A5065026925- Reproductive System and Pregnancy
- Pregnancy and preeclampsia studies
- Frailty in Older Adults
- Immune Response and Inflammation
- IL-33, ST2, and ILC Pathways
- Cancer-related cognitive impairment studies
- Immune Cell Function and Interaction
- Cellular transport and secretion
- Nutrition and Health in Aging
- Lung Cancer Treatments and Mutations
- Pregnancy and Medication Impact
- Immune cells in cancer
- Neonatal and fetal brain pathology
- Preterm Birth and Chorioamnionitis
- Fetal and Pediatric Neurological Disorders
- Myasthenia Gravis and Thymoma
- interferon and immune responses
- Glioma Diagnosis and Treatment
- Health Systems, Economic Evaluations, Quality of Life
- Pancreatic and Hepatic Oncology Research
- Cancer survivorship and care
- Brain Metastases and Treatment
- RNA modifications and cancer
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Neurobiology and Insect Physiology Research
University of Wisconsin–Madison
2016-2024
City of Hope
2018-2024
University of California, Davis
2017-2024
City Of Hope National Medical Center
2018-2024
The University of Texas at Austin
2024
Memorial Sloan Kettering Cancer Center
2022
National Cancer Center
2021
Swiss Cancer Center Léman
2020
École Polytechnique Fédérale de Lausanne
2014-2018
University of Wisconsin System
2016
Abstract Neutrophils are an essential part of the innate immune system. To study their importance, experimental studies often aim to deplete these cells, generally by injecting anti-Ly6G or anti-Gr1 antibodies. However, approaches only partially effective, transient lack specificity. Here we report that neutrophils remaining after treatment newly derived from bone marrow, instead depletion escapees. Mechanistically, generated, circulating have lower Ly6G membrane expression, and consequently...
Alterations in glucose metabolism and epithelial-mesenchymal transition (EMT) constitute two important characteristics of carcinoma progression toward invasive cancer. Despite an extensive characterization each them separately, the links between EMT tumor cells remain elusive. Here we show that neuronal transporter GLUT3 contributes to uptake proliferation lung have undergone EMT. Using a panel human non-small cell cancer (NSCLC) lines, demonstrate is strongly expressed mesenchymal, but not...
Older adults with cancer facing competing treatments must prioritize between various outcomes. This study assessed health outcome prioritization among older starting chemotherapy.
Research Article19 September 2017Open Access Source DataTransparent process Mutant CTNNB1 and histological heterogeneity define metabolic subtypes of hepatoblastoma Stefania Crippa Swiss Institute for Experimental Cancer Research, School Life Sciences, Ecole Polytechnique Fédérale de Lausanne, Switzerland Search more papers by this author Pierre-Benoit Ancey Jessica Vazquez Paolo Angelino Bioinformatics Core Facility, SIB Bioinformatics, Anne-Laure Rougemont Division Clinical Pathology,...
The purpose of this study was to evaluate longitudinal changes in brain gray matter density (GMD) before and after adjuvant chemotherapy older women with breast cancer.We recruited 16 aged ≥ 60 years stage I-III cancers receiving (CT) 15 age- sex-matched healthy controls (HC). CT group underwent MRI the NIH Toolbox for Cognition testing prior (time point 1, TP1) within 1 month 2, TP2). HC same assessments at matched intervals. GMD evaluated voxel-based morphometry.The mean age 67 68.5 group....
Defects in genes encoding the isoforms of laminin alpha subunit have been linked to various phenotypic manifestations, including brain malformations, muscular dystrophy, ocular defects, cardiomyopathy, and skin abnormalities. We report here a severe defect neuromuscular transmission consanguineous patient with homozygous variant alpha‐5 gene ( LAMA5) . The c.8046C>T (p.Arg2659Trp) is rare has predicted deleterious effect. affected individual, who also carries sequence LAMA1 , had muscle...
NBSGW mice are highly immunodeficient and carry a hypomorphic mutation in the c-kit gene, providing host environment that supports robust human hematopoietic expansion without pre-conditioning. These thus provide model to investigate engraftment absence of conditioning-associated damage. We compared transplantation CD34+ HSPCs purified from three different sources: umbilical cord blood, adult bone marrow G-CSF mobilized peripheral blood. blood were significantly more efficient (as function...
