A5062351846- Pluripotent Stem Cells Research
- Zebrafish Biomedical Research Applications
- Drug Transport and Resistance Mechanisms
- CRISPR and Genetic Engineering
- Glioma Diagnosis and Treatment
- Single-cell and spatial transcriptomics
- Phagocytosis and Immune Regulation
- Epigenetics and DNA Methylation
- Cancer therapeutics and mechanisms
- Acute Myeloid Leukemia Research
- Renal and related cancers
- IL-33, ST2, and ILC Pathways
- CAR-T cell therapy research
- Erythrocyte Function and Pathophysiology
- Immune cells in cancer
- Pharmacological Effects and Toxicity Studies
- Mesenchymal stem cell research
- Immune Cell Function and Interaction
- RNA Interference and Gene Delivery
- Cell Adhesion Molecules Research
- Protein Degradation and Inhibitors
- Occupational Health and Performance
- Natural product bioactivities and synthesis
- Kruppel-like factors research
- Autoimmune and Inflammatory Disorders Research
Institute of Hematology & Blood Diseases Hospital
2017-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2017-2025
Second Affiliated Hospital of Zhejiang University
2024
China-Japan Friendship Hospital
2013-2022
Johns Hopkins Medicine
2019
Johns Hopkins University
2019
Bayer (United States)
2009-2019
Guangzhou Medical University
2019
Guangzhou Women and Children Medical Center
2019
St. Jude Children's Research Hospital
2008-2010
Brain edema is an important factor leading to morbidity and mortality associated with primary brain tumors. Dexamethasone, a synthetic glucocorticoid, routinely prescribed antineoplastic agents alleviate pain chemotherapy reduce intracranial pressure. We investigated whether dexamethasone treatment increased the expression activity of multidrug resistance (MDR) transporters at blood-brain barrier. Treatment rat microvascular endothelial cells submicromolar concentrations induced...
Replacement therapy for hemophilia remains a lifelong treatment. Only gene can cure at fundamental level. The clustered regularly interspaced short palindromic repeats–CRISPR associated nuclease 9 (CRISPR-Cas9) system is versatile and convenient genome editing tool which be applied to hemophilia. A patient's induced pluripotent stem cells (iPSCs) were generated from their peripheral blood mononuclear (PBMNCs) using episomal vectors. AAVS1-Cas9-sgRNA plasmid targets the AAVS1 locus...
Gefitinib, an epidermal growth factor receptor tyrosine kinase inhibitor, increases brain parenchymal extracellular fluid (ECF) accumulation of topotecan, a substrate the ATP-binding cassette (ABC) transporters P-glycoprotein (Pgp/MDR-1) and breast cancer resistance protein (BCRP/ABCG2). The effect modulating these on topotecan penetration in gliomas has not been thoroughly studied. Thus, we performed intracerebral microdialysis mice bearing orthotopic human (U87 MT330) assessed tumor ECF...
We tested the assumption that nifedipine blocks L-type calcium current [I(Ca(L))] at +10 mV and unmasks Na(+)/Ca(2+) exchange-triggered contractions in guinea pig isolated ventricular myocytes. Voltage-clamp pulses elicited I(Ca(L)) evoked myocytes superfused with Tyrode's solution (35 degrees C). Nifedipine blocked an IC(50) of 0.3 microM; this decreased to 50 nM a holding potential -40 mV, indicating preferential block inactivated Ca(2+) channels. Use-independent increased concentration...
Abstract Objectives Vitronectin (VTN) has been widely used for the maintenance and expansion of human pluripotent stem cells (hPSCs) as feeder‐free conditions. However, effect VTN on hPSC differentiation remains unclear. Here, we investigated role in early haematopoietic development hPSCs. Materials Methods A chemically defined monolayer system was applied to study different matrix or basement membrane proteins The integrin signalling VTN‐mediated by antagonists. Finally, small interfering...
Lipoxin A4 (LXA4) is a structurally and functionally distinct natural product called an eicosanoid, which displays immunomodulatory anti-inflammatory activity but rapidly metabolized to inactive catabolites in vivo. A previously described analogue of LXA4, methyl (5R,6R,7E,9E,11Z,13E,15S)-16-(4-fluorophenoxy)-5,6,15-trihydroxy-7,9,11,13-hexadecatetraenoate (2, ATLa), was shown have poor pharmacokinetic profile after both oral intravenous administration, as well sensitivity acid light. The...
