Abstract Celastrol, a pentacyclic tritepene extracted from Tripterygium Wilfordi plant, showing potent liver protection effects on several liver‐related diseases. However, the anti‐inflammatory potential of celastrol in fibrosis and detailed mechanisms remain uncovered. This study was to investigate effect further reveal celastrol‐induced with focus AMPK‐SIRT3 signalling. Celastrol showed ameliorative both activated hepatic stellate cells (HSCs) fibrotic liver. remarkably suppressed...

Abstract The α2,3‐sialylation of N‐glycans is considered important but complicated because the functions three β‐galactoside α2,3‐sialyltransferases, ST3GAL3, ST3GAL4, and ST3GAL6, could be compensating for one another. To distinguish their specific functions, we established each individual knockout (KO) cell line. Loss either ST3GAL3 or ST3GAL6 genes decreased proliferation colony formation, as opposed to effect in ST3GAL4 KO cells. phosphorylation levels ERK AKT were significantly...

Acute kidney injury (AKI) is a common side effect of acetaminophen (APAP) overdose. Dihydromyricetin (DHM) the most abundant flavonoid in rattan tea, which has wide range pharmacological effects. In current study, APAP-induced AKI models were established both vivo and vitro. The results showed that DHM pretreatment remarkably alleviated by promoting antioxidant capacity through nuclear factor erythroid-related 2 (Nrf2) signaling pathway vivo. addition, reduced ROS production mitochondrial...

The N-glycosylation of integrin α5β1 is thought to control many fundamental aspects cell behavior, including adhesion and migration. However, the mechanism how N-glycans function remains largely obscure. Here, we used a loss-of-function approach. Wild-type (WT) α5 mutant S3-5 (sites 3 5) α5, which contains fewer N-glycans, were stably reconstituted in knockout cancer cells. We found that migration ability cells was decreased comparison with WT. Interestingly, levels phosphorylated focal...

Hydrogen sulfide (H2S) is a gasotransmitter with variety of cardiovascular protective effects. Sirtuin 3 (SIRT3) closely related to mitochondrial function and oxidative stress. We found that NaHS increased SIRT3 expression in the preventive effect on isoproterenol- (ISO-) induced myocardial hypertrophy. further investigated whether exogenous H2S supplement improved ISO-induced hypertrophy SIRT3-dependent manner. 10-week-old male 129S1/SvImJ (WT) mice knockout (KO) were intraperitoneally...

Aberrant N-glycan sialylation of glycoproteins is closely associated with malignant phenotypes cancer cells and metastatic potential, which includes cell adhesion, migration, growth. Recently, phosphatidylinositol 4-kinase IIα (PI4KIIα), localized to the trans-Golgi network, was identified as a regulator Golgi phosphoprotein 3 (GOLPH3) vesicle transport in apparatus. GOLPH3 target PI4KIIα helps anchor sialyltransferases thereby regulates surface receptors. However, how PI4KIIα-mediated...

O-GlcNAcylation is a post-translational modification of protein serine or threonine residue catalyzed by O-GlcNAc transferase (OGT) in the nucleus and cytoplasm. plays important roles cellular signaling that affect different biological functions cells, depending upon cell type. However, whether not regulates adhesion migration remains unclear. Here, we used doxycycline-inducible short hairpin RNA (shRNA) system to establish an OGT knockdown (KD) HeLa line found key regulator for adhesion,...

Purpose: This study investigated the role of S100 calcium binding protein A4 (S100A4) in corneal wound healing and underlying mechanism S100A4-mediated PI3K/Akt/mammalian target rapamycin (mTOR) pathway. Methods: The rabbit alkali burn model was established vivo. S100A4 expression, healing, inflammation, autophagy cornea after were detected. NaOH-treated stromal cells (rCSCs) transfected with overexpressed or silencing to examine effect on vitro. cell viability, proliferation, migration,...