Adaptive immune system, principally governed by the T cells - dendritic (DCs) nexus, is an essential mediator of gestational fetal tolerance and protection against infection. However, exact composition dynamics DCs cell subsets in tissues are not well understood. These controlled human physiology a complex interplay alloantigen distribution presentation, cellular/humoral active passive tolerance, hormones/chemokines/angiogenic factors their gradients, systemic local microbial communities....
A successful pregnancy requires many physiological adaptations from the mother, including establishment of tolerance towards semiallogeneic fetus. Innate lymphoid cells (ILCs) have arisen as important players in immune regulation and tissue homeostasis at mucosal barrier surfaces. Dimensionality reduction transcriptomic analysis revealed presence two novel CD56Bright decidual ILCs that express low T-bet divergent Eomes levels. These persist across we found their transcriptome correlates...
Problem Decidual immune dysregulation is thought to underlie major pregnancy disorders; however, incomplete understanding of the decidual interface has hampered mechanistic investigation. Method study Human term decidua was collected, and single‐cell phenotypic information acquired by highly polychromatic flow cytometry. Cellular identity analysis performed with t‐distributed stochastic neighbor embedding, Dens VM clustering, matched CellOntology database. Results Traditional analytical...
Recently, dynamic contrast-enhanced (DCE) MRI with ferumoxytol as contrast agent has recently been introduced for the noninvasive assessment of placental structure and function throughout. However, it not demonstrated under pathological conditions.
Summary Neutrophils orchestrate the innate immune response against microorganisms and are increasingly recognized to modulate cancer development in primary tumors metastases. To address their function vivo , different approaches used, most common ones relying on antibody-mediated neutrophil depletion. By comparing effects of two widely used antibodies, we demonstrate a strong efficacy but lack specificity for anti-Gr1. In contrast, anti-Ly6G lacks neutrophil-depletion capacity C57BL/6 mice,...
The enzyme choline acetyltransferase (ChAT) synthesizes acetylcholine from acetyl-CoA and at the neuromuscular junction nerve terminals of cholinergic neurons. Mutations in ChAT gene (CHAT) result a presynaptic congenital myasthenic syndrome (CMS) that often associates with life-threatening episodes apnea. Knockout mice for Chat (Chat-/-) die birth. To circumvent lethality this model, we crossed mutant possessing loxP sites flanking exons 4 5 expressed Cre-ERT2. Injection tamoxifen (Tx)...
In congenital stationary night blindness, type 2 (CSNB2)—a disorder involving the Ca v 1.4 (L-type) 2+ channel—visual impairment is mild considering that mediates synaptic release from rod and cone photoreceptors. Here, we addressed this conundrum using a knockout (KO) mouse knock-in (G369i KI) expressing non-conducting 1.4. Surprisingly, 3 (T-type) currents were detected in cones of G369i KI mice KO but not wild-type mouse, ground squirrels, macaque retina. Whereas are blind, exhibit normal...
Placental blood flow is a marker that reflects the health of utero-placental vasculature. Dynamic contrast-enhanced (DCE) MRI more robust than arterial spin labeling and can be utilized in animal models to provide cotyledon-specific volume measurements vivo throughout gestation. This study investigated distribution placental at cotyledon level pregnant rhesus monkeys before following injection Tisseel (a fibrin sealant) MCP1 (an inflammatory response inducer) assess predictabilities for injury.
In congenital stationary night blindness, type 2 (CSNB2)—a disorder involving the Ca v 1.4 (L-type) 2+ channel—visual impairment is mild considering that mediates synaptic release from rod and cone photoreceptors. Here, we addressed this conundrum using a knockout (KO) mouse knock-in (G369i KI) expressing non-conducting 1.4. Surprisingly, 3 (T-type) currents were detected in cones of G369i KI mice KO but not wild-type mouse, ground squirrels, macaque retina. Whereas are blind, exhibit normal...
Monogenic defects of synaptic vesicle (SV) homeostasis have been implicated in many neurologic diseases, including autism, epilepsy, and movement disorders. In addition, abnormal exocytosis has associated with several endocrine dysfunctions.We report an 11 year old girl learning disabilities, tremors, ataxia, transient hyperglycemia, muscle fatigability responsive to albuterol sulfate. Failure neuromuscular transmission was confirmed by single fiber electromyography. Electron microscopy...