A5101257776- Pregnancy and preeclampsia studies
- Cancer-related molecular mechanisms research
- Pelvic floor disorders treatments
- Magnesium in Health and Disease
- Bone Metabolism and Diseases
- MicroRNA in disease regulation
- Cardiac, Anesthesia and Surgical Outcomes
- Antibiotics Pharmacokinetics and Efficacy
- Pelvic and Acetabular Injuries
- Circular RNAs in diseases
- RNA Research and Splicing
- Antimicrobial Resistance in Staphylococcus
- Urological Disorders and Treatments
- Liver Disease Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- Cancer-related Molecular Pathways
- Anorectal Disease Treatments and Outcomes
- Birth, Development, and Health
- Cell Adhesion Molecules Research
- Water Quality Monitoring Technologies
- Cancer-related gene regulation
- Chronic Kidney Disease and Diabetes
- Antibiotic Resistance in Bacteria
- Bone fractures and treatments
- Hepatitis B Virus Studies
Affiliated Hospital of Southwest Medical University
2017-2025
Guangzhou Medical University
2024
Suzhou Municipal Hospital
2020-2024
Hunan Children's Hospital
2018-2024
Nanjing Medical University
2020-2024
Ningxia University
2024
Third Affiliated Hospital of Guangzhou Medical University
2024
Ningxia Water Conservancy
2024
Nanjing Agricultural University
2023
Southern Medical University
2018-2022
Long non-coding RNA (LncRNA) contributes to the occurrence and development of osteosarcoma (OS), although underlying mechanism is not clear. In present study, we showed that lncRNA MRPL23-AS1 was remarkably increased in OS tissues cell lines. Stable knockdown evidently attenuated viability invasive ability, meanwhile inhibited vivo tumor growth dissemination. terms mechanism, luciferase reporter, pull-down fluorescence situ hybridization (FISH) assays competitively interacted with miR-30b,...
In order to investigate the effects of RhoA/ROCK signaling in macrophage differentiation, we used 100 ng/mL PMA induce differentiation from U937 cells vitro. The observation cell morphology and expression CD68 SR-A were performed confirm induced by PMA. Western blot analysis showed that ROCK1 ROCK2 phosphorylation MYPT1 significantly increased after treatment. Pulldown assay activation RhoA was obviously enhanced when treated with further demonstrate whether could mediate PMA, successfully...
Abstract Rheumatoid arthritis (RA) is a chronic autoimmune inflammatory disease, and the role of HOXA transcript at distal tip (HOTTIP) in its pathogenesis remains underexplored. This study investigates mechanism by which HOTTIP influences apoptosis response fibroblast‐like synoviocytes (FLS). An RA mouse model was established, clinical scores were analyzed. Pathological changes synovial tissues, bone mineral density (BMD) paws, serum tartrate‐resistant acid phosphatase (TRAP) activity,...
Abstract This study explored the possible effect of transcription factor GTF2I on differentiation osteoclasts and its regulation miR‐134‐5p/MAT2A axis. RANKL‐induced were measured for expressions GTF2I, miR‐134‐5p, MAT2A. The number size assessed after TRAP staining. osteoclast biomarkers, NFATC1, TRAP, CTSK, detected as well. relationships GTF2I/miR‐134‐5p/MAT2A axis verified by ChIP, dual luciferase, RNA pull‐down assay. In vivo experiments conducted ovariectomized (OVX)‐treated mice to...
With the aging population increases, senile osteoporosis has become a global public health problem. Previous evidence shown that miR-124 important effects on occurrence and development of osteoporosis. However, role in process osteoclastogenesis is still obscure.First all, we measured expression level bone marrow monocytes (BMMs) osteoporotic mice (ovariectomized mice: OVX). Next, evaluated alteration during osteoclast differentiation BMMs. Then, BMMs were transfected with mimics or...
