A5103130456- Neuroscience and Neural Engineering
- Microfluidic and Bio-sensing Technologies
- 3D Printing in Biomedical Research
- Microbial Inactivation Methods
- Advanced Memory and Neural Computing
- Plant responses to elevated CO2
- Photoreceptor and optogenetics research
- Photosynthetic Processes and Mechanisms
- Electrochemical Analysis and Applications
- Atmospheric chemistry and aerosols
- Analytical Chemistry and Sensors
- Plant Water Relations and Carbon Dynamics
- Plant Stress Responses and Tolerance
- Electrochemical sensors and biosensors
- Microfluidic and Capillary Electrophoresis Applications
- Cardiac electrophysiology and arrhythmias
- Phonetics and Phonology Research
- Plant Surface Properties and Treatments
- Environmental and Agricultural Sciences
- Ion channel regulation and function
- Cardiovascular Effects of Exercise
- Soybean genetics and cultivation
- Receptor Mechanisms and Signaling
- Neonatal Respiratory Health Research
- Tissue Engineering and Regenerative Medicine
Children's Hospital of Zhejiang University
2024-2025
Children's Hospital
2025
Zhejiang University
2024-2025
National Clinical Research
2025
Center for Children
2025
Sun Yat-sen University
2019-2024
State Key Laboratory of Optoelectronic Materials and Technology
2022
The First Affiliated Hospital, Sun Yat-sen University
2021
Chinese Academy of Sciences
1999-2021
State Key Laboratory of Transducer Technology
2021
Nonnodulated pea (Pisum sativum L. cv Frosty) and soybean (Glycine max [L.] Merr. Wye) plants were grown under artificial lights from germination with ample nutrients, 600 [mu]mol photons m-2 s-1, either 34 to 36 (control) or 64 68 Pa (enriched) CO2. For soybean, pod removal whole-plant shading treatments used alter the source-sink balance carbohydrate status of plants. Growth both species was substantially increased by CO2 enrichment despite some down-regulation photosynthesis rate per unit...
Electrophysiology is a unique biomarker of the electrogenic cells that can perform disease investigation or drug assessment. In recent decade, vertical nanoelectrode arrays successfully achieve high-quality intracellular electrophysiological study in and their networks. However, high success rate long-term recording using low-cost nanostructures still considerable challenge. Herein, we develop scalable robust hollow nanopillar electrode to enhanced cardiomyocytes. The template-based...
Electroporation is a proven technique that can record action potential of cardiomyocytes and serve for biomolecular delivery. To ensure high cell viability, micro-nanodevices cooperating with low-voltage electroporation are frequently utilized in research, the effectiveness delivery intracellular access typically assessed using an optical imaging approach like flow cytometry. However, efficiency situ biomedical studies hampered by intricacy these analytical approaches. Here, we develop...
New strategies for intracellular electrophysiology break the spatiotemporal limitation of action potential and lead a notable advance in investigation electrically excitable cells their network. Although successful applications recording have been achieved by 3D micro/nanodevices, complex micro/nanofabrication processes preclude progress extensive applications. We address this challenge introducing porous polyethylene terephthalate (PET) membrane to develop new type nanotemplate electrode....
Bradyarrhythmia is a major cause of cardiovascular disease morbidity and mortality. Currently, medication and/or surgery are the conventional clinical therapeutic strategies for bradyarrhythmia, whereas drug side effects, invasive surgery, or potential complications limit their extensive application. Therefore, development alternative therapies bradyarrhythmia urgently needed. Herein, we propose universal efficient drug-mimicking strategy to treat which relies on photothermal properties...
Fishscale bamboo rhombic-spot, caused by Neostagonosporella sichuanensis, poses a significant threat to Phyllostachys heteroclada in Sichuan province. Based on genomic analysis, four cutinase genes, NsCut1-NsCut4, were identified, cloned, and functionally validated. Bioinformatics analyses revealed that the proteins encoded these genes possess secretory functions, lack transmembrane domains, contain conserved domains highly homologous those other fungi. Recombinant expressed via prokaryotic...
Bradyarrhythmia is a kind of cardiovascular disease caused by dysregulation cardiomyocytes, which seriously threatens human life. Currently, treatment strategies bradyarrhythmia mainly include drug therapy, surgery, or implantable cardioverter defibrillators, but these are limited side effect, surgical trauma, and instability implanted devices. Here, we developed an integrated Au-nanoroded biosensing regulating platform to investigate the photothermal therapy cardiac in vitro. electrode...
