A5083700304- Mosquito-borne diseases and control
- HIV Research and Treatment
- Hepatitis C virus research
- Virus-based gene therapy research
- interferon and immune responses
- Herpesvirus Infections and Treatments
- SARS-CoV-2 and COVID-19 Research
- Malaria Research and Control
- Hepatitis B Virus Studies
- Animal Virus Infections Studies
- Ubiquitin and proteasome pathways
- Viral Infections and Vectors
- Toxin Mechanisms and Immunotoxins
- Plant-Microbe Interactions and Immunity
- Computational Drug Discovery Methods
- Immune Cell Function and Interaction
- Insect symbiosis and bacterial influences
- Microbial Natural Products and Biosynthesis
- HIV/AIDS drug development and treatment
- COVID-19 Clinical Research Studies
- Virology and Viral Diseases
- Viral gastroenteritis research and epidemiology
- Fungal Biology and Applications
- RNA regulation and disease
- HIV/AIDS Research and Interventions
University of Arizona
2021-2024
Wadsworth Center
2020-2021
New York State Department of Health
2020-2021
Wayne State University
2016-2020
University of Alberta
2014-2016
National Institute of Immunology
2008-2010
Pondicherry University
2006
Isolation and characterization of a bacterial isolate (strain FP10) from banana rhizosphere with innate potential as fungal antagonist microbial adjuvant in micropropagation banana.Bacterium FP10 was isolated the identified Pseudomonas aeruginosa based on phenotypic, biochemical traits sequence homology partial 622-bp fragment 16S ribosomal DNA (rDNA) amplicon, database sequences. Strain displayed antibiosis towards fungi causing wilt root necrosis diseases banana. Production plant growth...
Flaviviruses causes significant human disease. Recent outbreaks of the Zika virus highlight need to develop effective therapies for this class viruses. Previously we identified niclosamide as a broad-spectrum inhibitor flaviviruses by targeting interface between viral protease NS3 and its cofactor NS2B. Here, screened small library derivatives new analogue with improved pharmacokinetic properties. Compound JMX0207 showed efficacy in inhibition molecular interaction NS2B, better function,...
SARS-CoV-2 has caused a global pandemic with significant humanity and economic loss since 2020. Currently, only limited options are available to treat infections for vulnerable populations. In this study, we report universal fluorescence polarization (FP)-based high throughput screening (HTS) assay SAM-dependent viral methyltransferases (MTases), using fluorescent SAM-analogue, FL-NAH. We performed the against reference MTase, NSP14, an essential enzyme methylate N7 position of 5'-RNA...
Many flaviviruses including the Dengue virus (DENV), Zika (ZIKV), West Nile virus, Yellow Fever and Japanese encephalitis are significant human pathogens, unfortunately without any specific therapy. Here, we demonstrate that methylene blue, an FDA-approved drug, is a broad-spectrum potent antiviral against both in vitro vivo. We found blue can considerably inhibit interactions between viral protease NS3 its NS2B co-factor, activity, growth, protect 3D mini-brain organoids from ZIKV...
Dictyostelium discoideum exhibits the largest repository of polyketide synthase (PKS) proteins all known genomes. However, functional relevance these in biology this organism remains largely obscure. On basis computational, biochemical, and gene expression studies, we propose that multifunctional PKS (DiPKS) protein DiPKS1 could be involved biosynthesis differentiation regulating factor 4-methyl-5-pentylbenzene-1,3-diol (MPBD). Our cell-free reconstitution studies a novel acyl carrier Type...
Zika virus (ZIKV) causes significant human diseases without specific therapy. Previously we found erythrosin B, an FDA-approved food additive, inhibited viral NS2B-NS3 interactions, leading to inhibition of ZIKV infection in cell culture. In this study, performed pharmacokinetic and vivo studies demonstrate the efficacy B against 3D mini-brain organoid mouse models. Our results showed that is very effective abolishing replication model. Although pharmacokinetics indicated had a low...
Flavivirus infections, such as those caused by dengue virus (DENV), West Nile (WNV), yellow fever (YFV), and Zika (ZIKV), pose a rising threat to global health. There are no FDA-approved drugs for flaviviruses, although small number of flaviviruses have vaccines. For or unknown viruses that may appear in the future, it is particularly desirable identify broad-spectrum inhibitors. The NS5 protein regarded one most promising flavivirus drug targets because conserved across flaviviruses. In...
