A5100692724- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience of respiration and sleep
- Neuropeptides and Animal Physiology
- Immune Response and Inflammation
- Neuroendocrine regulation and behavior
- Neurological diseases and metabolism
- Stress Responses and Cortisol
- Anesthesia and Neurotoxicity Research
- Gastrointestinal motility and disorders
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Metabolism and Genetic Disorders
- Neurotransmitter Receptor Influence on Behavior
- Receptor Mechanisms and Signaling
- Circular RNAs in diseases
- Infant Health and Development
- Vagus Nerve Stimulation Research
- Mitochondrial Function and Pathology
- Neurological Disease Mechanisms and Treatments
- Nitric Oxide and Endothelin Effects
- Hydrogen's biological and therapeutic effects
- Pain Mechanisms and Treatments
- Neonatal and fetal brain pathology
- S100 Proteins and Annexins
- Child Nutrition and Feeding Issues
Xuzhou Medical College
2016-2025
North China University of Technology
2024
National Medical Products Administration
2022-2024
Zunyi Medical University
2023
Inner Mongolia Medical University
2021
Shanxi Provincial Cancer Hospital
2021
Shanxi Medical University
2021
Baoshan College
2018
St. Jude Children's Research Hospital
2007-2017
Weifang People's Hospital
2017
Microglia play an important role in neuronal protection and damage. However, the molecular cellular relationship between microglia neurons is unclear. We carried out a prospective study to detect that activation of BV2 induced PC12 cell apoptosis vitro through TLR4/adapter protein myeloid differentiation factor 88 (MyD88)/nuclear factor-κB (NF-κB) signaling pathway. were treated with different concentrations LPS for 24 h. Western blot was utilized expression TLR4 downstream The level...
The molecular details that govern the specific interactions between acyl carrier protein (ACP) and enzymes of fatty acid biosynthesis are unknown. We investigated mechanism ACP·protein using a computational analysis to dock NMR structure ACP with crystal β-ketoacyl-ACP synthase III (FabH) experimentally tested model by biochemical FabH mutants. activities mutants were assessed both an ACP-dependent ACP-independent assay. interaction surface was defined mutations compromised activity in assay...
Pituitary adenylate cyclase-activating polypeptide (PACAP) is effective in reducing axonal damage associated with traumatic brain injury (TBI), and has immunomodulatory properties. Toll-like receptor 4 (TLR4) an important mediator of the innate immune response. It significantly contributes to neuroinflammation induced by injury. However, it remains unknown whether exogenous PACAP can modulate TBI through TLR4/adapter protein myeloid differentiation factor 88 (MyD88)/nuclear factor-κB (NF-κB)...
Pantothenate kinase (PanK) catalyzes the first step in CoA biosynthesis and there are three human genes that express four isoforms with highly conserved catalytic core domains. Here we report homodimeric structures of cores PanK1alpha PanK3 complex acetyl-CoA, a feedback inhibitor. Each monomer adopts fold actin superfamily inhibitor-bound explain basis for allosteric regulation by thioesters. These also provide an opportunity to investigate structural effects PanK2 mutations have been...
Pantothenate kinase (PanK) phosphorylates pantothenic acid (vitamin B5) and controls the overall rate of coenzyme A (CoA) biosynthesis. Pank1 gene deletion in mice results a metabolic phenotype where fatty oxidation gluconeogenesis are impaired fasted state, leading to mild hypoglycemia. Inactivating mutations human PANK2 lead childhood neurodegeneration, but Pank2 inactivation does not elicit indicative neuromuscular symptoms or brain iron accumulation that accompany disease. Pank1/Pank2...
Abstract Objectives Previously we demonstrated the neuroprotective effect of montelukast, a cysteinyl leukotriene receptor-1 (CysLT1) antagonist, on acute brain injury after focal cerebral ischaemia in mice. In this study, have determined its chronic mice and rats. Methods After transient was induced by middle artery occlusion, montelukast intraperitoneally injected or orally administered to rats for five days. Behavioural dysfunction, infarct volume, atrophy neuron loss were evaluate...
Background/Aims: Ketamine has been reported to exert anti-inflammatory effects on astrocytes stimulated by lipopolysaccharide (LPS) in vitro and vivo. However, the mechanism not elicited clearly. The aim of this study was investigate ketamine TLR4 expression NF-ĸB-p65 phosphorylation, as well production proinflammatory cytokines LPS challenged astrocytes. Methods: Astrocytes were with (1µg/ ml) absence presence various concentrations (10, 100, 1000µM). IL-1β, IL-6 TNF-α measured ELISA, glial...
The increasing prescription of opioids is fueling an epidemic addiction and overdose deaths. Morphine a highly addictive drug characterized by high rate relapse – even after long periods abstinence. Serotonin (5-HT) neurotransmission participates in the development morphine dependence, as well expression withdrawal. In this study, we examined effect blockade 5-HT2A receptors (5-HT2ARs) on morphine-induced behavioral sensitization withdrawal male mice. 5-HT2AR antagonist MDL 11,939 (0.5...
Neonatal maternal separation (MS) is a major early-life stress that increases the risk of emotional stress, visceral pain perception and other brain disorders. Elevation toll-like receptor 4 (TLR4) signaling in paraventricular nucleus (PVN) precipitates colorectal distension (CRD)-induced hypersensitivity adulthood. The present study aimed to investigate role TLR4 pathogenesis postnatal MS-induced during gene was selectively knocked out C57BL/10ScSn mice (Tlr4−/−). MS developed by housing...
Visceral hypersensitivity as a common clinical manifestation of irritable bowel syndrome (IBS) may contribute to the development chronic visceral pain. Our prior studies authenticated that activation corticotropin-releasing factor (CRF) neurons in paraventricular nucleus (PVN) contributed mice, but puzzles still remain with respect underlying hyperactivation neurons. Herein, we employed maternal separation (MS) establish mouse model hypersensitivity. The neuronal circuits associated...
AbstractObject: The neuroprotective effects of pituitary adenylate cyclise-activating polypeptide (PACAP) have been well documented in vivo and vitro. However, the mechanisms by which PACAP protected microglia from ischemic/hypoxic injury via inhibition activation remain unclear. Toll-like receptor 4 (TLR4) plays a considerable role induction innate immune inflammatory responses. purpose this study is to investigate effect on oxygen glucose deprivation (OGD)/reoxygenation BV2 explore...
Summary Patients with chronic hepatitis B virus ( HBV ) infection and normal or mildly increased transaminases may have sustained significant liver damage, as verified by biopsy. However, no suitable noninvasive method exists for identifying necroinflammation in such patients. We aimed to investigate the power of micro RNA ‐124 a novel biomarker necroinflammation. A total 131 recruited patients underwent biopsy grading (G) staging fibrosis (S). Thirty healthy individuals were included...
Prostate cancer (PCa) is the second leading contributor to male malignancy-associated death in developed countries. The study aimed evaluate effects of miR-106a on prostate cells and underlying molecular mechanism.The cell proliferation was detected using MTT assay colony formation, migration invasion were also analyzed. Luciferase reporter carried out explore correlation between PTEN. Besides, reverse transcriptase-polymerase chain reaction western blot measure mRNA protein levels tissues...
Abstract Exposure to moderate level of stress during the perinatal period helps organisms cope well with stressful events in their later life, an effect known as inoculation. Amygdala is one kernel brain regions mediating stress-coping brain. However, little about whether early life may affect amygdala have its inoculative effect. Here, we observed that maternal separation (MS) from postnatal day 3 21 (D3–21, 1 h per day) significantly alleviated increased anxiety-like behavior induced by...