Maternally expressed gene 3 (Meg3) has been shown to promote tumor progression. However, the role of Meg3 in development a chemoresistant phenotype human lung cancer remains. Reverse transcription‑quantitative polymerase chain reaction analysis was used determine expression Meg3. Flow cytometric and MTT assay were also investigate cell cycle apoptosis. The present study detected that levels significantly lower cisplatin‑resistant A549/DDP cells, compared with those parental A549 cells....

Metastasis is the main cause of death in patients with advanced lung cancer. The exosomes released by cancer cells create tumor microenvironment, and then accelerate metastasis. Cancer-derived are considered to be driving force for metastasis niche formation at foreign sites, but mechanism Non-small cell carcinoma (NSCLC) unclear. In metastatic NSCLC patients, expression level miR-3157-3p circulating was significantly higher than that non-metastatic patients. Here, we found can transferred...

Aberrant expression of microRNAs has been shown to regulate the biological processes lung cancer cells. However, role miR‐638 in development NSCLC is still unclear. In this study, low and high SOX2 were be associated with tumor size metastasis patients. Downregulated could promote cell invasion proliferation, while reversed effect. Furthermore, by directly binding its 3′‐UTR. Silencing siRNA partially abolished enhancement proliferation induced downregulated miR‐638. modulate levels markers...

The involvement of miR‐204 in lung cancer development is unclear. In our study, we analyzed the expression tumor‐ and adjacent‐tissue samples from 141 patients with non‐small cell (NSCLC). MiR‐204 was decreased tumor compared non‐cancerous tissue‐derived controls. Moreover, negatively correlated homeobox protein SIX1 expression, size metastasis. silencing miR‐204‐positive NSCLC lines promoted invasion proliferation. Concomitantly, overexpression resulted reduced proliferation invasion,...

Several microRNAs (miRNA) have been implicated in H. pylori related gastric cancer (GC). However, the molecular mechanism of miRNAs has not fully understood. In this study, we reported that miR-101 is significantly down-regulated positive tissues and cells tumor with important functional consequences. Ectopic expression dramatically suppressed cell proliferation colony formation by inducing G1-phase cell-cycle arrest. We found strongly reduced SOCS2 oncogene GC cells. Similar to restoring...

Abstract Objectives Lung cancer is still a disease with high morbidity and mortality in the world. Micro RNA s have been proven to act as an indispensable role reuse of multiple solid tumours. Although miR‐1258 plays vital suppressing metastasis breast gastric cancer, specific biological function non‐small‐cell lung remains unclear. Methods The differential expression NSCLC tissues corresponding paracancerous was detected by qRT ‐ PCR ISH . Flow cytometry CCK ‐8, EdU, tubule formation,...

Localized plasmonic structured illumination microscopy (LPSIM) provides multicolor wide-field super-resolution imaging with low phototoxicity and high-speed capability. LPSIM utilizes a nanoscale antenna array to provide series of tunable patterns beyond the traditional diffraction limit, allowing for enhanced resolving powers down few tens nanometers. Here, we demonstrate 50 nm spatial resolution at video rate speed by microtubule dynamics power intensity. The design system makes it...

Abstract Increasing evidence confirms that exosome‐mediated transfer of microRNAs can influence cancer progression including tumor cell invasion, proliferation, and drug resistance via cell–cell communication. However, the potential role exosomal‐miR‐1260b in lung adenocarcinoma (LAC) remains poorly understood. Thus, this study focused on investigating function invasion. Exosomal‐miR‐1260b was found to be higher plasma patients with LAC than healthy persons quantitative real‐time polymerase...

MicroRNAs (miRNAs) have been regarded as a new class of regulators in cellular processes non-small cell lung cancer (NSCLC). However, the relationship between miR-452 and development NSCLC remains unclear.qRT-PCR was used to detect expression its target gene samples (n=60). The transwell assay test invasion capability. regulation mechanism confirmed by luciferase reporter western blot assay.In current study, relatively lower higher BMI1 levels were be associated with advanced tumor stage...

MicroRNA (miR)‑30c has been identified as a tumor suppressor gene in numerous diseases. Aberrant miR‑30c expression associated with the invasion of different types cancer. However, potential mechanisms underlying association between and poorly elucidated non‑small‑cell lung cancer (NSCLC). In present study, quantitative polymerase chain reaction demonstrated that was reduced specimens (n=85). Suppressing promoted A549 cells, while overexpressed inhibited cells. Furthermore, aberrant able to...

Recently, long non-coding RNAs (lncRNAs) have been found to many biological effects in different cancer stages. Several studies revealed that focally amplified lncRNA on chromosome 1 (FAL1) regulates progression via p21. However, the expression and mechanism of FAL1 non-small cell lung (NSCLC) still remain unclear.We detected level NSCLC tissues established lines using quantitative real-time PCR evaluated clinical significance. was silenced or overexpressed siRNA lentivirus study whether...