Cardiac oxidative stress is a significant phenotype of myocardial infarction disease, leading cause global health threat. There an urgent need to develop innovative therapies. Nanosized extracellular vesicle (nEV)-based therapy shows promise, yet real-time monitoring cardiomyocyte responses nEVs remains challenge. In this study, dynamic and label-free biosensing system using microelectrode arrays (MEAs) was constructed. Cardiomyocytes were cultured on MEA devices for electrophysiological...
Electrophysiological recording, as a long-sought objective, plays crucial role in fundamental biomedical research and practical clinical applications. The challenge developing electrophysiological detection platforms is to combine simplicity, stability, sensitivity the same device. In this study, we develop nanotrapped microelectrode based on porous PET membrane, which compatible with large-scale microtechnologies. nanotraps can promote protrusion of local cell membrane hollow center offer...
Background.Breast cancer has the highest incidence and mortality among all cancers in women.Paclitaxel (PTX) a notable therapeutic effect on clinical practice. Objectives.To explore mechanism of PTX proliferation, apoptosis invasiveness breast cells.Material methods.MCF-7 cells were treated with (0 μM, 0.01 0. 1 μM) for 48 h.Cell viability was detected using MTT assay lactate dehydrogenase (LDH) assay; cell proliferation rate 5-ethynyl-2'-deoxyuridine (EdU) to screen most effective...
Abstract With the advancement of micro/nanotechnologies, multielectrodes arrays (MEAs) with different three‐dimensional (3D) micro/nanostructures have been developed to achieve intracellular action potential recording cardiomyocytes. However, effect 3D density on has not fully investigated. In this work, tunable nanodensity electrode (TNDEA) are fabricated by hydrothermal synthesis and standard microfabrication explore regulation biosensing. By low‐voltage electroporation, signal quality...
Cardiovascular diseases (CVDs) are the leading cause of mortality and therefore pose a significant threat to human health. Cardiac electrophysiology plays crucial role in investigation treatment CVDs, including arrhythmia. The long-term accurate detection electrophysiological activity cardiomyocytes is essential for advancing cardiology pharmacology. Regarding study cardiac cells, many micronano bioelectric devices systems have been developed. Such bioelectronic possess unique geometric...
Bradyarrhythmia poses a serious threat to human health, with chronic progression causing heart failure and acute onset leading sudden death. In this study, we develop scalable drug-mimicking nanoplasmonic therapeutic strategy by introducing gold nanorod (Au NR) mediated near-infrared (NIR) photothermal effects. An integrated sensing regulation platform is established for in situ synchronized NIR laser electrophysiological property recording. The Au NR plasmonic enables the restoration of...
At the grain-filling stage, net photosynthetic rate (PN), stomatal conductance (gs), and ribulose-1,5-bisphosphate carboxylation efficiency (CE) were correlated in order to find determinant of capacity rice leaves. For a flag leaf, PN leaf middle region was higher than its upper region, basal had lowest value. The differences gs CE similar. PN, gs, gradually declined from leaves, showing position gradient. correlation coefficient between much that both cases, negatively with intercellular...
Net photosynthetic rate (P N) measured at the same CO2 concentration, maximum in vivo carboxylation rate, and contents of ribulose-1,5-bisphosphate (RuBP) carboxylase/oxygenase (RuBPCO) RuBPCO activase were significantly decreased, but electron transport RuBP content had no significant change CO2-enriched [EC, about 200 µmol mol-1 above ambient concentration (AC)] wheat leaves compared with those AC grown leaves. Hence acclimation to EC is largely due limitation.
Abnormal cardiac electrophysiological activities significantly contribute to the incidence of cardiovascular diseases. Therefore, it is crucial recognize effective drugs, which require an accurate, stable, and sensitive platform. Although conventional extracellular recordings offer a non-invasive label-free manner monitor state cardiomyocytes, misrepresented low-quality action potentials are difficult provide accurate high-content information for drug screening. This study presents...
Abstract Developing accurate electrophysiological recording platforms is crucial for investigating cellular electrical activities, understanding the mechanisms underlying cardiovascular disease, and advancing therapeutic strategies. While successful of intracellular status has been achieved by 3D micro/nano devices, their preparation through demanding micro/nanofabrication poorly controllable solvothermal synthesis an impediment to widespread utilization. Easier‐to‐access electrodes are...