A5027866126- Wheat and Barley Genetics and Pathology
- Insect Resistance and Genetics
- Genetics and Plant Breeding
- Crop Yield and Soil Fertility
- Plant tissue culture and regeneration
- Genetic Mapping and Diversity in Plants and Animals
- Plant Genetic and Mutation Studies
- Transgenic Plants and Applications
- Insect symbiosis and bacterial influences
- Plant Stress Responses and Tolerance
- Food composition and properties
- CRISPR and Genetic Engineering
- Plant Micronutrient Interactions and Effects
- Seed Germination and Physiology
- Plant responses to elevated CO2
- Bioenergy crop production and management
- Lipid metabolism and biosynthesis
- Soybean genetics and cultivation
- Garlic and Onion Studies
- Heat shock proteins research
- Insect and Pesticide Research
- Plant Disease Resistance and Genetics
- Rice Cultivation and Yield Improvement
- Biofuel production and bioconversion
- Plant nutrient uptake and metabolism
China Agricultural University
2013-2024
State Key Laboratory of Plant Physiology and Biochemistry
2023
China Rural Technology Development Center
2016
Beijing University of Agriculture
2016
Beijing Academy of Agricultural and Forestry Sciences
2003-2008
Heat stress, a major abiotic stressor of wheat (Triticum aestivum L.), often results in reduced yield and decreased quality. In this study, proteomic method, Tags for Relative Absolute Quantitation Isobaric (iTRAQ), was adopted to analyze the protein expression profile changes among cultivar Jing411 under heat stress. Results indicated that there were 256 different proteins expressed According result gene annotation functional classification, 239 annotated by 856 GO function entries,...
Abstract Accurate germplasm characterization is a vital step for accelerating crop genetic improvement, which remains largely infeasible crops such as bread wheat (Triticum aestivum L.), has complex genome that undergoes frequent introgression and contains many structural variations. Here, we propose genomic strategy called ggComp, integrates resequencing data with copy number variations stratified single-nucleotide polymorphism densities to enable unsupervised identification of pairwise...
Chitin is an important component of the insect exoskeleton and peritrophic membrane. synthase 1 (CHS1) a key enzyme in chitin synthesis pathway, has role molting growth. Plant-mediated RNA interference (RNAi) been used as more target-specific environmentally safe approach to prevent control agricultural insects. The aims this study were use grain aphid (Sitobion avanae) CHS1 target gene produce transgenic wheat lines for via plant-mediated RNAi.Expression levels changed at different...
Wheat aphids are major pests during the seed filling stage of wheat. Plant lectins toxic to sap-sucking such as wheat aphids. In this study, Pinellia pedatisecta agglutinin (ppa), a gene encoding mannose binding lectin, was cloned, and it shared 92.69% nucleotide similarity 94% amino acid with ternata (pta). The ppa gene, driven by constitutive phloem-specific ribulose bisphosphate carboxylase small subunit (rbcs) promoter in pBAC-rbcs-ppa expression vector, transferred into cultivar...
Wheat (Triticum aestivum L.) is one of the three major global food crops. High-temperature stress can affect its yield and quality. Studies effect high-temperature on wheat kernel development are important because they reveal stability quality lead to genetic improvement traits. In this study, isobaric tags for relative absolute quantitation (iTRAQ) method was adopted analyze changes in protein expression profile cultivars under high temperature stress. The content grain increased heat...
Starch and prolamin composition content are important indexes for determining the processing nutritional quality of wheat (Triticum aestivum L.) grains. Several transcription factors (TFs) regulate gene expression during starch protein biosynthesis in wheat. Storage activator (TaSPA), a member basic leucine zipper (bZIP) family, has been reported to activate glutenin genes is correlated synthesis related genes. In this study, we generated TaSPA-B overexpressing (OE) transgenic lines....
