A5018666090- Retinal Diseases and Treatments
- Retinal and Optic Conditions
- Neuroinflammation and Neurodegeneration Mechanisms
- Retinal Imaging and Analysis
- Retinal Development and Disorders
- Ocular Diseases and Behçet’s Syndrome
- Immunotherapy and Immune Responses
- Glaucoma and retinal disorders
- Liver Disease Diagnosis and Treatment
- Cell Adhesion Molecules Research
- Ophthalmology and Visual Health Research
- Diversity and Career in Medicine
- Cancer Research and Treatments
- CNS Lymphoma Diagnosis and Treatment
- Atherosclerosis and Cardiovascular Diseases
- Immune cells in cancer
- Vascular Malformations and Hemangiomas
- Retinal and Macular Surgery
- Adipose Tissue and Metabolism
- Angiogenesis and VEGF in Cancer
- Patient Satisfaction in Healthcare
- Vascular Malformations Diagnosis and Treatment
- Single-cell and spatial transcriptomics
- Vascular Tumors and Angiosarcomas
- Connexins and lens biology
Yonsei University
2025
Severance Hospital
2025
Korea Advanced Institute of Science and Technology
2021-2023
Yeungnam University
2017-2019
Yeungnam University College
2017-2018
Abstract We aimed to characterize the vascular phenotypes of an experimental autoimmune retinal uveitis (EAU) model induced by interphotoreceptor retinoid-binding protein (IRBP) using multimodal imaging techniques. systemically administered IRBP or vehicle adult C57BL/6 mice. Fundus photography, optical coherence tomography (OCT), in vivo live confocal different tracers, OCT angiography (OCTA), and electroretinography (ERG) were performed after immunization. Hematoxylin eosin...
Introduction: The classically defined two retinal microglia layers are distributed in inner and outer plexiform layers. Although there some reports that also superficially located around the ganglion cell layer (GCL) contact with vitreous, has been a lack of detailed descriptions not fully understood yet. Methods: We visualized microglial by using CX3CR1-GFP (C57BL6) transgenic mice both healthy disease conditions including NaIO3-induced degeneration models IRBP-induced auto-immune uveitis...
Chronic kidney disease (CKD) is one of the most common renal diseases manifested by gradual loss function with no symptoms in early stage. The underlying mechanism pathogenesis CKD various causes such as high blood pressure, diabetes, cholesterol, and infection not well understood. In vivo longitudinal repetitive cellular-level observation animal model can provide novel insights to diagnose treat visualizing dynamically changing pathophysiology its progression over time. this study, using...
Nonalcoholic fatty liver disease (NAFLD) is one of the most common chronic diseases closely associated with metabolic system, including obesity and type 2 diabetes. The progression NAFLD advanced fibrosis an increased risk cirrhosis cancer as well various extra-hepatic diseases. Yet, underlying mechanism not fully understood partly due to absence effective high-resolution in vivo imaging methods appropriate animal models recapitulating pathology NAFLD. To improve our understanding about...
Purpose: To establish a custom-built, high-speed 90 frame-per-second laser-scanning confocal microscope for real-time in vivo retinal imaging of individual flowing red blood cells (RBCs) vasculature live mouse model. Methods: Fluorescently labeled RBCs were injected into mice different ages (3 to 62 weeks old). Anti-CD31 antibody conjugated with Alexa Fluor 647 was visualize endothelial (ECs). Longitudinal and cross-sectional intravital ECs performed two strains (C57BL/6 Balb/c) by using the...
Animal models of retinal artery occlusion (RAO) have been widely used in many studies. However, most these studies prefer using a central (CRAO) which is typical global ischemia model the retina, due to technical limitation producing single vessel targeted modeling with real-time imaging. A focal model, such as branch (BRAO), also needed for explaining interactions, including immunological reaction between ischemic retina and adjacent healthy retina. Accordingly, relevant clinical RAO...
The development of choroidal neovascularization (CNV) is a crucial factor in the pathophysiology and prognosis exudative age-related macular degeneration (AMD). Therefore, detection CNV essential for establishing an appropriate diagnosis treatment plan. Current ophthalmic imaging techniques, such as fundus fluorescent angiography optical coherence tomography, have limitations accurately visualizing lesions expressing activity, owing to issues excessive dye leakage with pooling inability...
In dental pulp, diverse types of cells mediate the pulp immunity in a highly complex and dynamic manner. Yet, 3D spatiotemporal changes various pulpal immune dynamically reacting against foreign pathogens during response have not been well characterized. It is partly due to technical difficulty detailed comprehensive cellular-level observation whole intact tooth beyond conventional histological analysis using thin slices. this work, we validated optical clearing technique based on modified...
Longitudinal cellular-level imaging of pancreatic tumor microenvironment at the same site in pancreas preclinical mouse model was achieved by using intravital dual-mode two- photon and confocal microscope combined with window.
Abstract In addition to the classically defined two microglia layers in plexiform layers, a newly discovered retinal layer is superficially located around ganglion cell (GCL) contact with vitreous. We visualized microglial by using CX3CR1-GFP transgenic mice both healthy and disease conditions. As progresses, each gets closer capillary plexus, including superficial, intermediate, deep making inter-glial distances more visible. The GCL has subsets; peripheral (pph) on parenchyma BAM (CNS...
Oral mucosa is a soft tissue lining the inside of mouth, protecting oral cavity from microbiological insults. The mucosal immune system composed diverse types cells that defend against wide range pathogens. pathophysiology various diseases has been studied mostly by ex vivo histological analysis harvested specimens. However, to analyze dynamic cellular processes in mucosa, longitudinal observation single mouse during pathogenesis highly desirable and efficient approach. Herein, utilizing...