Topotecan is a substrate of the ATP-binding cassette transporters P-glycoprotein (P-gp/MDR1) and breast cancer resistance protein (BCRP). To define role these in topotecan penetration into ventricular cerebrospinal fluid (vCSF) brain parenchymal extracellular (ECF) compartments, we performed intracerebral microdialysis on transporter-deficient mice after an intravenous dose (4 mg/kg). vCSF unbound lactone was measured as ratio vCSF-to-plasma area under concentration-time curves. The mean +/-...
Hypoxia enhances hematopoiesis with functional T cell potential from human pluripotent stem cells via arterial priming.
The ATP-binding cassette transporters P-glycoprotein (ABCB1, MDR1) and multidrug resistance protein 4 (MRP4) efflux irinotecan its active metabolite SN-38 in vitro, thus may contribute to system clearance of these compounds. Mdr1a/b-/-, Mrp4-/-, wild-type mice were administered 20 or 40 mg/kg irinotecan, plasma samples collected for 6 hours. Irinotecan lactone carboxylate quantitated data analyzed with nonlinear mixed-effects modeling. Mdr1a/b genotype was a significant covariate the both...
Heat‐shock protein‐90 is an attractive target for anticancer drugs, as heat‐shock blockers such the ansamycin 17‐(allylamino)‐17‐demethoxygeldanamycin greatly reduce expression of many signaling molecules that are disregulated in cancer cells and key drivers tumor growth metastasis. While has shown promise clinical trials, this compound class significant template‐related drawbacks. In paper, we describe a new, potent non‐ansamycin small‐molecule inhibitor protein‐90, BX‐2819, containing...
Distinct regions of the primitive streak (PS) have diverse potential to differentiate into several tissues, including hematopoietic lineage originated from posterior region PS. Although various signaling pathways been identified promote development PS and its mesoderm derivatives, there is a large gap in our understanding that regulate fate Here, we defined roles Wnt, activin, bone morphogenetic protein (BMP) generating hematopoietic-fated human pluripotent stem cells (hPSCs). We found...
Highlights•IL-33 mobilizes hematopoietic stem and progenitor cells.•Cells include stem, lymphoid, endothelial cells.•Long-acting, oxidation-resistant IL-33 can be engineered.•A single dose of this engineered is sufficient for cell mobilization.•Use may represent a novel approach to improve bone marrow transplantation.ABSTRACTMobilization cells (HSPCs) has become increasingly important transplantation. Current mobilization approaches are insufficient because they fail mobilize numbers in...
Mantle Cell Lymphoma (MCL) is typically an aggressive and rare disease with poor prognosis, therefore new effective therapeutics are urgently needed. Drug repurposing for cancer treatment becoming increasingly more attractive as alternative approach to discover clinically approved drugs that demonstrate antineoplastic effect. The objective of this study was screen drug library identify candidate compounds effect in MCL cells using High-Throughput Screening (HTS) technique.Using the HTS...
1-(2-deoxy-2-fluoro-4-thio-beta-D-arabinofuranosyl) cytosine (4'-thio-FAC) is a deoxycytidine analog that has been shown previously to have impressive anti-proliferative and cytotoxic effects in vitro vivo toward colorectal gastric tumors. In our present studies, the pharmacokinetic behavior nude mice effectiveness of 4'-thio-FAC against human pancreatic ovarian tumor growth were assessed comparison with standard chemotherapeutic agents. Potent observed (Capan-1, MIA-PaCa-2, BxPC-3)...
Skewed X chromosome inactivation (XCI) is a rare reason for hemophilia B in females. It indefinite whether reactivation (XCR) would occur when cells of patients with skewed XCI were reprogrammed into induced pluripotent stem (iPSCs). In this study, we investigated female patient known F9 gene mutation: c.676C>T, p.Arg226Trp. We demonstrated that was the pathogenesis patient, and successfully generated numerous iPSC colonies from peripheral blood mononuclear (PBMNCs), which first time...