Myocardial ischemia or hypoxia can induce myocardial fibroblast proliferation and fibrosis. Hydrogen sulfide (H2S) is a gasotransmitter with multiple physiological functions. In our present study, primary cardiac fibroblasts were incubated H2S donor sodium hydrosulfide (NaHS, 50 μM) for 4 h followed by stimulation (containing 5% CO2 1% O2) h. Then, the preventive effects on possible mechanisms investigated. Our results showed that NaHS reduced number, decreased hydroxyproline content;...

Sirtuin 3 (SIRT3) is critical in mitochondrial function and oxidative stress. Our present study investigates whether hydrogen sulfide (H2S) attenuated myocardial fibrosis explores the possible role of SIRT3 on protective effects. Neonatal rat cardiac fibroblasts were pretreated with NaHS followed by angiotensin II (Ang II) stimulation. was knocked down siRNA technology. promoter activity expression, as well function, measured. Male wild-type (WT) knockout (KO) mice intraperitoneally injected...

Fms-like tyrosine kinase 3 (FLT3) is a glycoprotein, that member of the class III receptor family. Approximately one-third acute myeloid leukemia (AML) patients have mutations this gene, and activation FLT3 downstream pathway plays an important role in both normal malignant hematopoiesis. However, N-glycosylation for remains unclear. In study, we showed N-glycan structures on wild type (WT), internal tandem duplication (ITD), domain (TKD) mutants were different. Interestingly, expression...

We study the impact of COVID-19 pandemic shock on household consumption in China Using survey data, we find that proportion liquidity-constrained households increases quickly, but constraint levels vary across distinct groups build a heterogeneous agent life cycle incomplete market model to analyze long-run and short-run effects The quantitative results reveal slow recovery due three reasons: hiking unemployment rate, declining labor productivity, worsening income stability rate plays key...

Fucosylation is a common glycan terminal modification, which has been reported to be inhibited by 2-fluorofucose (2FF) both in vivo and vitro. The present study aimed investigate the effect of 2FF on acetaminophen (APAP)-induced acute liver injury, further clarified possible mechanisms. In study, inhibition fucosylation relieved APAP-induced injury vivo. Pretreatment with remarkably suppressed oxidative stress mitochondria damage. markedly enhanced nuclear translocation factor erythroid...

Background:There is a great need for developing novel therapies to treat liver fibrosis. Previous studies showed that both Smad7 and uPA were inhibitors of Therefore, we explored the therapeutic effects combinational gene therapy with on CCl4-induced fibrosis.Material/Methods:Smad7 genes cloned into an adenovirus vector. To observe coexpression genes, recombinant delivered CCL4-induced fibrosis models. Fibrillar collagen, hydroxyproline, α-SMA, TGF-β1, MMP-13, TIMP-1, HGF PCNA detected...

Sirtuin3 (SIRT3) plays an important role in maintaining normal mitochondrial function and alleviating oxidative stress. After carbon tetrachloride (CCl4 ) administration, the expression of SIRT3 decreased liver mice, which indicated that might play a crucial during chemical-induced acute hepatic injury. To verify hypothesis, CCl 4 was given to induce injury knockout (KO) mice wild-type (WT) mice. -induced more severe KO compared with WT In addition, stress induced by enhanced Furthermore,...

Fucosylation is one of the most important glycan terminal modifications that affects multiple biological activities proteins. 2-Fluorofucose (2FF), its specific inhibitor, has recently been reported to reveal numerous effects by blocking fucosylation both in vitro and vivo. The current study aimed evaluate effect 2FF on hydrogen peroxide (H2O2)-induced oxidative damage vitro. In our study, treatment with H2O2 increased level fucosylation, improved cell viability H2O2-treated HepG2 cells. Our...

Objective To investigate the method of essential medicines selection in China. The angiotensin II receptor antagonist (ARB) drugs were taken as templet. Methods ARB recommended byGuidelines for prevention and control hypertension China 2010including losartan, valsartan, irbesartan, telmisartan, candesartan olmesartan included intended evaluating drugs. Each drug's information (I), safety (S), administration restriction frequency drug (af), efficacy (E) scores evaluated respectively...