A5102781868- Viral Infections and Outbreaks Research
- 3D Printing in Biomedical Research
- Chromatography in Natural Products
- Immune cells in cancer
- Olfactory and Sensory Function Studies
- Circadian rhythm and melatonin
- Wireless Body Area Networks
- Allergic Rhinitis and Sensitization
- Molecular Communication and Nanonetworks
- Epigenetics and DNA Methylation
- Phagocytosis and Immune Regulation
- Neurobiology and Insect Physiology Research
- Inflammatory mediators and NSAID effects
- Food Allergy and Anaphylaxis Research
- Natural product bioactivities and synthesis
- Traditional Chinese Medicine Analysis
- Tryptophan and brain disorders
- Liver Disease Diagnosis and Treatment
- Computational Drug Discovery Methods
- Biomedical and Engineering Education
- Influenza Virus Research Studies
- Osteoarthritis Treatment and Mechanisms
- Asthma and respiratory diseases
- Metabolomics and Mass Spectrometry Studies
- Cellular Mechanics and Interactions
Leiden University
2019-2025
Centre for Human Drug Research
2019-2023
Beijing University of Chinese Medicine
2016-2017
Ebola virus, for which we lack effective countermeasures, causes hemorrhagic fever in humans, with significant case fatality rates. Lack of experimental human models is a major obstacle that hinders the development treatment strategies. Here, model syndrome microvessel-on-a-chip system and demonstrate its applicability to drug studies. Luminal infusion virus-like particles leads albumin leakage from engineered vessels. The process mediated by Rho/ROCK pathway associated cytoskeleton...
As the most familiar type of arthritis and a chronic illness joints, Osteoarthritis (OA) affects great number people on global scale. XuanHuSuo powder (XHSP), conventional herbal formula from China, has been extensively applied in OA treatment. Nonetheless, its pharmacological mechanism not completely expounded. In this research, network pharmacology approach chosen to study XHSP OA, networks were established based relationship between four herbs found XHSP, compound targets, targets. The...
Capillary leakage is a hallmark of severe dengue, yet its precise mechanisms remain elusive. Emerging evidence highlights the dengue virus's targeting mechanically active endothelial cells as key contributor to shock syndrome. The viral nonstructural protein 1 (NS1) has been identified central player, disrupting integrity and inducing vascular hyperpermeability independently pro-inflammatory cytokines. This study provides direct assessment NS1-induced pathology by combining single-cell force...
Macrophages remodel their mechanics during differentiation toward different subtypes and drastically adapt shapes phagocytosis or entry to inflamed tissues. Although these functions depend on cell mechanical properties, the behavior of macrophages is still poorly understood accurate physiologically relevant data basic properties macrophage are lacking almost entirely. By combining several complementary single‐cell force spectroscopy techniques, whole M1 (differentiated by granulocyte...
Abstract Human macrophages are innate immune cells with diverse, functionally distinct phenotypes, namely, pro‐inflammatory M1 and anti‐inflammatory M2 macrophages. Both involved in multiple physiological pathological processes, including would healing, infection, cancer. However, the metabolic differences between these phenotypes largely unexplored at single‐cell resolution. To address this knowledge gap, an untargeted live mass spectrometry‐based metabolomic profiling coupled a...
Abstract Lack of experimental human models hinders research on Lassa hemorrhagic fever and the development treatment strategies. Here, we report first chip‐based model for syndrome. The chip features a microvessel interfacing collagen network as simple mimic extracellular matrix, allowing quantitative real‐time vascular integrity assessment. Luminal infusion virus‐like particles led to dramatic increase in permeability viral load‐dependent manner. Using this platform, showed that...
Fungi of the Alternaria genus are associated with allergic diseases, alternata being one most prevalent species. A. has been frequently reported as etiologic agent hypersensitivity pneumonitis, rhinosinusitis, bronchial asthma, and other diseases. In this study, we developed a loop-mediated isothermal amplification (LAMP) assay real-time PCR to detect low levels in herbal tea samples. The LAMP can little 3 pg/μL genomic DNA high specificity. addition, both be used for quantification...
Abstract Ebola virus can trigger a release of pro-inflammatory cytokines with subsequent vascular leakage and impairment clotting finally leading to multiorgan failure shock after entering infecting patients. is known directly target endothelial cells macrophages, even without them, through direct interactions viral proteins. These affect cellular mechanics immune processes, which are tightly linked other key functions such as metabolism. However, research regarding metabolic activity these...
Xuan Hu Suo San (XHSS) is a traditional Chinese medicine that has been extensively applied in the treatment of osteoarthritis for many years, however, its chemical composition not yet elucidated. Thus, rapid, efficient, and precise method based on ultra high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was both positive- negative-ion modes to rapidly separate identify main ingredients XHSS first time. Finally, optimised separation detection...