A5011870657- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- Metal complexes synthesis and properties
- RNA and protein synthesis mechanisms
- Synthesis and Characterization of Heterocyclic Compounds
- Cancer therapeutics and mechanisms
- HIV/AIDS drug development and treatment
- Molecular Sensors and Ion Detection
- RNA Interference and Gene Delivery
- Click Chemistry and Applications
- Crystallization and Solubility Studies
- Luminescence and Fluorescent Materials
- Chemical synthesis and alkaloids
- Chemical Synthesis and Analysis
- Phytochemistry and biological activities of Ficus species
- Ferrocene Chemistry and Applications
- Molecular Junctions and Nanostructures
- X-ray Diffraction in Crystallography
- Electrochemical Analysis and Applications
- Photochemistry and Electron Transfer Studies
- Synthesis and Biological Evaluation
- Organoboron and organosilicon chemistry
- Mercury impact and mitigation studies
- Synthesis and Properties of Aromatic Compounds
- Synthesis and Reactivity of Heterocycles
Rudjer Boskovic Institute
2014-2024
Greek Rescue Team
2017
University of Zagreb
2016
Using a novel bioinspired osmolyte-based DES, we engineered biocompatible medium for long-term lysozyme storage, resistant to temperature fluctuations.
To explore in greater detail the recently reported rare kinetic differentiation between homo-polymeric and alternating AT-DNA sequences by using sterically restricted phosphonium dyes that form dimers within DNA minor groove, new analogues were prepared which quinolone moiety was kept constant, while size hydrogen bonding properties of rest molecule varied. Structure-activity relationship studies revealed a slight increase length an additional methylene unit results loss AT selectivity, but...
Poly has a tracker: By sterically controlling monomer–dimer equilibria, novel dyes can kinetically differentiate between homo-AT and alternating AT–AT polynucleotides. In this way, the width steric occupation of DNA minor groove be finely probed easily assessed by induced CD signals (see figure). Detailed facts importance to specialist readers are published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset. They made available submitted authors. Please...
The synthesis, photophysical, and electrochemical properties of selectively mono-, bis- tris-dimethylamino- trimethylammonium-substituted bis-triarylborane bithiophene chromophores are presented along with the water solubility singlet oxygen sensitizing efficiency cationic compounds Cat1+ , Cat2+ Cat(i)2+ Cat3+ . Comparison mono-triarylboranes reveals large influence bridging unit on bis-triarylboranes, especially those compounds. Based these preliminary investigations, interactions DNA,...
Abstract A series of novel phenanthridine–nucleobase conjugates were prepared and studied by spectroscopic methods. An analysis 1 H NMR, UV–Vis fluorescence spectra in aqueous media revealed intramolecular aromatic stacking interactions between the phenanthridinium unit nucleobase, due to conformation molecules. Fluorimetric titrations showed that phenanthridinium–nucleobase 8, 10 reference phenanthridine compound 12 form 1:1 non‐covalent complexes with nucleotides water binding constants...
Among novel bis-nucleobase–phenanthridinium conjugates bis-uracil analogue 2 stabilized significantly more effective poly-dA–poly-dT and poly-AH+–poly-AH+ than adenine 3 reference compound 1. For the alternating poly-dAdT–poly-dAdT however, binding preference is lost, pointing to importance of specific interactions uracils with homopolynucleotides containing consecutive adenines.
Adenine containing conjugates 4 and 5 exhibit specific spectroscopic changes two orders of magnitude higher affinity toward poly U than uracil 2 3 the reference compound 1 due to existence interactions between adenine uracil, possibly Watson-Crick hydrogen bonding bases stacked on phenanthridinium moiety.
Abstract N ‐Methyl‐8‐aminophenathridinium–uracil and –adenine conjugates possessing a nucleobase attached at the phenanthridinium 8‐amino group by trimethylene spacer were prepared in form of water‐soluble hydrogensulfate salts. Spectroscopic characteristics reveal formation folded conformations water characterized intramolecular aromatic stacking between unit tethered nucleobase. The 1:1 complexes with either complementary or non‐complementary nucleotides, giving log K s values 1 2 showing...
A phenanthridine-triazolyluracilyl multifunctional ligand, linked by a lysine–glycine peptide, binds to poly rA–poly rU with micromolar affinity and selective fluorescence response.
A series of structurally similar bis-phenanthridinium derivatives, some with uracil at different positions, revealed interactions various polynucleotides. The uniform binding mononucleotides to all studied compounds by "cyclobisintercaland" type indicated that compound–polynucleotide interaction selectivity was the consequence polynucleotide secondary structure and not direct nucleobase recognition. Although affinity fluorimetric response toward ds-DNA/RNA similar, thermal denaturation ICD...
A series of novel hydrazino-based peptidomimetics and analogues comprising N-terminal lysine C-terminal phenanthridinyl-l-alanine were prepared. The presented results demonstrate the up to now unknown possibility finely modulate peptide interactions with DNA/RNA by α-hydrazino group insertion how different positioning two groups in peptides controls binding various double stranded single DNA RNA. All bind 1-10 micromolar affinity ds-DNA/RNA, whereby mode is a combination electrostatic...
New analogs of the commercial asymmetric monomethine cyanine dyes thiazole orange (TO) and homodimer (TOTO) with hydroxypropyl functionality were synthesized their properties in presence different nucleic acids studied. The novel compounds showed strong, micromolar submicromolar affinities to all examined DNA ds-polynucleotides poly rA–poly rU. studied selectivity towards GC-DNA base pairs over AT-DNA, which included both binding affinity a strong fluorescence response. CD titrations...
Copper(II) complexes with cysteine and histidine, amino acids that coordinate copper(II) in human body, were investigated. Cu-His Cu-Cys detected pH range from 5.0 to 9.0 using voltammetric techniques. [CuHis2] complex reduces by two-electron reversible process at ≈−0.40 V, while [CuCys] one-electron quasireversible −0.6 revealing strong adsorption the electrode surface. When both are present solution, new peak appeared −0.5 which corresponded [CuHisCys] reduction. Formation characterization...
Two novel conjugate molecules were designed: pyrene and phenanthridine-amino acid units with a different linker length between the aromatic fragments. Molecular modelling combined spectrophotometric experiments revealed that in neutral acidic buffered water solutions conjugates predominantly exist intramolecularly stacked conformations because of π-π stacking interaction phenanthridine moieties. The investigated systems exhibited pH-dependent excimer formation is significantly red-shifted...
Two structurally similar bis-phenanthridinium–adenine conjugates (equipped with one or two adenines, respectively), exhibiting strong ds-DNA/RNA groove binding, revealed ratiometric fluorescent recognition of alternating AT-DNA respect to other and ss-RNA. Further, CD spectra pattern adenine–bis-phenanthridinium conjugate/polynucleotide complexes strongly depended on polynucleotide secondary structure. Attached adenine was essential for spectrophotometric response, ds-DNA stabilisation...
Interactions of an array nucleic acid structures with a small series benzothiazole ligands (bis-benzothiazolyl-pyridines—group 1, 2-thienyl/2-benzothienyl-substituted 6-(2-imidazolinyl)benzothiazoles—group 2, and three 2-aryl/heteroaryl-substituted 3) were screened by competition dialysis. Due to the involvement DNA:RNA hybrids triplex helices in many essential functions cells, this study’s main aim is detect benzothiazole-based moieties selective binding or spectroscopic response these...