A5082066820- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- Synthesis and biological activity
- Cancer therapeutics and mechanisms
- X-ray Diffraction in Crystallography
- Synthesis and Characterization of Heterocyclic Compounds
- Crystallization and Solubility Studies
- Click Chemistry and Applications
- Sesquiterpenes and Asteraceae Studies
- Metal complexes synthesis and properties
- Synthesis and Biological Evaluation
- RNA Interference and Gene Delivery
- Luminescence and Fluorescent Materials
- Natural product bioactivities and synthesis
- Essential Oils and Antimicrobial Activity
- RNA and protein synthesis mechanisms
- Chemical synthesis and alkaloids
- Phytochemistry and Biological Activities
- Molecular Sensors and Ion Detection
- Supramolecular Self-Assembly in Materials
- Quinazolinone synthesis and applications
- Axial and Atropisomeric Chirality Synthesis
- Surface Chemistry and Catalysis
- Phytochemistry and biological activities of Ficus species
- Lipid Membrane Structure and Behavior
Rudjer Boskovic Institute
2015-2024
Greek Rescue Team
2014-2017
University of Zagreb
2012
Laboratoire des Biomolécules
2012
We report for the first time on interaction of self-assembled perylene bisimide (PBI) dye aggregates with polynucleotides, revealing very high (nM) binding constants and exceptional thermal stabilization double-stranded (ds)-DNA/RNA. Induced CD profiles PBI dimer formed within DNA/RNA grooves show sensitivity steric properties site, suggesting applicability such self-adjusting excitonically coupled dimers sensing secondary structure.
A broad series of homochiral perylene bisimide (PBI) dyes were synthesized that are appended with amino acids and cationic side chains at the imide positions. Self-assembly behavior these ionic PBIs has been studied in aqueous media by UV/Vis spectroscopy, revealing formation excitonically coupled H-type aggregates. The interactions different ds-DNA ds-RNA have explored thermal denaturation, fluorimetric titration circular dichroism (CD) experiments. These strongly stabilized ds-DNA/RNA...
Newly designed and synthesized cyano, amidino acrylonitrile 2,5-disubstituted furane derivatives with either benzimidazole/benzothiazole nuclei have been evaluated for antitumor antimicrobial activity. For potential activity, the compounds were tested in 2D 3D cell culture methods on three human lung cancer lines, A549, HCC827 NCI-H358, MTS cytotoxicity BrdU proliferation assays vitro. Compounds 5, 6, 8, 9 15 proven to be activity high stop of cells. In general, benzothiazole more active...
A DNA-targeting dye exhibited pH-dependent selectivity toward AT-DNA (pH 7) or GC-DNA 5), accompanied by fluorimetric 5) and ICD recognition.
Peptides that adopt β-helix structures are predominantly found in transmembrane protein domains or the lipid bilayer of vesicles. Constructing a structure pure water has been considered difficult without addition membrane mimics. Herein, we report such an example; peptide 1 self-assembles into supramolecular based on charge interactions between individual peptides. Peptide further showed intriguing transitions from small particles to helical fibers time-dependent process. The can be switched...
Newly designed pentacyclic benzimidazole derivatives featuring amino or amido side chains were synthesized to assess their in vitro antiproliferative activity. Additionally, we investigated direct interaction with nucleic acids, aiming uncover potential mechanisms of biological action. These compounds prepared using conventional organic synthesis methodologies alongside photochemical and microwave-assisted reactions. Upon synthesis, the newly derived underwent testing for effects on various...
Abstract Compounds 1 – 3 , composed of two guanidiniocarbonylpyrrole moieties linked by oligoamide bridges and differing in number type basic groups, were prepared. The sites degree protonation depend strongly on the pH value. interactions these compounds with several double‐stranded (ds) DNA dsRNA investigated means UV/Vis CD spectroscopy as well isothermal titration microcalorimetry (ITC). These studies revealed that binding to polynucleotides is driven three factors, presence aliphatic...
In order to increase the effectiveness of cancer treatment, new compounds with potential anticancer activities are synthesized and screened. Here we present screening a class compounds, 1-(2-picolyl)-, 4-(2-picolyl)-, 1-(2-pyridyl)-, 4-(2-pyridyl)-3-methyl-1,2,3-triazolium salts 'parent' 1,2,3-triazole precursors.Cytotoxic activity was determined by spectrophotometric MTT assay on several tumour one normal cell line. Effect selected compound bind double stranded DNA (ds DNA) examined testing...
The binding interactions of six ligands, neutral and monocationic asymmetric monomethine cyanine dyes comprising benzoselenazolyl moiety with duplex DNA RNA G-quadruplex structures were evaluated using fluorescence, UV/Vis (thermal melting) circular dichroism (CD) spectroscopy. main objective was to assess the impact different substituents (methyl vs. sulfopropyl thiopropyl/thioethyl) on nitrogen atom benzothiazolyl chromophore various nucleic acid structures. methyl showed a 100-fold...
At submicromolar concentrations two novel phenanthridine biguanides exhibit distinctly different spectroscopic signals for dGdC and dAdT sequences, respectively, by opposite fluorimetric changes (quenching increase dAdT) especially the bis-biguanide derivative gives an ICD response (negative dGC strong positive dAdT). This specific signalling was explained ability of compounds to switch binding mode from intercalation into minor groove sequences. Both bind rArU intercalation, yielding CD in...
The endemic Croatian species Centaurea ragusina L., like other from the genus Centaurea, has been traditionally used in Croatia as an antibacterial agent and for treatment of gastrointestinal urogenital disorders. In several chromatographic steps, three flavonoids sesquiterpene lactones were isolated identified most active fractions ethanol extract. Two lactones, one which we created trivial name ragusinin, hemistepsin A are here reported first time constituents Centaurea. All six compounds...
Among three novel DBTAA derivatives only the DBTAA–propyl–adenine conjugate 1 showed recognition of consecutive oligo dT sequence by increased affinity and specific induced chirooptical response in comparison to other single stranded RNA DNA; whereby particular importance is up until now unique efficient differentiation between rU. At variance, its close analogue DBTAA–hexyl–adenine 2 did not reveal any selectivity ss-DNA/RNA pointing out important role steric factors (linker length);...
Newly designed pentacyclic benzimidazole derivatives, featuring amino or amido side chains, were synthesized to assess their in vitro antiproliferative activity. Additionally, we conducted investigations into direct interaction with nucleic acids, aiming uncover potential mechanisms of biological action. These compounds using conventional organic synthesis methodologies, alongside photochemical and microwave-assisted reactions. Upon synthesis, the newly derived underwent testing for effects...