Abstract Heat stress substantially reduces the yield potential of wheat (Triticum aestivum L.), one most widely cultivated staple crops, and greatly threatens global food security in context warming. However, few studies have explored heat tolerance (HST)-related genetic resources wheat. Here, we identified fine-mapped a HST locus, TaHST2, which is indispensable for both vegetative reproductive stages life cycle. The studied pair near isogenic lines (NILs) exhibited diverse morphologies...
Abstract Lipase maturation factor (LMF) family proteins are required for the and transport of active lipoprotein lipases. However, specific roles LMF2 remain unknown. In this study, a grain aphid lmf2‐like gene fragment was cloned highly similar in sequence to homologous pea aphid, Acyrthosiphon pisum . An RNAi vector constructed with used wheat transformation. The expression as well growth reproduction aphids, analyzed after feeding on transgenic wheat. There were no significant differences...
WRINKLED1 (WRI1), an APETALA2 (AP2) transcription factor (TF), critically regulates the processes related to fatty acid synthesis, storage oil accumulation, and seed development in plants. However, WRI1 genes remain unknown allohexaploid bread wheat (Triticum aestivum L.). In this study, based on sequence of Arabidopsis AtWRI1, two TaWRI1Ls wheat, TaWRI1L1 TaWRI1L2, were cloned. TaWRI1L2 was closely monocotyledons clustered one subgroup with while another AtWRI3 AtWRI4. Both expressed highly...
The gelatinization and retrogradation characteristics of wheat starch affect the eating quality Chinese-style food. Rapid Visco Analyzer (RVA) parameters have been widely used as important indicators to evaluate improve starch. However, genetic basis RVA remains be further explored. In present study, a natural population was genotyped using 90K single nucleotide polymorphism (SNP) arrays, this grown in five environments were evaluated. results showed that 22,068 high-quality SNP markers...
Abstract BACKGROUND Grain aphid ( Sitobion avenae F.) is a dominant pest that limits cereal crop production around the globe. Gq proteins have important roles in signal transduction insect olfaction. Plant‐mediated RNA interference (RNAi) has been widely studied control, but its application for control wheat field requires further study. Here, we used double‐stranded (ds)RNA feeding to verify potential of selected Gqα fragments host‐mediated RNAi, and then evaluated effect RNAi on olfaction...
Pre-harvest sprouting (PHS) occurs frequently in most of the wheat cultivation area worldwide, which severely reduces yield and end-use quality, resulting substantial economic loss. In this study, quantitative trait loci (QTL) for PHS resistance were mapped using an available high-density single nucleotide polymorphism (SNP) simple sequence repeat (SSR) genetic linkage map developed from a 269 recombinant inbred lines (RILs) population Yanda 1817×Beinong 6. Using phenotypic data on two...
Sodium dodecyl sulfate-sedimentation volume is an important index to evaluate the gluten strength of common wheat and closely related baking quality. In this study, a total 15 quantitative trait locus (QTL) for sodium sulfate (SDS)-sedimentation (SSV) were identified by using high-density genetic map including 2,474 single-nucleotide polymorphism (SNP) markers, which was constructed with doubled haploid (DH) population derived from cross between Non-gda3753 (ND3753) Liangxing99 (LX99)....
Abstract Spike compactness (SC) is strongly associated with wheat (Triticum aestivum L.) grain yield. In this study, we conducted a quantitative trait locus (QTL) analysis using doubled haploid (DH) population derived from cross between two common varieties contrasting spike morphology, revealing 16 stable QTLs SC. The effect of major QTL, QSc.cau-6B.1, was validated in 231 F7 recombinant inbred lines (RILs) the same as DH population. Using residual heterozygous (RHLs), delimited...
ABSTRACT Starch biosynthesis is a critical factor in wheat ( Triticum aestivum L.) quality and yield. However, the full scope of its regulation not fully understood. Here we report that TaDL interacts with TaB3 TaNF‐YB1 to synergistically regulate starch wheat. Genome‐edited tadl mutant lines had smaller lighter grains lower total amylose contents compared wild type (WT). Correspondingly, transcript levels biosynthesis‐related genes, including TaSUS1 , TaSUS2 TaAGPL2 TaSBEIIa TaGBSSII...
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