Anupam Author Information
Obesity and increased lipid availability have been implicated in the development progression of chronic kidney disease. The proximal tubule cells are major site accumulation kidney, thus a target for toxic effect excessive –lipotoxicity. aim current study was to test hypothesis that loss biliverdin reductase‐A (BVRA) increases susceptibility mouse excess lipotoxicity. We used CRISPR‐Cas9 technology generate BVRA knock out (BVRA‐KO) cell line from wildtype (WT) immortalized MCT cells. exposed...
Preeclampsia (PE) is a hypertensive disorder of pregnancy which affects ~5–7% all pregnancies in the United States. Hallmarked by new‐onset hypertension and organ damage, it leading cause premature birth maternal/fetal perinatal morbidity. While PE remains elusive, placental dysfunction due to chronic ischemia believed be central regulator disorder. One factors known released from ischemic placenta soluble form VEGF receptor Flt‐1 (sFlt‐1), into maternal circulation major contributor...