A5032225816- Polysaccharides and Plant Cell Walls
- Advanced Cellulose Research Studies
- Enzyme Production and Characterization
- Glycosylation and Glycoproteins Research
- Plant nutrient uptake and metabolism
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Adsorption and biosorption for pollutant removal
- Electrochemical sensors and biosensors
- Polysaccharides Composition and Applications
- Algal biology and biofuel production
- Protein purification and stability
- Water Quality Monitoring and Analysis
- Viral Infectious Diseases and Gene Expression in Insects
- Plant Molecular Biology Research
- Biocrusts and Microbial Ecology
- Microbial bioremediation and biosurfactants
- Plant Pathogens and Fungal Diseases
- Monoclonal and Polyclonal Antibodies Research
- Microbial Community Ecology and Physiology
- Force Microscopy Techniques and Applications
- Microbial Metabolic Engineering and Bioproduction
- Nanoparticle-Based Drug Delivery
Rutgers, The State University of New Jersey
2023-2025
Indian Institute of Technology Guwahati
2017-2020
Cellulose, the most abundant polysaccharide on earth composing plant cell walls, is synthesized by coordinated action of multiple enzymes in cellulose synthase complexes embedded within plasma membrane. Multiple chains fibrils form intertwined extracellular matrix networks. It remains largely unknown how newly assembled into an intricate fibril network surfaces. Here, we have established vivo time-resolved imaging platform to continuously visualize biosynthesis and assembly Arabidopsis...
Abstract Carbohydrate binding modules (CBMs) are noncatalytic domains that assist tethered catalytic in substrate targeting. CBMs have therefore been used to visualize distinct polysaccharides present the cell wall of plant cells and tissues. However, most previous studies provide a qualitative analysis CBM‐polysaccharide interactions, with limited characterization engineered tandem CBM designs for recognizing like cellulose application CBM‐based probes fibrils synthesis model protoplasts...
In the present study, we have demonstrated application of two-step optimization strategy for hexavalent chromium (Cr (VI)) adsorption using Sargassum sp in a packed bed column. ANOVA showed that initial metal concentration has larger impact on sorption efficiency. Application ANN-GA promising results among models tested. The biosorption efficiency accelerated to 90.79% with Cr (VI) conc = 25 mg L−1, Bed height 11.97 cm and Flow rate 5.29 mL min−1. mechanism was described kinetic where depth...
Abstract Polysaccharides are a major class of natural polymers found abundantly across all life forms and play critical role as structural, metabolic, or functional components in biomolecular processes. Some polysaccharides like cellulose hyaluronan synthesized by membrane-bound family-2 glycosyltransferases (GTs). Despite the fact that GT-2 family has maximum number deposited sequences, biochemistry enzymes is still poorly understood due to difficulties associated with GT membrane protein...
Summary Carbohydrate binding modules (CBMs) are non-catalytic domains associated with cell wall degrading carbohydrate-active enzymes (CAZymes) that often present in nature tethered to distinct catalytic (CD). Fluorescently labeled CBMs have been also used visualize the presence of specific polysaccharides plant cells and tissues. Previous studies provided a qualitative analysis CBM-polysaccharide interactions, limited characterization optimal CBM designs for recognizing glycans....
Plant cell walls are composed of polysaccharides among which cellulose is the most abundant component. Cellulose processively synthesized as bundles linear β-1,4-glucan homopolymer chains via coordinated action multiple enzymes in synthase complexes (CSCs) embedded within plasma membrane. layers fibrils that form highly intertwined extracellular matrix networks. However, it not yet clear to how by CSCs assembled into intricate network deposited on plant surfaces. Herein, we have established...
Abstract The cooperative effect of multiple affinity binding interactions creating a stable bond, known as avidity, is particularly important in assessing the potency potential drugs such monoclonal antibodies, CAR T, or NK cells to treat cancer. However, predicting avidity based on vitro single has limitations and often fails describe effects observed vivo . Acoustic force-based assays have recently emerged reliable method for direct measurements, expressed adhesion forces, which positively...
Abstract The most abundant renewable biopolymer on earth, viz., cellulose, acts as carbon storage reserve in plant and microbial cell walls that could potentially be converted into biofuels or other valuable bioproducts. Cellulose is synthesized by a membrane-integrated processive glycosyltransferase (GT) called cellulose synthase (CesA). Since only few of these CesAs have been purified characterized to date, there are huge gaps our mechanistic understanding enzymes. Furthermore, the...