Background/Aims: MicroRNAs have been validated to play a crucial role in tumorigenesis of non-small cell lung cancer (NSCLC). Although miR-106b-5p has reported vital various malignancies the physiological function NSCLC still remain unknown. In this study, we investigated NSCLC. Methods: Quantitative real-time polymerase chain reaction was conducted estimate expression and BTG3 both tissues lines. The effects on proliferation were determined vitro using CCK-8 assays,...

Abstract Non-small cell lung cancer (NSCLC) is one of the most common aggressive malignancies. miRNAs have been identified as important biomarkers and regulators NSCLC. However, functional contributions miR-1260b to NSCLC proliferation apoptosis not studied. In this study, was upregulated in plasma, tissues, lines, its high expression correlated with tumor size progression. Functionally, overexpression promoted cycle, conversely inhibited senescence. Mechanically, negatively regulated SOCS6...

Purpose: The study was aimed at identifying the pathogenic gene responsible for X‐linked nonsyndromic hearing loss (NSHL) in a five‐generation Chinese family and elucidating gene’s function both vivo using zebrafish model vitro PRPS1 knockdown HEI‐OC1 cells. Methods: Exome sequencing (ES) Sanger were used to identify variants. A transgenic overexpressing novel variant (c.494G>A: p.Cys165Tyr) constructed, knocked down cells siRNA explore underlying mechanisms. Hair cell development...

Background: The connection between microRNA expression and lung cancer development has been identified in recent literature. However, the mechanism of poorly elucidated non-small-cell (NSCLC). Methods Results: Comparing with adjacent tissues (n=75), miR-30c a lower specimens (n=75). knockdown enhanced invasion A549 cells; meanwhile, overexpression could reverse effect vitro. A luciferase assay revealed that was directly bound to 3‘-untranslated regions (3‘-UTR) MTA1. QRT-PCR western blot...

MicroRNAs (miRNAs) are non-coding small RNAs that regulate cell proliferation and functions by interfering with the translation of target mRNAs. Altered expression miRNA is known to induce various human malignancies, but little about role miRNAs in esophageal squamous carcinoma (ESCC).RT-PCR Western blot were used examine candidate genes 40 pairs human. MiR-16 mimics inhibitor transfected TE-1 Eca-109 cells before detecting migration, proliferation, apoptosis cycle. The regulation mechanism...

The Wilms’ tumor suppressor gene (WT1) has been identified as an oncogene in many malignant diseases such leukaemia, breast cancer, mesothelioma and lung cancer. However, the role of WT1 non-small-cell cancer (NSCLC) carcinogenesis remains unclear. In this study, we compared mRNA levels NSCLC tissues with paired corresponding adjacent significantly higher expression specimens. Cell proliferation three cell lines positively correlated expression; moreover, these associations were both a...

Abnormal expression of microRNA-124 (miR-124) was found in non-small cell lung cancer (NSCLC). However, the association between miR-124 and CDH2 has not been reported yet. This study aims to reveal inhibiting effects on NSCLC.Quantitative real-time polymerase chain reaction used evaluate NSCLC tissues. Cell viability, apoptosis invasion assays were carried out lines after transfection. The regulation mechanism confirmed by luciferase report assay western blot (WB).Significantly decreased...

Recently, many studies showed that long noncoding RNAs (lncRNAs) are involved in tumor progression. It is reported lncRNA-LET downregulated and has antitumor effect on several types of cancer. This study focuses the role lung adenocarcinoma (LAC) RT-PCR results indicated frequent downregulation LAC tissues was related to clinicopathologic factors. knockdown significantly promoted cell proliferation, invasion, migration while overexpression obviously inhibited migration, indicating a...

Liver cancer primarily accounts for the majority of malignancies liver. MicroRNAs (miRNAs) are endogenous non-coding RNAs, which important in tumorigenesis. Abnormal expression microRNA-346 (miR-346) has been demonstrated various types human cancer, however, its and potential molecular mechanism liver remains to be elucidated. Expression levels miR-346 cell lines were determined by quantitative polymerase chain reaction. The effect on proliferation was evaluated an MTT assay; migration...

Esophageal squamous cell carcinoma (ESCC) is one of the prevalent and deadly cancers worldwide, especially in China. Considering poor prognosis ESCC, aim this study to dissect effects long non-coding RNA (lncRNA) AK001796 on proliferation cycle vitro tumorigenicity vivo, providing therapeutic targets for ESCC.We conducted quantitative real time PCR detect expression level lncRNA human ESCC tumor adjacent non-tumor tissues, analyzed correlation between clinicopathologic feature patients. Then...