Abstract IL-6 produced by human fibroblast-like synoviocytes (HFLS) promotes rheumatoid arthritis (RA), while lncRNA DILC regulates liver cancer stem cells inhibiting IL-6. Therefore, may participate in RA. In the present study, we found that plasma was down-regulated, up-regulated RA patients than healthy controls. Plasma levels of and were significantly inversely correlated only patients. Overexpression resulted promoted apoptosis HFLS isolated from patients, siRNA silencing played an...
Abstract Objective This study aimed to compare the diagnostic value of single or combined applications transient elastography (TE) and multivariate indicators with biopsy for detection liver fibrosis in children caused by chronic hepatitis B (CHB). Methods included 148 CHB treated at Hunan Children’s Hospital from January 1st 2015 December 31st 2018, aged 0.83 14.58 years old. All patients underwent (LB), which 43 TE. Multiple clinical data, including aspartate aminotransferase (AST),...
Osteoporosis is the imbalance in bone homeostasis between osteoblasts and osteoclasts. In this study, we investigated effects of circ_0029463/miR-134-5p/Rab27a axis on RANKL-induced osteoclast differentiation.
The expression of the metastasis suppressor gene KISS-1 in osteosarcoma cells during apoptosis and autophagy was evaluated. MG-63 were transfected with either overexpression or knockdown vector vitro, compared cell lines empty vector. After 12, 24, 48 72 h culture, proliferation examined. MTT method used to detect by flow cytometry, mRNA levels markers caspase-3, Bcl-2, Bax, LC3 Beclin1 assessed RT-PCR. Our results showed that control low group kept proliferating culture period h, while...
This study used Rho-associated protein kinase (ROCK) isoform-selective suppression or a ROCK inhibitor to analyze the roles of ROCK1 and ROCK2 in regulating endothelial dysfunction triggered by oxidized low-density lipoprotein (oxLDL).ROCK1 expression human umbilical vein cells (HUVECs) was suppressed small interfering RNA (siRNA). HUVECs were pretreated with 30 μM Y27632 (pan inhibitor) for min before exposure 200 μg/mL oxLDL an additional 24 h. Cell viability determined MTT assay, cell...
This study aimed to assess the predictive capacity of emerging serological markers, serum HBV RNA and HBcrAg, for HBeAg seroconversion in children with HBeAg-positive chronic hepatitis B (CHB). Treatment-naïve CHB who admitted Liver Disease Center Hunan Children's Hospital between April 2021 September 2022 received treatment combined entecavir interferon-alpha were recruited. Serum HBcrAg measured at baseline Weeks 12, 24, 48 treatment. Our showed that (HR = 0.71, 95% CI: 0.56-0.91, p...
Ubiquitin C-terminal hydrolase L1 (UCHL1), which is a deubiquitinating enzyme, known to play role in chemoresistance cancers. However, its potential roles and mechanisms the of breast cancer (BC) remain unclear. In this study, we examined expression patients with BC employed Kaplan–Meier analysis log-rank test for survival analyses. It was found that up-regulated UCHL1 positively associated both poor prognosis, especially HER2+ BC. Moreover, elevated cells (SK-BR-3 BT474). Similarly,...
The aim of this study was to investigate the effects Rho GDP dissociation inhibitor (RhoGDI) on TGFβ1-mediated vascular adventitia myofibroblast transdifferentiation and inhibition ROCK inhibitors. Myofibroblast remodeling model were induced by TGFβ1 in vitro balloon injury vivo. H&E (Hematoxylin & Eosin) PSR (Picrosirius Red) staining used observe morphology while immunofluorescence, immunohistochemistry, Western blotting measure protein expression. Fasudil treatment reduced expression...
Numerous studies have established that the hypoxic conditions within ovarian follicles induce apoptosis in granulosa cells (GCs), a pivotal hallmark of follicular atresia. Melatonin (N-acetyl-5-methoxytryptamine, MT), versatile antioxidant naturally present fluid, acts as safeguard for maintaining GCs’ survival during stress exposure. In this study, we unveil an innovative protective mechanism melatonin against hypoxia-triggered GC by selectively inhibiting mitochondrial ROS (mtROS)...