Adenoviruses (Ad) are commonly used as vectors for gene therapy and/or vaccine delivery. Recombinant Ad being tested vaccines many pathogens. We have made a surprising observation that peptides derived from various hepatitis C virus (HCV) antigens contain extensive regions of homology with multiple adenovirus proteins, and conclusively demonstrate vector can induce robust, heterologous cellular humoral immune responses against HCV antigens. Intriguingly, the induction this cross-reactive...
HSV-1 ICP0 is a multifunctional immediate early protein key to effective replication in the lytic cycle and reactivation latent cycle. transactivates gene expression by orchestrating an overall mitigation host intrinsic/innate restrictions. How coordinates its multiple active domains diverse protein-protein interactions question understanding life pathogenesis. The present study focuses on delineating regulatory effects of SUMO-SIM interaction E3 ubiquitin ligase activity regarding PML II...
Flaviviruses cause a significant amount of mortality and morbidity, especially in the area where they are endemic. A recent example is outbreak Zika virus though out world. Development antiviral drugs against different viral targets as important development vaccine. During replication, flavivirus genome translated single polyprotein precursor, which must be cleaved into individual proteins by complex protease, NS3, its cofactor, NS2B. Flavivirus protease most attractive target for...
Hepatitis C virus (HCV) leads to chronic infection in the majority of infected individuals due lack, failure, or inefficiency generated adaptive immune responses. In a minority patients, acute is followed by viral clearance. The correlates clearance are not clear yet but have been extensively investigated, suggesting that multispecific and multifunctional cellular immunity involved. generation highly dependent upon how antigen presenting cells (APCs) process present various antigens. Various...
Infected cell protein 0 (ICP0) of herpes simplex virus 1 (HSV-1) is an α gene product required for viral replication at low multiplicities infection. Upon entry, nuclear domain 10 (ND10) converges the incoming DNA and represses expression. ICP0 contains a RING-type E3 ubiquitin ligase that degrades ND10 organizer PML disperses to alleviate repression. In present study, we focused on understanding regulation activity in degradation different substrates. We report following. (i) A SUMO...
Emergence of newer variants SARS-CoV-2 underscores the need for effective antivirals to complement vaccination program in managing COVID-19. The multi-functional papain-like protease (PLpro) is an essential viral protein that not only regulates replication but also modulates host immune system, making it a promising therapeutic target. To this end, we developed vitro interferon stimulating gene 15 (ISG15)-based Förster resonance energy transfer (FRET) assay and screened National Cancer...
Infected cell protein 0 (ICP0) of herpes simplex virus 1 (HSV-1) is an immediate early containing a RING-type E3 ubiquitin ligase. It targets several host factors for proteasomal degradation and subsequently activates viral expression. ICP0 has nuclear localization sequence functions in the nucleus during infection. However, later infection, found solely cytoplasm. The molecular mechanism biological function nuclear-to-cytoplasmic translocation are not well understood. In this study, we...
Multispecific, broad, and potent T cell responses have been correlated with viral clearance in hepatitis C virus (HCV) infection. However, the majority of infected patients develop chronic infection, suggesting that natural infection mostly leads to development inefficient immunity. Multiple mechanisms immune modulation evasion shown HCV through various investigations. This study examined generation against core frameshift (F) proteins HCV. A single immunization mice replication incompetent...
HIV-1 Rev protein regulates the expression of transcripts by binding to a highly structured stem loop structure called Responsive Element (RRE) present in genomic and partially spliced RNAs. Genetic variation this is likely affect ultimately overall gene replication. We characterized RRE sequences from 13 infected individuals North India which also included two mother-child pairs following vertical transmission. observed high degree conservation sequences, including 9-nt (CACUAUGGG) long...
Hepatitis B and C (HBV HCV) are hepatotropic viruses in humans with approximately 350 170 million chronic carriers respectively. Since both have similar modes of transmission, many people co-infected. Co-infection is common intravenous drug users, HIV-positive individuals, transplant recipients. Compared to mono-infected patients, co-infected patients exhibit exacerbated liver cirrhosis, hepatocellular carcinoma, failure. Some the pathogenic effects may be attributed part structural core...
UBA5 is a critical E1-activating enzyme in the UFMylation pathway, post-translational modification process implicated neurodegenerative diseases and cancers. In this study, we developed high-throughput screening (HTS) assay to identify inhibitors of from library blood-brain barrier (BBB)-permeable compounds. The assay, based on AMP-Glo kit, enabled identification five novel belonging three distinct chemical scaffolds with low micromolar IC50 values. These demonstrated selectivity for over...